Microbial ecosystem and fermentation traits in the caecum of growing rabbits given diets varying in neutral detergent soluble and insoluble fibre levels

Rodríguez-Romero, N.; Abecia, Leticia; Fondevila, M.

doi:10.1016/j.anaerobe.2013.02.001

Cited by 7 publications

(7 citation statements)

References 41 publications

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“…Previous studies focused particularly on the analysis of the effects of the dietary fiber level on the whole cecal bacterial community in rabbits using molecular profiles (Michelland et al, 2010Monteils et al, 2008;Rodríguez-Romero, Abecia, & Fondevila, 2013). Our study showed that the cecal microbiota abundance increased significantly when NDF levels reached 350-400 g/kg (original matter basis) (p < 0.001).…”

Section: Discussionmentioning

confidence: 60%

“…Although the rabbit relies on the intake of large quantities of fiber that can be fermented by the microbiota found predominantly within the cecum, the change in NDF level does not affect the cecal pH and VFA concentration, which indicates no relation between quantity of NDF and concentration of VFA in the cecum (Bellier & Gidenne, ). Previous studies focused particularly on the analysis of the effects of the dietary fiber level on the whole cecal bacterial community in rabbits using molecular profiles (Michelland et al., , ; Monteils et al., ; Rodríguez‐Romero, Abecia, & Fondevila, ). Our study showed that the cecal microbiota abundance increased significantly when NDF levels reached 350–400 g/kg (original matter basis) ( p < 0.001).…”

Section: Discussionmentioning

confidence: 99%

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Effect of dietary fiber levels on bacterial composition with age in the cecum of meat rabbits

Zhou

et al. 2018

MicrobiologyOpen

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This study investigated the influence of dietary fiber levels on the growth performance, digestion, metabolism, and cecal microbial community of rabbits with different diets at different age. The different levels of dietary natural detergent fiber (NDF) were formulated accordingly: 400(A), 350(B), 300(C), 250(D) g/kg original matter basis, respectively; the different ages were 52, 62, and 72 days. With NDF increasing, the average daily feed intake (ADFI) and feed conversion rate (FCR) were increased, whereas average daily gain (ADG) and mortality were decreased (p < 0.05). The stomach relative weight, stomach content relative weight, cecal relative weight, and cecal content weight increased with increasing NDF (p < 0.05). The NH -N concentration of cecum dropped when the dietary NDF increased (p < 0.05). The diversity of the total microbiota increased significantly in Diets B, C (p = 0.011), and reached the lowest in 52 days for all diet groups. The richness index was decreased significantly in Diet A, D (p < 0.05) and in 62 days (p < 0.001), respectively. The phylum Firmicutes was higher (p < 0.01) in rabbits fed Diets B, C than Diets A, D and Bacteroidetes was highest in Diets C, D, and Proteobacteria was the highest in Diet A (p < 0.001). Among the classified genera, there were 14 that had levels of abundance of more than 1% and were commonly shared by all samples. Ruminococcus spp. that produced volatile fatty acid (VFA) abundance was highest from Diets B, C at 52 and 62 days. It is interesting to note that Bifidobacterium from Diet C was the most abundant genus during the entire experimental period (p < 0.01). The data from Venn diagrams, principal component analysis (PCA), and heat map plots of the bacterial communities showed that there were more groups of shared microbiota with aging. The above results indicate the cecal microbiota controlled by the 350 g/kg NDF diet can prevent gastrointestinal distress and exhibit good production performance.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Effect of dietary fiber levels on bacterial composition with age in the cecum of meat rabbits

Zhou

et al. 2018

MicrobiologyOpen

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“…Thus, differences in Jaccard but not Yue & Clayton indices suggest that changes in the community membership (addition or loss of members) occurred, but that this may have mainly involved low abundance members of community based on a lack of difference when relative abundance is considered. Other than the potential long-term microbiota adjustment to a new diet [42] the accumulated effect of meloxicam should also be considered, and in the absence of clinical or histologic abnormalities, only advanced molecular testing methods had the sensitivity to detect subtle changes in the rabbit’s GIT microbiota.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, it is known that the fecal microbiome of laboratory raised rabbits is different from rabbits raised under other conditions (e.g. farms, households) [42-44]. As with similar studies, there is always the question of how well the fecal microbiome represents the microbiome in more proximal sections of the gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of the microbiota in hard feces from healthy rabbits (Oryctolagus cuniculus) medicated with long term oral meloxicam

