Microbial degradation of two highly persistent fluorinated fungicides - epoxiconazole and fludioxonil

Alexandrino, Diogo A. M.; Mucha, Ana P.; Almeida, C. Marisa R.; Carvalho, Maria de Fátima

doi:10.1016/j.jhazmat.2020.122545

Cited by 36 publications

(18 citation statements)

References 64 publications

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“…Bacterial strains previously isolated from microbial consortia derived from an agricultural soil and enriched with EPO or FLU were used in this work ( Table 1 ) [ 7 ]. Isolated strains were revived from cryogenic stasis by spreading them in the culture medium in which they were isolated, either Plate-Count agar or Minimal Salts medium agar supplemented with the respective target pesticide.…”

Section: Methodsmentioning

confidence: 99%

“…Epoxiconazole (EPO) and fludioxonil (FLU) ( Figure S1 ) are two examples of fluorinated pesticides not readily degraded in the natural environment, as shown by their typically high half-lives in soils (over 353 and 218 days for EPO and FLU, respectively) [ 6 ] and by the scant literature reporting their productive biodegradation [ 7 , 8 ]. As a result, both these compounds have been reported to persist and accumulate in aquatic and terrestrial ecosystems [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…As a result, both these compounds have been reported to persist and accumulate in aquatic and terrestrial ecosystems [ 9 , 10 , 11 , 12 ]. We recently reported the successful enrichment of two bacterial consortia obtained from an agricultural soil with high capacity for the complete removal and defluorination of EPO and FLU (one consortium for each pesticide) in a wide range of pesticide concentrations, after 21 to 28 days of incubation [ 7 ]. Characterization of the cultivable microbial community of these consortia yielded a phylogenetically narrow group of bacterial strains, all accommodated within the Proteobacteria phylum, whose contribution to the observed catabolic phenotypes remained unresolved.…”

Section: Introductionmentioning

confidence: 99%

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Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

et al. 2021

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Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains Hydrogenophaga eletricum 5AE and Methylobacillus sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which H. eletricum may be the defluorinating strain and Methylobacillus sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely H. eletricum and Methylobacillus sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

et al. 2021

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“…Once in the environment, the persistence of pharmaceutical products can be influenced by (i) environmental factors (pH, soil characteristics, temperature, light incidence), (ii) physicochemical properties of the molecule (solubility (expressed by the octanol-water partition coefficient, (K ow )), molecular structure, polarity, pK a or pK b (in the case of having acid or base character), photo-stability, chemical stability, volatility (expressed by the Henry law constant (K H )), (iii) presence of other pharmaceuticals in the same matrix and (iv) presence and activity of microorganisms with the ability to degrade pharmaceuticals, metabolizing them as carbon sources or as co-metabolites [1,20,[60][61][62][63]. The presence of other biodegradable organic carbon sources can improve the removal/degradation of pharmaceutical compounds by enhancing the growth of degrading microorganisms or co-metabolic processes [64][65][66].…”

Section: Pharmaceuticals In the Environmentmentioning

confidence: 99%

“…Bioremediation has been reported as an efficient method for the remediation of organic pollutants such as hydrocarbons [170][171][172], pesticides [66,173,174], polychlorinated biphenyls [175] and pharmaceuticals [176,177]. This technology is based on three strategies: biostimulation, bioaugmentation or a combination of both.…”

Section: Bioremediation Processes As a New Remediation Technologymentioning

confidence: 99%

Pharmaceutical Compounds in Aquatic Environments—Occurrence, Fate and Bioremediation Prospective

Fernandes

Almeida

Salgado

et al. 2021

Toxics

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Various contaminants of emerging concern (CECs) have been detected in different ecosystems, posing a threat to living organisms and the environment. Pharmaceuticals are among the many CECs that enter the environment through different pathways, with wastewater treatment plants being the main input of these pollutants. Several technologies for the removal of these pollutants have been developed through the years, but there is still a lack of sustainable technologies suitable for being applied in natural environments. In this regard, solutions based on natural biological processes are attractive for the recovery of contaminated environments. Bioremediation is one of these natural-based solutions and takes advantage of the capacity of microorganisms to degrade different organic pollutants. Degradation of pollutants by native microorganisms is already known to be an important detoxification mechanism that is involved in natural attenuation processes that occur in the environment. Thus, bioremediation technologies based on the selection of natural degrading bacteria seem to be a promising clean-up technology suitable for application in natural environments. In this review, an overview of the occurrence and fate of pharmaceuticals is carried out, in which bioremediation tools are explored for the removal of these pollutants from impacted environments.

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Biodegradation of Environmental Pollutants by Autochthonous Microorganisms – A Precious Service for the Restoration of Impacted Ecosystems

Fernandes

Alexandrino

Mucha

et al. 2022

Assessing the Microbiological Health of Ecosystems

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Microbial degradation of two highly persistent fluorinated fungicides - epoxiconazole and fludioxonil

Cited by 36 publications

References 64 publications

Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

Pharmaceutical Compounds in Aquatic Environments—Occurrence, Fate and Bioremediation Prospective

Biodegradation of Environmental Pollutants by Autochthonous Microorganisms – A Precious Service for the Restoration of Impacted Ecosystems

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