Microbial ecology of a perchlorate-reducing, hydrogen-based membrane biofilm reactor

Nerenberg, Robert; Kawagoshi, Yasunori; Rittmann, Bruce E.

doi:10.1016/j.watres.2007.08.033

Cited by 84 publications

(62 citation statements)

References 35 publications

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“…The membrane biofilm reactor (MBfR), a new technical approach for bio-reduction of oxidized contaminants (Ergas and Reuss, 2001;Lee and Rittmann, 2002;Nerenberg and Rittmann, 2004;Nerenberg et al, 2003;Rittmann et al, 2004), delivers hydrogen gas (H 2 ) as the electron donor. H 2 is non-toxic, leaves no residuals that could cause bacterial regrowth, and is a biologically available electron donor for the reduction of many oxidized contaminants Rittmann et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Bio‐reductive dechlorination of 1,1,1‐trichloroethane and chloroform using a hydrogen‐based membrane biofilm reactor

Chung

Rittmann

2006

Biotech & Bioengineering

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A H(2)-based, denitrifying and sulfate-reducing membrane biofilm reactor (MBfR) was effective for removing 1,1,1-trichloroethane (TCA) and chloroform (CF) by reductive dechlorination. When either TCA or CF was first added to the MBfR, reductive dechlorination took place immediately and then increased over 3 weeks, suggesting enrichment for TCA- or CF-dechlorinating bacteria. Increasing the H(2) pressure increased the dechlorination rates of TCA or CF, and it also increased the rate of sulfate reduction. Increased sulfate loading allowed more sulfate reduction, and this competed with reductive dechlorination, particularly the second steps. The acceptor flux normalized by effluent concentration can be an efficient indicator to gauge the intrinsic kinetics of the MBfR biofilms for the different reduction reactions. The analysis of normalized rates showed that the kinetics for reductive-dechlorination reactions were slowed by reduced H(2) bio-availability caused by a low H(2) pressure or competition from sulfate reduction.

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Section: Introductionmentioning

confidence: 99%

Bio‐reductive dechlorination of 1,1,1‐trichloroethane and chloroform using a hydrogen‐based membrane biofilm reactor

Chung

Rittmann

2006

Biotech & Bioengineering

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“…These concerns can be addressed only by expanding efforts to characterize the structures and activities of the microbial communities responsible for biological drinking water treatment and to link this information to the microbiological properties of finished drinking water. A few studies already have characterized microbial communities in drinking water treatment systems by using phospholipid fatty acids (32), culture-based methods (31,38), and nucleic acid-based methods (18,33,41). More work is needed to elucidate how operational conditions, such as nutrient additions, influence microbial community composition and function to help drinking water utilities and regulators evaluate this emerging technology.…”

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confidence: 99%

Changes in the Structure and Function of Microbial Communities in Drinking Water Treatment Bioreactors upon Addition of Phosphorus

Upadhyaya

Yuen

et al. 2010

Appl Environ Microbiol

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Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically different responses in microbial community structure were detected. Improvement in reactor performance with respect to perchlorate and nitrate removal started within a few days after phosphorus addition for both reactors. Microbial community structures were evaluated using molecular techniques targeting 16S rRNA genes. Clone library results showed that the relative abundance of perchlorate-reducing bacteria (PRB) Dechloromonas and Azospira in the bench-scale reactor increased from 15.2% and 0.6% to 54.2% and 11.7% after phosphorus addition, respectively. Real-time quantitative PCR (qPCR) experiments revealed that these increases started within a few days after phosphorus addition. In contrast, after phosphorus addition, the relative abundance of Dechloromonas in the pilot-scale reactor decreased from 7.1 to 0.6%, while Zoogloea increased from 17.9 to 52.0%. The results of this study demonstrated that similar operating conditions for bench-scale and pilot-scale reactors resulted in similar contaminant removal performances, despite dramatically different responses from microbial communities. These findings suggest that it is important to evaluate the microbial community compositions inside bioreactors used for drinking water treatment, as they determine the microbial composition in the effluent and impact downstream treatment requirements for drinking water production. This information could be particularly relevant to drinking water safety, if pathogens or disinfectant-resistant bacteria are detected in the bioreactors.

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“…However, during this 15 day period, both NO À 3 and NO À 2 were detected at 5 and 11 mg N/L, respectively, which may suggest that SeO 2À 4 was secondarily reduced to low levels with NO À 3 as the main electron acceptor for growth. Similarly, Nerenberg and Rittmann (2002) and Nerenberg et al (2008) concluded that perchlorate (ClO 4 À ) could be secondarily reduced with NO À 3 as the primary acceptor for growth. On an electron equivalent basis, at the influent concentrations during Phase 1, NO À 3 reduction would consume 65 times more electrons than SeO 2À 4 reduction.…”

Section: Performance During Phases 1-3 the Treatment Of Synthetic Fgmentioning

confidence: 97%

The removal of selenate to low ppb levels from flue gas desulfurization brine using the H2-based membrane biofilm reactor (MBfR)

Ginkel

Yang

Kim

et al. 2011

Bioresource Technology

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Microbial ecology of a perchlorate-reducing, hydrogen-based membrane biofilm reactor

Cited by 84 publications

References 35 publications

Bio‐reductive dechlorination of 1,1,1‐trichloroethane and chloroform using a hydrogen‐based membrane biofilm reactor

Bio‐reductive dechlorination of 1,1,1‐trichloroethane and chloroform using a hydrogen‐based membrane biofilm reactor

Changes in the Structure and Function of Microbial Communities in Drinking Water Treatment Bioreactors upon Addition of Phosphorus

The removal of selenate to low ppb levels from flue gas desulfurization brine using the H2-based membrane biofilm reactor (MBfR)

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