Microbial hosts for production of D-arabitol: Current state-of-art and future prospects

Ravikumar, Yuvaraj; Razack, Sirajunnisa Abdul; Ponpandian, Lakshmi Narayanan; Zhang, Guoyan; Yun, Jimmy; Huang, Jia‐Qi; Lee, Donghun; Li, Xiaolan; Dou, Yuan; Qi, Xianghui

doi:10.1016/j.tifs.2021.12.029

Cited by 23 publications

(14 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only the terms with significant effect (P < 0.05) and those that have significant interactions were retained in the model and reported herein of glycerol concentration and the glycerol concentration × temperature interaction were very influential on arabitol titer. Such indication on a positive major effect of glycerol is in accord with previous studies consistently indicating that high concentrations of glycerol (or other carbon source) were positively associated with arabitol production [14][15][16]18], even though a search of the optimum concentration has not been carried out so far with CCD approach. The not significant effect of DOT is also coherent with literature describing low oxygen demands during arabitol production with other yeasts, except for very low dissolved oxygen concentrations or anaerobic conditions inhibiting the polyol yield [15,19].…”

Section: Table 3 Anova Results For the Quadratic Models Describing Ar...supporting

confidence: 88%

“…These results indicated that maintaining high glycerol concentration was pivotal for triggering arabitol production by W. anomalus WC 1501, confirming the evidence of previous studies [16,17,19,20]. Another feature that this study shares with all the previous ones reporting high arabitol production is the fact that the high Representative time-courses are reported herein concentration of the carbon source was accompanied by limiting concentrations of the nitrogen source, in conditions of high C/N ratio [14][15][16][17][18][19][20][21]. Similar conclusions were obtained recent studies with the Debaryomyces prosopidis and Yarrowia lipolytica, reporting very performing fermentative processes [19,20].…”

Section: Further Improvements Of the Fed Batch Processessupporting

confidence: 91%

“…The ability of some fungi to produce arabitol from glucose is well established and efficient processes have been proposed [12][13][14]. Much less information is available on microbial processes yielding arabitol from glycerol [15][16][17][18], even though highly performing processes have been recently proposed with Debaryomyces prosopidis and Yarrowia lipolytica [19,20]. W. anomalus WC 1501 emerged as an arabitol producer from a screening of non-conventional yeasts [9] and was the subject of a preliminary study assessing that arabitol production was not growth-associated and occurred during the stationary phase in presence of an excess of glycerol [21].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improved fed-batch processes with Wickerhamomyces anomalus WC 1501 for the production of d-arabitol from pure glycerol

et al. 2022

View full text Add to dashboard Cite

Background d-Arabitol, a five-carbon sugar alcohol, represents a main target of microbial biorefineries aiming to valorize cheap substrates. The yeast Wickerhamomyces anomalus WC 1501 is known to produce arabitol in a glycerol-based nitrogen-limited medium and preliminary fed-batch processes with this yeast were reported to yield 18.0 g/L arabitol. Results Fed-batch fermentations with W. anomalus WC 1501 were optimized using central composite design (CCD). Dissolved oxygen had not a significant effect, while optimum values were found for glycerol concentration (114.5 g/L), pH (5.9), and temperature (32.5 °C), yielding 29 g/L d-arabitol in 160 h, a conversion yield of 0.25 g of arabitol per g of consumed glycerol, and a volumetric productivity of 0.18 g/L/h. CCD optimal conditions were the basis for further improvement, consisting in increasing the cellular density (3✕), applying a constant feeding of glycerol, and increasing temperature during production. The best performing fed-batch fermentations achieved 265 g/L d-arabitol after 325 h, a conversion yield of 0.74 g/g, and a volumetric productivity of 0.82 g/L/h. Conclusion W. anomalus WC 1501 confirmed as an excellent producer of d-arabitol, exhibiting a remarkable capability of transforming pure glycerol. The study reports among the highest values ever reported for microbial transformation of glycerol into d-arabitol, in terms of arabitol titer, conversion yield, and productivity. Graphical Abstract

show abstract

Section: Table 3 Anova Results For the Quadratic Models Describing Ar...supporting

confidence: 88%

Section: Further Improvements Of the Fed Batch Processessupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved fed-batch processes with Wickerhamomyces anomalus WC 1501 for the production of d-arabitol from pure glycerol

