Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor

Date, Yasuhiro; Isaka, Kazuichi; Ikuta, Hajime; Sumino, Tatsuo; Kaneko, N.; Yoshie, Sachiko; Tsuneda, Satoshi; Inamori, Yuhei

doi:10.1016/j.jbiosc.2008.11.015

Cited by 47 publications

(15 citation statements)

References 17 publications

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“…The sequences grouped into C. Brocadia anammoxidans subcluster were similar to the sequences recovered from an anaerobic continuous‐feeding cultivation reactor (Date et al . ), while the sequences grouped into the C. Brocadia flugida subcluster were affiliated to sequences discovered from Cape Fear River and Jiaojiang Estuary (Hirsch et al . ; Hu et al .…”

Section: Resultsmentioning

confidence: 61%

Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong

Sun

et al. 2013

J Appl Microbiol

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Aims: The aim of this study was to characterize anaerobic ammonium oxidation (anammox) community in sediments of the Dongjiang River, a drinking water source of Hong Kong. Methods and Results: The diversity and distribution of the anammox community were investigated based on a comparative analyses of 16S rRNA and hydrazine oxidation (hzo) genes of anammox bacteria. Candidatus Brocadia and two new anammox bacterial clusters were detected based on phylogenetic analysis of 16S rRNA genes. In contrast, the targeting of hzo genes indicated the presence of only Candidatus Jettenia with four different clusters. It was found that the sequence diversities of hzo genes were higher than those of the 16S rRNA genes. The abundance of anammox bacteria varied significantly among the sediment samples based on qPCR. Pearson correlation analysis indicated that nitrite concentration was the key factor influencing the abundance of anammox bacteria. The redundance analysis (RDA) confirmed that the combination of the contents of nitrite and nitrate, and the ratio of total nitrogen vs total carbon (TN/TC) had significant impact on the anammox bacterial community structure. Conclusions:The results revealed that the diverse anammox bacteria were present in sediments of the Dongjiang River, and the community structures were associated with varied environmental factors caused by urban pollutant invasion. Significance and Impact of the Study: This is the first report about the distribution of anammox bacterial community in sediments of the Dongjiang River, which provides helpful information of anammox niche specificity and influencing factors in the river ecosystem.

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Section: Resultsmentioning

confidence: 61%

Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong

Sun

et al. 2013

J Appl Microbiol

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“…Brocadia genus) in similarly operated anammox reactors. [14] This discrepancy may be linked to (i) the fact that these authors did not investigate the anammox diversity in inocula (therefore, we do not know if the anammox diversity was similar or different among the three inocula), (ii) the methods used to assess the microbial community diversity (in the present study, we used 454-pyrosequencing technology and the previous study used 16S rRNA gene sequencing), (iii) the type of seed sludge (these authors used nitrifying, sewage, and digester sludges, while we used sediments from a wetland and from an oil-refinery WWTP).…”

Section: Anammox Organism Detected By 454-pyrosequencingmentioning

confidence: 99%

“…Among these phyla frequently described in the anammox reactors, Downloaded by [Erciyes University] at 14:00 26 December 2014 This study a 16S rDNA fragments were amplified with PCR performed using the primers Pla46F-1390R, except in five studies [11,14,16,47,54] in which universal bacterial primers, that is, 6F-1510R, 11F-1387R, 27F-1492R, 341f-926r, respectively, were used. b This result was a comparative analysis of all 16S rRNA gene sequences retrieved from metagenome sequencing.…”

Section: The Denitrifiersmentioning

confidence: 99%

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Impact of inocula and operating conditions on the microbial community structure of two anammox reactors

Costa

Carvalho

Leal

et al. 2014

Environmental Technology

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The microbial community structure of the biomass selected in two distinctly inoculated anaerobic oxidation of ammonium (anammox) reactors was investigated and compared with the help of data obtained from 454-pyrosequencing analyses. The anammox reactors were operated for 550 days and seeded with different sludges: sediment from a constructed wetland (reactor I) and biomass from an aerated lagoon part of the oil-refinery wastewater treatment plant (reactor II). The anammox diversity in the inocula was evaluated by 16S rRNA gene-cloning analysis. The diversity of anammox bacteria was greater in the sludge from the oil-refinery (three of the five known genera of anammox were detected) than in the wetland sludge, in which only Candidatus Brocadia was observed. Pyrosequencing analysis demonstrated that the community enriched in both reactors had differing compositions despite the nearly similar operational conditions applied. The dominant phyla detected in both reactors were Proteobacteria, Chloroflexi, Planctomycetes, and Acidobacteria. The phylum Bacteroidetes, which is frequently observed in anammox reactors, was not detected. However, Acidobacteria and GN04 phyla were observed for the first time, suggesting their importance for this process. Our results suggest that, under similar operational conditions, anammox populations (Ca. Brocadia sinica and Ca. Brocadia sp. 40) were selected in both reactors despite the differences between the two initial inocula. Taken together, these results indicated that the type of inoculum and the culture conditions are key determinants of the general microbial composition of the biomass produced in the reactors. Operational conditions alone might play an important role in anammox selection.

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“…Anammox bacteria, being strictly anaerobic and autotrophic, are difficult to enrich making application of this process limited due to unavailability of sufficient biomass required for the process. Different methods have been employed to culture and enrich anammox biomass from different types of seed sludge [51, 52]. A relative population of 88% anammox bacteria was achieved in a batch study inoculated from a rotating biological contactor (RBC) treating a landfill leachate [53].…”

Section: Anammox Culture In the Laboratorymentioning

confidence: 99%

Anaerobic Ammonium Oxidation: From Laboratory to Full-Scale Application

Zhang

2013

BioMed Research International

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From discovery in the early 1990s to completion of full-scale anammox reactor, it took almost two decades to uncover the secret veil of anammox bacteria. There were three milestones during the commercialization of anammox: the development of the first enrichment culture medium, the completion of the first commercial anammox reactor, and the fast start-up of full-scale anammox plant. Till now, the culture of anammox bacteria experienced a big progress through two general strategies: (a) to start up a reactor from scratch and (b) to seed the reactor with enriched anammox sludge. The first full-scale anammox reactor took 3.5 years to realize full operation using the first approach due to several reasons besides the lack of anammox sludge. On the other hand, the first Asian anammox reactor started up in two months, thanks to the availability of anammox seed. Along with the implementation of anammox plants, anammox eventually becomes the priority choice for ammonium wastewater treatment.

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Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor

Cited by 47 publications

References 17 publications

Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong

Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong

Impact of inocula and operating conditions on the microbial community structure of two anammox reactors

Anaerobic Ammonium Oxidation: From Laboratory to Full-Scale Application

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