Microbial Electroreduction: Screening for New Cathodic Biocatalysts

Rodrigues, Tatiana de Campos; Rosenbaum, Miriam A.

doi:10.1002/celc.201402239

Cited by 51 publications

(26 citation statements)

References 61 publications

(42 reference statements)

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“…Open squares are the corresponding current efficiencies Current densities reported for unbalanced fermentations are in the range of 55 μA/cm 2 for a S. oneidensis based microbial fuel cell in a modified bioreactor (Rosa et al, 2016). For cathodic processes where current is consumed and acetate or butyrate is produced using pure or mixed cultures, current densities range from several μA/cm 2 up to 3.7 mA/cm 2 (de Campos-Rodrigues & Rosenbaum, 2014;Ganigué, Puig, Batlle-Vilanova, Dolors Balaguer, & Colprim, 2015;Giddings, Nevin, Woodward, Lovley, & Butler, 2015;Jourdin et al, 2014). However, a Pseudomonas putida based anodic respiration process using an external mediator showed higher current densities (up to 12 mA/cm 2 ), where overpotentials at the electrodes play a greater role compared to the processes described before and need to be accounted for the electrochemical measurements (Hintermayer et al, 2016).…”

Section: Evaluation Of Electrochemical Losses In the Systemmentioning

confidence: 99%

Characterization of a membrane‐separated and a membrane‐less electrobioreactor for bioelectrochemical syntheses

Krieg

Phan

Wood

et al. 2018

Biotech & Bioengineering

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Bioelectrochemical systems (BESs) have the potential to contribute to the energy revolution driven by the new bio-economy. Until recently, simple reactor designs with minimal process analytics have been used. In recent years, assemblies to host electrodes in bioreactors have been developed resulting in so-called "electrobioreactors." Bioreactors are scalable, well-mixed, controlled, and therefore widely used in biotechnology and adding an electrode extends the possibilities to investigate bioelectrochemical production processes in a standard system. In this work, two assemblies enabling a separated and non-separated electrochemical operation, respectively, are designed and extensively characterized. Electrochemical losses over the electrolyte and the membrane were comparable to H-cells, the bioelectrochemical standard reaction system. An effect of the electrochemical measurements on pH measurements was observed if the potential is outside the range of -1,000 to +600 mV versus Ag/AgCl. Electrobiotechnological characterization of the two assemblies was done using Shewanella oneidensis as an electroactive model organism. Current production over time was improved by a separation of anodic and cathodic chamber by a Nafion® membrane. The developed electrobioreactor was used for a scale-up of the anaerobic bioelectrochemical production of organic acids and lysine from glucose using an engineered Corynebacterium glutamicum. Comparison to a small-scale custom-made electrobioreactor indicates that anodic electro-fermentation of lysine and organic acids might not be limited by the BES setup but by the biocatalysis of the cells.

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Section: Evaluation Of Electrochemical Losses In the Systemmentioning

confidence: 99%

Characterization of a membrane‐separated and a membrane‐less electrobioreactor for bioelectrochemical syntheses

Krieg

Phan

Wood

et al. 2018

Biotech & Bioengineering

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“…Large efforts have been done to optimize known microbial catalysts for MES and to screen new ones with strong electroautotrophic properties (Rodrigues and Rosenbaum, 2014). Meanwhile, cathodes fabricated with novel materials or designed with better spatial arrangement are being explored and developed.…”

Section: Discussionmentioning

confidence: 99%

“…Direct electron transfer from the cathode to the microbial catalyst in the absence of a redox mediator has been shown to occur in MES processes driven by the following electroautotrophic species: A. ferrooxidans (Carbajosa et al, 2010; Rodrigues and Rosenbaum, 2014), M. ferrooxydans (Summers et al, 2013) and a group of acetogenic bacteria (Nevin et al, 2010, 2011; Nie et al, 2013; Zhang et al, 2013). This conclusion is mainly based on the fact that all these MES systems were operated with cathodes poised at potentials too high to produce significant quantity of H 2 .…”

Section: Electron Transfer From the Cathode To The Microbial Catalystmentioning

confidence: 99%

Electrifying microbes for the production of chemicals

2015

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Powering microbes with electrical energy to produce valuable chemicals such as biofuels has recently gained traction as a biosustainable strategy to reduce our dependence on oil. Microbial electrosynthesis (MES) is one of the bioelectrochemical approaches developed in the last decade that could have critical impact on the current methods of chemical synthesis. MES is a process in which electroautotrophic microbes use electrical current as electron source to reduce CO2 to multicarbon organics. Electricity necessary for MES can be harvested from renewable resources such as solar energy, wind turbine, or wastewater treatment processes. The net outcome is that renewable energy is stored in the covalent bonds of organic compounds synthesized from greenhouse gas. This review will discuss the future of MES and the challenges that lie ahead for its development into a mature technology.

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“…Several acetogenic bacteria, including Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Sporumosa acidovorans, Sporumosa malonica, Clostridium ljungdahlii, Clostridium aceticum, and Moorella thermoacetica, can utilize the cathodic current for CO 2 reduction to organic acids (Nevin et al, 2011;Aryal et al, 2017). Also, some autotrophic sulfate-reducing microorganisms (SRM) have shown the ability to consume electrons from the cathode to accomplish sulfate reduction and hydrogen (H 2 ) production (Rodrigues and Rosenbaum, 2014;Beese-Vasbender et al, 2015b). However, overall fairly little research has been devoted toward this last group of electroautotrophic biocatalysts.…”

Section: Introductionmentioning

confidence: 99%

Sulfate-Reducing ElectroAutotrophs and Their Applications in Bioelectrochemical Systems

Agostino

Rosenbaum

2018

Front. Energy Res.

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Electroautotrophs are microbes able to perform different biocathodic reactions by using CO 2 as sole carbon source and electrochemical reducing power as a sole energy source. Electroautotrophy has been discovered in several groups of microorganisms, including iron-oxidizing bacteria, iron-reducing bacteria, nitrate-reducing bacteria, acetogens, methanogens and sulfate-reducing bacteria. The high diversity of electroautrophs results in a wide range of Bioelectrochemical Systems (BES) applications, ranging from bioproduction to bioremediation. In the last decade, particular research attention has been devoted toward the discovery, characterization and application of acetogenic and methanogenic electroautotrophs. Less attention has been given to autotrophic sulfate-reducing microorganisms, which are extremely interesting biocatalysts for multiple BES technologies, with concomitant CO 2 fixation. They can accomplish water sulfate removal, hydrogen production and, in some case, even biochemicals production. This mini-review gives a journey into electroautotrophic ability of sulfate-reducing bacteria and highlights their possible importance for biosustainable applications. More specifically, general metabolic features of autotrophic sulfate reducers are introduced. Recently discovered strains able to perform extracellular electron uptake and possible molecular mechanisms behind this electron transfer capacity are explored. Finally, BES technologies based on sulfate-reducing electroautotrophs are illustrated.

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Microbial Electroreduction: Screening for New Cathodic Biocatalysts

Cited by 51 publications

References 61 publications

Characterization of a membrane‐separated and a membrane‐less electrobioreactor for bioelectrochemical syntheses

Characterization of a membrane‐separated and a membrane‐less electrobioreactor for bioelectrochemical syntheses

Electrifying microbes for the production of chemicals

Sulfate-Reducing ElectroAutotrophs and Their Applications in Bioelectrochemical Systems

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