Microbial fuel cell performance improvement based on FliC-deficient E. coli strain

Nguyen, Dang‐Trang; Tamura, Teppei; Tobe, Ryuta; Mihara, Hisaaki; Taguchi, Kozo

doi:10.1016/j.egyr.2020.11.133

Cited by 9 publications

(5 citation statements)

References 10 publications

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“…Similar electricity generation pattern was recorded by Cau et al [2], the highly statistically significant results recorded for S3 samples are in correspondence to that of the TVC results. The isolation of six bacterial strains from the selected sludge sample declared the fact that electrogenic bacteria are present in all ecosystems [38], with different electrogenic potentialities due to their multiple reducing mechanisms [39], primary and rapid identification using VITEK®2 system showed that our isolated bacterial collection included A. salmonicida which is associated with fish [40], S. lentus, E. cloacae, and E. coli which are associated with animals [41], C. testosterone and Pantoea sp which are mainly found in soil, waste water and sludge [42], in agreement with the results recorded by Nguyena et al [43] the most potent electrogenic isolates were E. cloacae, and E. coli, consequently they were subjected to confirmatory molecular characterization by using the 16S rRNA marker gene which has served as an important tool for determining phylogenetic relationships between bacteria. [44].…”

Section: Discussionsupporting

confidence: 90%

Generation of green electricity from sludge using photo-stimulated bacterial consortium as a sustainable technology

Othman,

Ahmed,

Elneklawi

et al. 2023

Microb Cell Fact

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Microbial fuel cell (MFC) is a bio-electrical energy generator that uses respiring microbes to transform organic matter present in sludge into electrical energy. The primary goal of this work was to introduce a new approach to the green electricity generation technology. In this context a total of 6 bacterial isolates were recovered from sludge samples collected from El-Sheikh Zayed water purification plant, Egypt, and screened for their electrogenic potential. The most promising isolates were identified according to 16S rRNA sequencing as Escherichia coli and Enterobacter cloacae, promising results were achieved on using them in consortium at optimized values of pH (7.5), temperature (30°C) and substrate (glucose/pyruvate 1%). Low level red laser (λ = 632.8nm, 8mW) was utilized to promote the electrogenic efficiency of the bacterial consortium, maximum growth was attained at 210 sec exposure interval. In an application of adding standard inoculum (107 cfu/mL) of the photo-stimulated bacterial consortium to sludge based MFC a significant increase in the output potential difference values were recorded, the electricity generation was maintained by regular supply of external substrate. These results demonstrate the future development of the dual role of MFCs in renewable energy production and sludge recycling.

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Section: Discussionsupporting

confidence: 90%

Generation of green electricity from sludge using photo-stimulated bacterial consortium as a sustainable technology

Othman,

Ahmed,

Elneklawi

et al. 2023

Microb Cell Fact

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“…The experimental evidence gathered here opens the doors to explore new applications of E. coli through genetic engineering. For instance, enhancing its electrogenic capacity through the production of endogenous redox mediators [58], direct electron transfer [59], or its ability to form a dense biofilm on the anode surface [20] are strategies that have recently begun to be evaluated. These strategies could further improve the performance of the MFC to achieve large-scale utilization of these devices.…”

Section: Resultsmentioning

confidence: 99%

“…E. coli DH5α is a widely used strain for maintaining and amplifying plasmid DNA [18]. Its effective utilization in microbial cells can facilitate initiatives to expand its application through improvements aimed at achieving a higher efficiency in electron transfer, as has been done recently [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…Recent developments have demonstrated the utility of E. coli in MFC technology, achieving power densities on the order of 11.7 mW m −2 when using E. coli K12 and anodes based on carbon [27]; the use of E. coli DH5 α and anodes based on carbon nanotubes has rendered even larger power densities, reaching 2740 mW m −2 [28,29]. When dealing with E. coli, differences in MFC electron transfer measurements can be explained by electrode materials [15], genetic modifications [30], the use of exogen mediators [20], and cocultures [31,32].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Glucose and Methylene Blue in Microbial Fuel Cells Using E. coli

Montoya-Vallejo,

Gil Posada,

Quintero-Díaz

2023

Energies

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Microbial fuel cells could be used as an alternative for wastewater treatment and electricity generation. Escherichia coli is a representative bacterium that has been widely studied as a model in laboratory assays despite its limited ability to transfer electrons. Although previous studies have employed glucose and methylene blue in electricity production using E. coli, there remains a lack of understanding on how current generation would impact the production of metabolites and what the most appropriate conditions for current production might be. To shed light on those issues, this manuscript used a 32 factorial design to evaluate the effect of the concentration of organic matter (glucose) and the concentration of the mediator methylene blue (MB) using E. coli DH5α as an anodic microorganism. It was found that as the concentration of glucose was increased, the production of electricity increased and at the same time, its degradation percentage decreased. Similarly, a 17-fold increase in current production was observed with an elevation in methylene blue concentration from 0 to 0.3 mM, though inhibition became apparent at higher concentrations. The maximum power generated by the cell was 204.5 µW m−2, achieving a current density of 1.434 mA m−2 at concentrations of 5 g L−1 of glucose and 0.3 mM of MB. Reductions in the production of ethanol, lactate, and acetate were observed due to the deviation of electrons to the anode.

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“…Utilization of food waste can reduce the amount of soil and air pollution, so it is used as a substrate in this study. Microorganisms can produce electric currents are referred to as electrogenic microbes, including Geobacter sulfurreducens [3], Escherichia coli [4], Lactococcus lactis [5], Saccharomyces cerevisiae [6], and Shewanella oneidensis [7]. In this study, the bacteria Shewanella oneidensis will be developed.…”

Section: Introductionmentioning

confidence: 99%

Effect of micronutrient addition and development on microbial fuel cells (MFC) from food waste with the help of hydrolytic fungi

Laily

Juliastuti

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Based on data from the Indonesia State Electricity Company (PLN) in 2021, the electricity capacity distributed throughout Indonesia is still not fulfilled and going to be overcome with renewable energy This research aimed to know the effect of micronutrient addition on the management of a food waste on microbial fuel cells for bioelectricity production. Positive effects of addition of micronutrients on various substrates have been reported, such as addition of Mg2+, Ni2+, Cu2+, Ca2+, Pb2+, Co2+, Cd2+, Cr2+, and Zn2+. First, food waste will be hydrolysed by hydrolityc fungi, then mixed with Shewanella oneidensis MR-1 bacteria, Sidoarjo mud, and 1 µg/L micronutrient. After micronutrient added, observations were made on the power density in 20 days. The best power density production was 6,175,438.596 mW/m2 which achieved by Cobalt addition. The total power density generated with the addition of Cobalt metal ions as a micronutrient was able to reach 29,099,689 mW/m2 or 29,099.69 kW/m2, which could achieve the target electricity power 2,949.58 MW by using a substrate of about 51 tons of food waste with the addition of micronutrient cobalt of about 0.05 gram/L. Thus, MFC can solve electricity problems in Indonesia.

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Microbial fuel cell performance improvement based on FliC-deficient E. coli strain

Cited by 9 publications

References 10 publications

Generation of green electricity from sludge using photo-stimulated bacterial consortium as a sustainable technology

Generation of green electricity from sludge using photo-stimulated bacterial consortium as a sustainable technology

Effect of Glucose and Methylene Blue in Microbial Fuel Cells Using E. coli

Effect of micronutrient addition and development on microbial fuel cells (MFC) from food waste with the help of hydrolytic fungi

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