Microbial Fibrinolytic Enzyme Production and Applications

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

“…With the emergence of "Biocatalysis" as an eco-technology, a promising perspective of microbial fibrinolytic enzymes has progressively attracted therapeutic prominence because of their comprehensive biochemical diversity, low expense, scale-up feasibility and easy genetic modification which could directly lyse existing thrombus inside the blood vessel [9,10]. Scientific reports suggest numerous microbial sources of fibrinolytic enzymes such as bacteria (including actinomycetes), filamentous fungi and microalgae [10,11]. A cultivation-dependent methodology is largely employed for screening microbial producers of fibrinolytic enzymes.…”

“…A cultivation-dependent methodology is largely employed for screening microbial producers of fibrinolytic enzymes. However, with an aim of enhanced production/high efficacy: recombinants, mutagens and metagenomic libraries are also created as cultivable microbes and are no more than 1% of the entire microbial community [10]. Furthermore, different traditional fermented foods are also testified for producing efficient fibrinolytic enzymes, for example: nattokinase extracted from Japanese natto [12,13] and fibrinolytic enzyme [14] from soyabean fermented Douchi efficiently lysed thrombi in vitro and in vivo.…”