Microbial diversity in innovative mesophilic/thermophilic temperature-phased anaerobic digestion of sludge

Gagliano, Maria Cristina; Braguglia, Camilla Maria; Gallipoli, Agata; Gianico, Andrea; Rossetti, Simona

doi:10.1007/s11356-014-3061-y

Cited by 37 publications

(12 citation statements)

References 44 publications

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“…The biomass at 45 °C was slightly lower in terms of diversity and richness than that of the sludge . However, the values of Chao1 and Shannon indices were higher for the 45 °C digester than the data reported previously . The rarefaction curves of all the observed OTUs persistently increased with the number of sequences and reached a plateau, demonstrating that the number of sequences was high enough to cover the diverse microbial species in each sample.…”

Section: Discussionmentioning

confidence: 49%

“…48 digester than the data reported previously. 49,50 The rarefaction curves of all the observed OTUs persistently increased with the number of sequences and reached a plateau, demonstrating that the number of sequences was high enough to cover the diverse microbial species in each sample. The biomass retrieved at 45 ∘ C was the steepest, reflecting the highest species richness among the samples [ Fig.…”

Section: Bacterial Community Composition and Diversitymentioning

confidence: 96%

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Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge

Hameed

Riffat

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The present study compared the performance and capability of thermophilic–mesophilic, temperature‐phased anaerobic digestion (TPAD) of wastewater sludge at 45 and 55 °C in terms of rate of hydrolysis and methanogenesis at two point interval times – December and January – with operational conditions kept constant (total solid content 6.5%, solids retention time 12.5 days). RESULTS The reduction of volatile solids (VS) was 77% at 45 °C TPAD‐1 and 72% at 55 °C TPAD‐II. The accumulation of ammonia and volatile fatty acids (VFAs; propionic acid) were observed to be below the inhibitory range (>3000 mg L−1), whereas the methane (CH4) production (45 °C, 3.55 ± 0.47 L CH4 L−1 day−1; 35 °C, 1.44 ± 0.12 L CH4 L−1 day−1) remained considerably higher in TPAD‐I than TPAD‐II. Furthermore, the TPAD‐II system suffered from certain degree of instability due to high level of total VFAs (6087 ± 1578 mg L−1), low buffering capacity, increased level of total NH3 (2982 ± 219 mg L−1) and free NH3 (246 ± 25 mg L−1), and relatively reduced CH4 production (1.69 ± 0.1 L L−1 day). The bacterial and archaeal population of the TPADs investigated by 454 pyrosequencing and Illumina sequencing showed, in both TPAD systems, a bacterial community dominated by Firmicutes, followed by Bacteriodetes, Proteobacteria, Synergistetes and Actiniobacteria. The archaeal community was dominated by Methanimicrobia (74–84% Methanosarcina) and Methanobacteria (15–27% Methanobacterium). A progression from genus Clostridium to Coprothermobacter and Tepidanaerobacter, and Methanocarcina to Methanothermobacter and Methanobacterium was observed in TPAD‐II. CONCLUSIONS This study proved the processes driving the dynamics of key microbial population and its correlation with hydrolytic functionality of TPAD systems. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 49%

Section: Bacterial Community Composition and Diversitymentioning

confidence: 96%

Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge

Hameed

Riffat

et al. 2019

J of Chemical Tech & Biotech

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“…Regarding the total number of species/OTUs, the calculation of the Shannon Index showed that the biodiversity (H′) of the archaeal community was lower compared with bacterial biodiversity in biogas reactors ( Table S2 ). This result is in line with other studies (Fernandez et al ., 1999 ; Bengelsdorf et al ., 2013 ; Gagliano et al ., 2015 ). Differences in feedstock mixtures did not affect the biodiversity notably as indicated by similar values for H′.…”

Section: Discussionmentioning

confidence: 99%

Functionally redundant but dissimilar microbial communities within biogas reactors treating maize silage in co‐fermentation with sugar beet silage

Langer

Ahmed

Einfalt

et al. 2015

Microbial Biotechnology

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Numerous observations indicate a high flexibility of microbial communities in different biogas reactors during anaerobic digestion. Here, we describe the functional redundancy and structural changes of involved microbial communities in four lab-scale continuously stirred tank reactors (CSTRs, 39°C, 12 L volume) supplied with different mixtures of maize silage (MS) and sugar beet silage (SBS) over 80 days. Continuously stirred tank reactors were fed with mixtures of MS and SBS in volatile solid ratios of 1:0 (Continuous Fermenter (CF) 1), 6:1 (CF2), 3:1 (CF3), 1:3 (CF4) with equal organic loading rates (OLR 1.25 kgVS m−3 d−1) and showed similar biogas production rates in all reactors. The compositions of bacterial and archaeal communities were analysed by 454 amplicon sequencing approach based on 16S rRNA genes. Both bacterial and archaeal communities shifted with increasing amounts of SBS. Especially pronounced were changes in the archaeal composition towards Methanosarcina with increasing proportion of SBS, while Methanosaeta declined simultaneously. Compositional shifts within the microbial communities did not influence the respective biogas production rates indicating that these communities adapted to environmental conditions induced by different feedstock mixtures. The diverse microbial communities optimized their metabolism in a way that ensured efficient biogas production.

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“…The literature covers the influence of thermophilic aerobic pre-degradation Pervin et al 2013), ultrasound pre-treatment (Braguglia et al 2012;Gagliano et al 2015a), ozonation (Kobayashi et al 2009) and thermal, alkaline or combined pre-treatment (Gagliano et al 2015b;Sun et al 2014). How to influence the digester for improved performance is a question of great value, and increased knowledge of the biogas-producing microbiota and the linkage with alteration in substrate characteristics withholds promising potential for further bioengineering innovations, allowing for process development and optimisation.…”

Section: Introductionmentioning

confidence: 99%

Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

Westerholm

Crauwels

Geel

et al. 2016

Appl Microbiol Biotechnol

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Comparative analyses of bacterial and archaeal community structures and dynamics in three biogas digesters during start-up and subsequent operation using microwaved, ultrasonicated or untreated waste activated sludge were performed based on 454 pyrosequencing datasets of part of 16S ribosomal RNA sequences and quantitative PCR. The pre-treatment increased the solubility, and thus the availability of the substrate for microbial degradation and significantly affected the succession of the anaerobic community structure over the course of the digestion. Bacteroidetes, Proteobacteria and Firmicutes were the dominant phyla in all digesters throughout operation. Proteobacteria decreased in relative abundance from 23-26 % to 11-13 % in association with enhanced substrate availability. Negative correlations between relative abundance of Alpha-, Beta- and Gammaproteobacteria and the substrate availability and/or biogas production were disclosed in statistical analyses. Clostridiales was the dominant order in Firmicutes, and Clostridiales, Clostridia and Firmicutes relative abundance and richness were shown to positively correlate with substrate availability and biogas generation. Methanogenic communities had a fairly restricted structure, highly dominated by Methanosaeta and Methanobrevibacter phylotypes. A gradual decline in Methanobrevibacter and increased representation of Methanosaeta concilii over time were particularly apparent in the digester receiving untreated waste activated sludge, whereas more diversified archaeal communities were maintained in the pre-treatment digesters. The quantitative PCR analyses revealed a methanogenic community distribution that coincided with the 454 pyrosequencing data.

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Microbial diversity in innovative mesophilic/thermophilic temperature-phased anaerobic digestion of sludge

Cited by 37 publications

References 44 publications

Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge

Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge

Functionally redundant but dissimilar microbial communities within biogas reactors treating maize silage in co‐fermentation with sugar beet silage

Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

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