Microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil Sphenophorus levis (Coleoptera, Curculionidae)

Rinke, Raquel; Costa, Andrés; Fonseca, Fernando P.P.; Almeida, L. C.; Delalibera, Ítalo; Henrique-Silva, Flávio

doi:10.4238/2011.november.1.1

Cited by 25 publications

(19 citation statements)

References 29 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding is in line with previous studies of other insects (Rani et al, 2009;Belda et al, 2011;Rinke et al, 2011;Gumiel et al, 2015). The potential explanation behind this observation is that insects reared under laboratory conditions are not under the same selective pressure as wild specimens.…”

Section: Discussionsupporting

confidence: 92%

Cultivable Bacterial Diversity in the Gut of the Chagas Disease Vector Triatoma dimidiata: Identification of Possible Bacterial Candidates for a Paratransgenesis Approach

et al. 2018

View full text Add to dashboard Cite

Since bacterial symbionts play a vital role in the metabolism of hematophagous insect vectors the method known as paratrangenesis, which consists of the use of cultivable insect symbionts to interfere with the transmission of vector-transmitted pathogens has been shown to be effective in certain vector control oriented studies. In Chagas disease research a recent study introduced transgenes through a parastransgenic approach and prevented the development of a vector species for this disease. However this approach requires a previous characterization of the bacterial symbionts present in the species vector of interest, since a selection of the cultivable bacterial symbiont used is mandatory. In this study, we describe the gut bacterial diversity of Triatoma dimidiata specimens from Southern Mexico. Bacteria from both wild and laboratory-reared specimens were cultured, and resulting colonies were grown individually, harvested, and subsequently identified by 16S ribosomal loci sequencing. A total of five and three genera and a total of nine and six bacterial species were identified from field captured and laboratory reared Triatoma dimidiata specimens respectively. A majority of Gram positive bacteria were identified, which included the genera Staphylococcus, Bacillus, Brevibacterium, Micrococcus, and Delftia. Given previous studies we propose the use of Staphylococcus saprophyticus, Micrococcus luteus, and Bacillus megaterium as potential candidates for future paratransgenic efforts done with Triatoma dimidiata, which is one of the most important vectors of Chagas disease, in Central and South America. Given the vital association bacterial symbionts play in the metabolism of routes of hematophagous insect vectors Paratransgenesis consists of the use of cultivable insect symbionts to interfere with the transmission of vector-transmitted pathogens.

show abstract

Section: Discussionsupporting

confidence: 92%

Cultivable Bacterial Diversity in the Gut of the Chagas Disease Vector Triatoma dimidiata: Identification of Possible Bacterial Candidates for a Paratransgenesis Approach

et al. 2018

View full text Add to dashboard Cite

show abstract

“…4B). The expression pattern in different developmental stages was very similar to a digestive peptidase cathepsin L from Sphenophorus levis (Fonseca et al, 2011).…”

Section: Resultsmentioning

confidence: 83%

A novel β-fructofuranosidase in Coleoptera: Characterization of a β-fructofuranosidase from the sugarcane weevil, Sphenophorus levis

Pedezzi

Fonseca

Júnior

et al. 2014

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…While the Nardonella symbionts have been conserved among diverse weevils and cospeciated with the weevil hosts for over 100 My (23)(24)(25)(26)(27)(28)(29)(30)(31), previous studies have identified a number of weevil lineages in which the Nardonella symbiont had been either lost or replaced by novel bacterial symbionts (23,24,(32)(33)(34)(35). The extremely reduced Nardonella genome down to 0.2 Mb (Fig.…”

Section: Insights Into Symbiont Replacements and Diversification In Ementioning

confidence: 99%

“…(18)(19)(20), of which weevils comprise the most species-rich group, the superfamily Curculionoidea, with some 70,000 described species in the world (19,21,22). Many, if not all, weevils are associated with an ancient γ-proteobacterial endosymbiont lineage, Nardonella, in the bacteriome, whose evolutionary origin is estimated as older than 100 My (23)(24)(25)(26)(27)(28)(29)(30)(31). Despite the longlasting host-symbiont coevolution, Nardonella's biological role has been poorly understood (26).…”

Section: Significancementioning

confidence: 99%

Small genome symbiont underlies cuticle hardness in beetles

Anbutsu

Moriyama

Nikoh

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

127

157

View full text Add to dashboard Cite

Beetles, representing the majority of the insect species diversity, are characterized by thick and hard cuticle, which plays important roles for their environmental adaptation and underpins their inordinate diversity and prosperity. Here, we report a bacterial endosymbiont extremely specialized for sustaining beetle’s cuticle formation. Many weevils are associated with a γ-proteobacterial endosymbiont lineage Nardonella, whose evolutionary origin is estimated as older than 100 million years, but its functional aspect has been elusive. Sequencing of Nardonella genomes from diverse weevils unveiled drastic size reduction to 0.2 Mb, in which minimal complete gene sets for bacterial replication, transcription, and translation were present but almost all of the other metabolic pathway genes were missing. Notably, the only metabolic pathway retained in the Nardonella genomes was the tyrosine synthesis pathway, identifying tyrosine provisioning as Nardonella’s sole biological role. Weevils are armored with hard cuticle, tyrosine is the principal precursor for cuticle formation, and experimental suppression of Nardonella resulted in emergence of reddish and soft weevils with low tyrosine titer, confirming the importance of Nardonella-mediated tyrosine production for host’s cuticle formation and hardening. Notably, Nardonella’s tyrosine synthesis pathway was incomplete, lacking the final step transaminase gene. RNA sequencing identified host’s aminotransferase genes up-regulated in the bacteriome. RNA interference targeting the aminotransferase genes induced reddish and soft weevils with low tyrosine titer, verifying host’s final step regulation of the tyrosine synthesis pathway. Our finding highlights an impressively intimate and focused aspect of the host–symbiont metabolic integrity via streamlined evolution for a single biological function of ecological relevance.

show abstract

Microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil Sphenophorus levis (Coleoptera, Curculionidae)

Cited by 25 publications

References 29 publications

Cultivable Bacterial Diversity in the Gut of the Chagas Disease Vector Triatoma dimidiata: Identification of Possible Bacterial Candidates for a Paratransgenesis Approach

Cultivable Bacterial Diversity in the Gut of the Chagas Disease Vector Triatoma dimidiata: Identification of Possible Bacterial Candidates for a Paratransgenesis Approach

A novel β-fructofuranosidase in Coleoptera: Characterization of a β-fructofuranosidase from the sugarcane weevil, Sphenophorus levis

Small genome symbiont underlies cuticle hardness in beetles

Contact Info

Product

Resources

About