Microbial interactions in building of communities

Wright, Christopher J.; Burns, Logan H.; Jack, Alison A.; Back, Catherine R.; Dutton, Lindsay C.; Nobbs, Angela H.; Lamont, Richard J.; Jenkinson, Howard F.

doi:10.1111/omi.12012

Cited by 162 publications

(170 citation statements)

References 156 publications

(185 reference statements)

Supporting

Mentioning

163

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have shown that P. gingivalis interacts with primary colonizing organisms such as S. gordonii 22,23 and that this interaction may be an initial event that facilitates P. gingivalis colonization of the oral cavity. As such, it represents an ideal target for therapeutic intervention to limit P. gingivalis colonization.…”

Section: Discussionmentioning

confidence: 99%

Peptide-modified nanoparticles inhibit formation of Porphyromonas gingivalis biofilms with Streptococcus gordonii

Kalia

Jain

Krishnan

et al. 2017

IJN

View full text Add to dashboard Cite

Purpose The interaction of Porphyromonas g ingivalis with commensal streptococci promotes P. gingivalis colonization of the oral cavity. We previously showed that a synthetic peptide (BAR) derived from Streptococcus gordonii potently inhibited the formation of P. gingivalis/S. gordonii biofilms (IC 50 =1.3 µM) and reduced P. gingivalis virulence in a mouse model of periodontitis. Thus, BAR represents a novel therapeutic to control periodontitis by limiting P. gingivalis colonization of the oral cavity. Here, we sought to develop drug-delivery vehicles for potential use in the oral cavity that comprise BAR-modified poly(lactic-co-glycolic)acid (PLGA) nanoparticles (NPs). Methods PLGA-NPs were initially modified with palmitylated avidin and subsequently conjugated with biotinylated BAR. The extent of BAR modification was quantified using a fluorescent-labeled peptide. Inhibition of P. gingivalis adherence to S. gordonii by BAR-modified NPs was compared with free peptide using a two-species biofilm model. Results BAR-modified NPs exhibited an average size of 99±29 nm and a more positive surface charge than unmodified NPs (zeta potentials of −7 mV and −25 mV, respectively). Binding saturation occurred when 37 nmol BAR/mg of avidin-NPs was used, which resulted in a payload of 7.42 nmol BAR/mg NPs. BAR-modified NPs bound to P. gingivalis in a dose-dependent manner and more potently inhibited P. gingivalis/S. gordonii adherence and biofilm formation relative to an equimolar amount of free peptide (IC 50 of 0.2 µM versus 1.3 µM). BAR-modified NPs also disrupted the preformed P. gingivalis/S. gordonii biofilms more effectively than free peptide. Finally, we demonstrate that BAR-modified NPs promoted multivalent association with P. gingivalis , providing an explanation for the increased effectiveness of NPs. Conclusion These results indicate that BAR-modified NPs deliver a higher local dose of peptide and may represent a more effective therapeutic approach to limit P. gingivalis colonization of the oral cavity compared to treatment with formulations of free peptide.

show abstract

Section: Discussionmentioning

confidence: 99%

Peptide-modified nanoparticles inhibit formation of Porphyromonas gingivalis biofilms with Streptococcus gordonii

Kalia

Jain

Krishnan

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…Close proximity or physical contact between two microbes can substantially alter their physiology, for example conferring on an anaerobe the ability to survive in an aerobic environment (1) or dramatically altering the range of metabolites produced compared with those produced by the same organism in isolation (2)(3)(4)(5). Thus, a mechanistic understanding of the physiology of key players depends on knowing the identity of the neighbors with which they commonly interact.…”

mentioning

confidence: 99%

Biogeography of a human oral microbiome at the micron scale

Welch

Rossetti

Rieken

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

702

557

View full text Add to dashboard Cite

The spatial organization of complex natural microbiomes is critical to understanding the interactions of the individual taxa that comprise a community. Although the revolution in DNA sequencing has provided an abundance of genomic-level information, the biogeography of microbiomes is almost entirely uncharted at the micron scale. Using spectral imaging fluorescence in situ hybridization as guided by metagenomic sequence analysis, we have discovered a distinctive, multigenus consortium in the microbiome of supragingival dental plaque. The consortium consists of a radially arranged, nine-taxon structure organized around cells of filamentous corynebacteria. The consortium ranges in size from a few tens to a few hundreds of microns in radius and is spatially differentiated. Within the structure, individual taxa are localized at the micron scale in ways suggestive of their functional niche in the consortium. For example, anaerobic taxa tend to be in the interior, whereas facultative or obligate aerobes tend to be at the periphery of the consortium. Consumers and producers of certain metabolites, such as lactate, tend to be near each other. Based on our observations and the literature, we propose a model for plaque microbiome development and maintenance consistent with known metabolic, adherence, and environmental considerations. The consortium illustrates how complex structural organization can emerge from the micron-scale interactions of its constituent organisms. The understanding that plaque community organization is an emergent phenomenon offers a perspective that is general in nature and applicable to other microbiomes.biofilm | imaging | microscopy | microbial ecology B iogeography-the study of the distribution of organisms across the globe-seeks to recognize patterns in the spatial distribution of organisms and discover the forces that underlie those patterns. Bacteria are micron-sized, and many of the forces and factors that underlie their distributional patterns operate at micron scales and are qualitatively different from the large-scale factors, such as climate, that drive traditional biogeography. To frame the analysis of microbial distribution patterns at the scale that microbes themselves experience, we introduce the concept of micron-scale biogeography: the study of the distribution of microbes relative to micron-scale features of their environment. These features include the host or inanimate surfaces on which the microbes reside as well as local gradients of nutrients and oxygen. Key components of the micron-scale environment, particularly in biofilms and other densely populated habitats, are the microbes themselves, serving as substrates for attachment of other microbes, creating spatial structure, and acting as point sources for diffusible metabolites.Micron-scale biogeography is critical to understanding the physiology and ecology of the community as well as its systems biology and its effects on human health and disease. Close proximity or physical contact between two microbes can substantially a...

show abstract

“…These species of streptococci interact with a range of other oral microorganisms, thus establishing a foundation for the building of biofilm communities (Wright et al, 2013). S. gordonii forms biofilms with Porphyromonas gingivalis (Lamont et al, 2002), Actinomyces oris (Palmer et al, 2003), Fusobacterium nucleatum (Foster & Kolenbrander, 2004) and Aggregatibacter actinomycetemcomitans in dual or mixed species systems, and also produces dual species biofilms with the fungus Candida albicans (Bamford et al, 2009;Dutton et al, 2014).…”

Section: Introductionmentioning

confidence: 99%