Microbial diversity and related secondary metabolite gene assortment at an estuarine mangrove ecosystem

Sengupta, Sohan; Pramanik, Arnab; Nag, Sudip; Roy, Debojyoti; Bhattacharyya, Anish; Basak, Pijush; Bhattacharyya, Maitree

doi:10.1016/j.rsma.2020.101051

Cited by 4 publications

(4 citation statements)

References 41 publications

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“…The purity of the colonies was confirmed using a stereomicroscope (S8AP0; Leica). Bacterial cultures were maintained in marine broth (MB; peptone, 5 g; yeast extract, 1 g; C 6 H 5 FeO 7 , 0.1 g; NaCl, 19…”

Section: Isolation and Habitatmentioning

confidence: 99%

“…It has been estimated that the bacteria in mangrove sediments constitute up to 80 % of the total living biomass of these ecosystems [13][14][15][16] and play a key role in their functioning [12]. However, relatively few studies have focused on the exploration and characterization of the microbial diversity of mangrove sediments [7,10,11,13,14,[17][18][19][20][21][22][23][24][25][26][27] and the cultivable fraction of microbes therein [28][29][30][31][32][33][34]. The cultivable bacterial strains obtained from mangrove sediments include members of well-characterized genera, such as Bacillus, Halobacillus, Microbacterium, Novosphingobium, Paracoccus, Streptomyces, Thalassotalea and Vibrio [30,33,[35][36][37][38][39], and several novel genera, including Acidimangrovimonas, Mangroviflexus, Mangrovibacterium, Marisediminitalea, Mangrovicoccus, Mangrovitalea, Mangrovimonas and Zhengella [32,34,[40][41][42][43][44][45]; these data clearly conf...…”

Section: Introductionmentioning

confidence: 99%

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Kaustia mangrovi gen. nov., sp. nov. isolated from Red Sea mangrove sediments belongs to the recently proposed Parvibaculaceae family within the order Rhizobiales

Sefrji

Marasco

Michoud

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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We isolated a novel strain, R1DC25T, described as Kaustia mangrovi gen. nov. sp. nov. from the sediments of a mangrove forest on the coast of the Red Sea in Saudi Arabia. This isolate is a moderately halophilic, aerobic/facultatively anaerobic Gram-stain-negative bacterium showing optimum growth at between 30 and 40 °C, at a pH of 8.5 and with 3–5 % NaCl. The genome of R1DC25T comprises a circular chromosome that is 4 630 536 bp in length, with a DNA G+C content of 67.3 mol%. Phylogenetic analyses based on the 16S rRNA gene sequence and whole-genome multilocus sequence analysis of 120 concatenated single-copy genes revealed that R1DC25T represents a distinct lineage within the family Parvibaculaceae in the order Rhizobiales within the class Alphaproteobacteria . R1DC25T showing 95.8, 95.3 and 94.5 % 16S rRNA gene sequence identity with Rhodoligotrophos appendicifer , Rhodoligotrophos jinshengii and Rhodoligotrophos defluvii , respectively. The predominant quinone was Q-10, and the polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, as well as several distinct aminolipids and lipids. The predominant cellular fatty acids were C19 : 0 cyclo ω8c, a combination of C18 : 1 ω7c and/or C18 : 1 ω6c and C16 : 0. On the basis of the differences in the phenotypic, physiological and biochemical characteristics from its known relatives and the results of our phylogenetic analyses, R1DC25T (=KCTC 72348T;=JCM 33619T;=NCCB 100699T) is proposed to represent a novel species in a novel genus, and we propose the name Kaustia mangrovi gen. nov., sp. nov. (Kaustia, subjective name derived from the abbreviation KAUST for King Abdullah University of Science and Technology; mangrovi, of a mangrove).

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Section: Isolation and Habitatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kaustia mangrovi gen. nov., sp. nov. isolated from Red Sea mangrove sediments belongs to the recently proposed Parvibaculaceae family within the order Rhizobiales

Sefrji

Marasco

Michoud

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…Selecting the right primer set is crucial for accurately studying microbial communities in mangrove ecosystems using high-throughput sequencing of amplified 16S rRNA genes [78] . Using 16S rRNA sequencing [92] , we aimed to visualize bacterial communities in Sundarbans mangrove marine water for the first time. Molecular techniques [ 52 , 63 ] have the potential to be faster and more sensitive than traditional methods for identifying fish pathogens [1] .…”

Section: Introductionmentioning

confidence: 99%

Diversity of bacterial communities in the Sundarbans mangrove, Bangladesh, with special focus on pathogens affecting aquatic organisms

Hossain,

Mojumdar,

Farjana

et al. 2024

Comparative Immunology Reports

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“…Moreover, high index species diversity and limiting nutrient resources compel the indigenous microbial populations to establish an interspecies competition among them. This competition and strong selection pressure have resulted in the evolution of diverse survival strategies within the microbial communities (Sengupta et al, 2015, Sengupta et al, 2020.…”

Section: Introductionmentioning

confidence: 99%

Nanotube mediated cell-to-cell communication and cannibalism in Halobacillus sp. GSS1 isolated from Sundarbans, India: A cryptic story of survival under nutrient-limiting condition

Guria

Sengupta

Bhattacharyya

et al. 2020

Preprint

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Microorganisms play a self-protective role by evolving their genetic and metabolic machinery to thrive in extreme environmental habitats. Halophiles are such salt-loving extremophilic microorganisms able to adapt, survive, and tend to grow at high salt concentrations. In this study, we have isolated Halobacillus sp. GSS1 from Sundarbans mangrove, India having a strong salt-tolerant ability (up to 4M) in Zobell Marine 2216 medium. The salt adaptation mechanism of Halobacillus sp. was investigated by Confocal microscopy using [Na+] specific dye, Sodium Green indicating the salt-in strategy for their osmoadaptation. Electron microscopic studies revealed that a contact-dependent cell-to-cell communication was profound among the Halobacillus sp. under nutrient limiting condition. This communication is mediated by nanotube, which is highly recommended for the exchange of molecular information between the two individual bacteria. The existence of the ymdB gene strongly supports our claim for nanotube formation by Halobacillus sp. GSS1. Surprisingly, Halobacillus sp. not only utilizing the nanotubes for communication, rather they desperately use nanotubes as a survival weapon under nutrient limiting conditions by triggering cannibalism. This is the first-ever report on the existence of nanotube mediated cell-to-cell communication and cannibalism in any halophilic bacteria, isolated from Sundarbans mangrove forest, India.

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Microbial diversity and related secondary metabolite gene assortment at an estuarine mangrove ecosystem

Cited by 4 publications

References 41 publications

Kaustia mangrovi gen. nov., sp. nov. isolated from Red Sea mangrove sediments belongs to the recently proposed Parvibaculaceae family within the order Rhizobiales

Kaustia mangrovi gen. nov., sp. nov. isolated from Red Sea mangrove sediments belongs to the recently proposed Parvibaculaceae family within the order Rhizobiales

Diversity of bacterial communities in the Sundarbans mangrove, Bangladesh, with special focus on pathogens affecting aquatic organisms

Nanotube mediated cell-to-cell communication and cannibalism in Halobacillus sp. GSS1 isolated from Sundarbans, India: A cryptic story of survival under nutrient-limiting condition

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