Microbial diversity structure in acetate single chamber microbial fuel cell for electricity generation

Abstract

This study investigates the performance of acetate feed membrane less single chamber microbial fuel cell and physical characterization of the bio film present on the anode surface using Scanning Electron Microscope (SEM) and 16S rRNA analyzer. The performance has been investigated using Teflon treated carbon paper with 0.3 mg/cm2 Pt/C loaded as a cathode and carbon paper as an anode. The maximum open circuit potential is noticed as 791 mV, the system successfully revealed a maximum power density of 86.1 mW m−2 at stable current density of 354 mA m−2 with high coulombic efficiency of 65% at maximum degradation rate of 96%. SEM showed the dense adherence of microorganisms on the anode. 16S rRNA sequencing results indicates phylogenetic mixture in the communities of anodic biofilm and there is no single dominant bacterial species. The dominant phyla are Firmicutes, Gamma Proteobacteria, Alpha Proteobacteria, Actinobacteria, with ten dominant microbial strains: Bacillus firmus, Shewanella profunda, Bacillus isronensis, Brevundimonas bullata, Pseudomonas putida, Planococcus citreus, Micrococcus endophyticus, Acinetobacter tandoii, Bacillus safensis and Shewanella xiamenensis.

Overview of Recent Advancements in the Microbial Fuel Cell from Fundamentals to Applications: Design, Major Elements, and Scalability

“…Technically, microorganisms are ideal catalysts and self-regenerating systems that can produce Energies 2019, 12, 3390 6 of 20 electrical energy from a variety of renewable chemical sources. These chemicals are the carbon source for bacteria in MFCs and can be either simple carbohydrate sources, such as sucrose, glucose [32], acetate [33], alcohols [34], or grape juice [35]; or complex carbon sources such as wastewaters [36], starch [27], or the sludge from chocolate industries [37], food processing [38], beer breweries [39], and sewage [40]; and food industry wastes [41]. As a result of microorganisms' flexibility in consuming a broad range of fuels derived from waste, the MFC is considered globally to represent an ideal technology for bioelectricity generation from renewable biomass.…”

Evolutionary dynamics of microbial communities in bioelectrochemical systems

“…Previous studies analyzed changes within microbial populations for up to several weeks (reviewed in Daghio et al, 2015;Philips et al, 2015;Khater et al, 2017), and employed mainly 16S sequencing (Ishii et al, 2013;Ishii et al, 2014;Dennis et al, 2016), Ribosomal Intergenic Spacer Analysis (RISA) (Paitier et al, 2017), or Denaturing Gradient Gel Electrophoresis (DGGE) (Beecroft et al, 2012). In another study, community shifts were tracked during 90-days of operation (Beecroft et al, 2012).…”

Improvement of the bioelectrochemical hydrogen production from food waste fermentation effluent using a novel start‐up strategy

“…There were no apparent putative nanowires, pili, or other appendages evident in the images. Exoelectrogenic bacteria are connected and added to the surface of the anode, forming a living matrix of protein and sugar . The anodic electroactive biofilm was dominated by flagellated and rod microorganisms that are related to the morphology of Geobacter spp.…”

“…Exoelectrogenic bacteria are connected and added to the surface of the anode, forming a living matrix of protein and sugar. 35 The anodic electroactive biofilm was dominated by flagellated and rod microorganisms that are related to the morphology of Geobacter spp. This morphology has been reported for members of Geobacteraceae, which are gram-negative, nonspore-forming rods with rounded ends.…”