Eshar

Weese

2014

BMC Vet Res

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BackgroundAnalgesia is often indicated in rabbits undergoing surgical procedures or suffering from various painful conditions and the most common adverse effects associated with NSAIDs occur in the gastrointestinal tract (GIT). The objective of this study was to determine the potential effect of long-term (21 days) meloxicam administration on the fecal bacterial microbiota in healthy rabbits.Samples of hard feces were collected from six rabbits treated with meloxicam (1 mg/kg orally once every 24 h) on days 0,6,14 and 21. Next generation sequencing of V4 16S rRNA gene products was performed.ResultsA total of 2589912 V4 rRNA gene sequences passed all quality control filters. Firmicutes predominated (82.0 ± 6.2%). Sixteen other phyla were also identified but other than Verrucomicrobia (4.4 ± 4.9%), all accounted for less than 1% of the identified sequences. Within Firmicutes, Clostridia was the dominant class, accounting for 76% of operational taxon units (OTUs). In general, there were only few differences observed between time points and different rabbits at the phylum level. A significant change was observed in the relative abundance of Proteobacteria over the 4 time points (P = 0.02).ConclusionsThe gastrointestinal tract of rabbits harbors dense and diverse microbiota. Significant alteration of the hard fecal microbiota does not appear to be a considerable adverse effect expected in rabbits treated for 21 days with oral meloxicam at a dose of 1 mg/kg.

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“…Furthermore, Badiola et al (2016) observed that the inoculation of rabbit kits with B. dorei reduced the expression of pro-inflammatory cytokines and increased the expression of MHC II, a surface antigen, as well as increasing immunoglobulin diversity, thereby possibly helping reduce the risk of ERE. The prevalence of the genus Ruminococcus concurred with Bäuerl et al (2014), and R. albus, the most abundant species identified in this genus and 4 th most abundant overall, is generally recognised as being one of the main cellulolytic bacteria in the rumen environment and has been previously reported in the rabbit caecum (Abecia et al, 2005;Monteils et al, 2008;Rodríguez-Romero et al, 2013). In contrast, R. gnavus, the fifth most abundant species, does not appear to have been previously detected in rabbits.…”

Section: The Microbiota Profilementioning

confidence: 61%

Composition of rabbit caecal microbiota and the effects of dietary quercetin supplementation and sex thereupon

2019

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The purpose of this study was to add to the current understanding of rabbit caecal microbiota. This involved describing its microbial composition and linking this to live performance parameters, as well as determining the effects of dietary quercetin (Qrc) supplementation (2 g/kg feed) and sex on the microbial population. The weight gain and feed conversion ratio of twelve New Zealand White rabbits was measured from 5 to 12 wk old, blood was sampled at 11 wk old for the determination of serum hormone levels, and the rabbits were slaughtered and caecal samples collected at 13 wk old. Ion 16STM metagenome sequencing was used to determine the microbiota profile. The dominance of Firmicutes (72.01±1.14% of mapped reads), Lachnospiraceae (23.94±1.01%) and Ruminococcaceae (19.71±1.07%) concurred with previous reports, but variation both between studies and individual rabbits was apparent beyond this. Significant correlations between microbial families and live performance parameters were found, suggesting that further research into the mechanisms of these associations could be useful. Negative correlations with the caecal flavonoid content were found, but the latter was not affected by diet, and the effects of quercetin supplementation on the microbiota were very limited, possibly due to the absorption of the quercetin-aglycone from the gastrointestinal tract prior to the caecum. Nonetheless, Clostridiales Family XIII. Incertae Sedis was more abundant in the quercetin-supplemented rabbits (Control: 0.003±0.003%; Qrc: 0.020±0.000; P=0.005), as was the genus Anaerofustis (Control: 0.000±0.002; Qrc: 0.010±0.002; P=0.003). Serum cortisol levels were higher in females, and several microbial families differed between the sexes. Most were more abundant in female rabbits, including the most abundant, the family Eubacteriaceae (Male: 2.93±0.40; Female: 4.73±0.40; P=0.01).

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Microbial ecosystem and fermentation traits in the caecum of growing rabbits given diets varying in neutral detergent soluble and insoluble fibre levels

Cited by 7 publications

References 41 publications

Effect of dietary fiber levels on bacterial composition with age in the cecum of meat rabbits

Effect of dietary fiber levels on bacterial composition with age in the cecum of meat rabbits

Molecular analysis of the microbiota in hard feces from healthy rabbits (Oryctolagus cuniculus) medicated with long term oral meloxicam

Composition of rabbit caecal microbiota and the effects of dietary quercetin supplementation and sex thereupon

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