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Under P deficient conditions, there is an increase in l -xylulose, a compound that utilizes carbon as a source for ethanol production, promoting the formation of young shoots under shaded conditions. Arabitol is important for enabling sugar utilization in microorganisms [ 36 ]. l -arabinose is a polymer of cell wall saccharides in plants [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Interactions between Phosphorus and Light Intensity on Metabolite Compositions in Tea Cultivar Longjing43

Long

Zhang

et al. 2022

IJMS

View full text Add to dashboard Cite

Light intensity influences energy production by increasing photosynthetic carbon, while phosphorus plays an important role in forming the complex nucleic acid structure for the regulation of protein synthesis. These two factors contribute to gene expression, metabolism, and plant growth regulation. In particular, shading is an effective agronomic practice and is widely used to improve the quality of green tea. Genotypic differences between tea cultivars have been observed as a metabolic response to phosphorus deficiency. However, little is known about how the phosphorus supply mediates the effect of shading on metabolites and how plant cultivar gene expression affects green tea quality. We elucidated the responses of the green tea cultivar Longjing43 under three light intensity levels and two levels of phosphorus supply based on a metabolomic analysis by GC×GC-TOF/MS (Two-dimensional Gas Chromatography coupled to Time-of-Flight Mass Spectrometry) and UPLC-Q-TOF/MS (Ultra-Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry), a targeted analysis by HPLC (High Performance Liquid Chromatography), and a gene expression analysis by qRT-PCR. In young shoots, the phosphorus concentration increased in line with the phosphate supply, and elevated light intensities were positively correlated with catechins, especially with epigallocatechin of Longjing43. Moreover, when the phosphorus concentration was sufficient, total amino acids in young shoots were enhanced by moderate shading which did not occur under phosphorus deprivation. By metabolomic analysis, phenylalanine, tyrosine, and tryptophan biosynthesis (PTT) were enriched due to light and phosphorus effects. Under shaded conditions, SPX2 (Pi transport, stress, sensing, and signaling), SWEET3 (bidirectional sugar transporter), AAP (amino acid permeases), and GSTb (glutathione S-transferase b) shared the same analogous correlations with primary and secondary metabolite pathways. Taken together, phosphorus status is a crucial factor when shading is applied to increase green tea quality.

show abstract

“…By conducting molecular docking with different substrates closely linked residues were identified. Furthermore, considering the safety of the strain [ 38 ], we used the Bacillus subtilis as the host to express Bp-DAE and the recombinant Bacillus subtilis cells were employed for the biosynthesis of D-allulose from D-fructose. The experimental results elaborate that Bp-DAE could transform D-fructose into D-allulose which could be used to reduce the cost of downstream industries.…”

Section: Introductionmentioning

confidence: 99%

The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production

Tang

Iqbal

et al. 2022

Foods

View full text Add to dashboard Cite

D-allulose is a natural rare sugar with important physiological properties that is used in food, health care items, and even the pharmaceutical industry. In the current study, a novel D-allulose 3-epimerase gene (Bp-DAE) from the probiotic strain Blautia produca was discovered for the production and characterization of an enzyme known as Bp-DAE that can epimerize D-fructose into D-allulose. Bp-DAE was strictly dependent on metals (Mn2+ and Co2+), and the addition of 1 mM of Mn2+ could enhance the half-life of Bp-DAE at 55 °C from 60 to 180 min. It exhibited optimal activity in a pH of 8 and 55 °C, and the Km values of Bp-DAE for the different substrates D-fructose and D-allulose were 235.7 and 150.7 mM, respectively. Bp-DAE was used for the transformation from 500 g/L D-fructose to 150 g/L D-allulose and exhibited a 30% of conversion yield during biotransformation. Furthermore, it was possible to employ the food-grade microbial species Bacillus subtilis for the production of D-allulose using a technique of whole-cell catalysis to circumvent the laborious process of enzyme purification and to obtain a more stable biocatalyst. This method also yields a 30% conversion yield.

show abstract

Microbial hosts for production of D-arabitol: Current state-of-art and future prospects

Cited by 23 publications

References 70 publications

Improved fed-batch processes with Wickerhamomyces anomalus WC 1501 for the production of d-arabitol from pure glycerol

Improved fed-batch processes with Wickerhamomyces anomalus WC 1501 for the production of d-arabitol from pure glycerol

Effect of Interactions between Phosphorus and Light Intensity on Metabolite Compositions in Tea Cultivar Longjing43

The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production

Contact Info

Product

Resources

About