Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation

Mohan, S. Venkata; Sistla, Srinivas; Guru, R.Kumar; Prasad, K. Krishna; Kumar, Chitta Suresh; Ramakrishna, S. V.; Sarma, P.N.

doi:10.1016/s0956-053x(02)00150-2

Cited by 44 publications

(23 citation statements)

References 10 publications

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“…Subsequently, some bacterial strains that degrade pyridine under aerobic or anaerobic conditions have been investigated, e.g., Nocardia [8], Micrococcus luteus [26], Rhodococcus opacus [5] and Pseudomonas [13,16]. Some previous research focused on the microbial transformation mechanism of pyridine.…”

Section: Introductionmentioning

confidence: 99%

Aerobic degradation of pyridine by a new bacterial strain, Shinella zoogloeoides BC026

Bai

Sun

Zhao

et al. 2009

J Ind Microbiol Biotechnol

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A new bacterial strain, Shinella zoogloeoides BC026, which utilizes pyridine as its sole carbon, nitrogen and energy source, was isolated from the activated sludge of a coking wastewater treatment plant. The BC026 strain completely degraded up to 1,806 mg/l of pyridine in 45.5 h. The optimum degradation conditions were pH 8.0 and temperature 30-35 degrees C. According to product monitoring and genetic analysis, the pyridine ring was cleaved between C(2) and N, resulting in 58% of pyridine-N being directly converted into ammonium. Providing glucose as the extra carbon source, the degradation of pyridine was not affected, while the growth of the strain was promoted, and 41% of pyridine-N was converted into ammonium with a C/N ratio of 35. The ammonium was utilized rapidly by the strain, and a portion of it was transformed into nitrate, then to nitrite, and finally to dinitrogen if enough extra carbon was provided. Considering these characteristics, this strain may accomplish heterotrophic nitrification and aerobic denitrification simultaneously.

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Section: Introductionmentioning

confidence: 99%

Aerobic degradation of pyridine by a new bacterial strain, Shinella zoogloeoides BC026

Bai

Sun

Zhao

et al. 2009

J Ind Microbiol Biotechnol

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show abstract

“…Therefore, the decomposition of pyridine has always been of interests in practice and in scientific research. There are huge references [3][4][5][6][7][8][9][10][11] on its decomposition by pyrolysis, biodegradation, and so on. Currently, heterogeneous photo-catalysis has often been in considerations on the destruction of pyridine in water based on the adsorption but the destructive techniques [12].…”

Section: Introductionmentioning

confidence: 99%

Kinetic study on the photo-catalytic degradation of pyridine in TiO2 suspension systems

Zhao

Suo-hong²,

Zhong³

et al. 2004

Catalysis Today

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“…The combined extract, were then centrifuged at 10,000 rpm for 5 min at 4 0 C and quantitatively transferred to a volumetric flask. The final volume was recorded and the hexane extract stored at 4 0 C. The total extract was evaporated to dryness under a nitrogen stream, the residue was dissolved in 1 mL of acetonitrile and injected into the HPLC [29]. Formaldehyde content in worms, sediment and water was analyzed by spectrophotometrically by formation of diacetyldihydrolutidine (DDL) [32].…”

Section: Extraction and Quantification Of Pyridine And Formaldehydementioning

confidence: 99%

Effect of Pyridine and Formaldehyde on a Macrophyte ( Lemna Minor L.) and a Sludge Worm ( Tubifex Tubifex Müller) in Fresh Water Microcosms

Singh¹

2008

Appl Ecol Env Res

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Singh et al.: Effect of pyridine and formaldehyd on Lemna minor L and Tubifex tubifex Müller in freshwater microcosmsAbstract. Pyridine, a suspected genotoxic and formaldehyde, a potent carcinogen, are present in several industrial wastewater including commercial solvent, resin and pesticide manufacturing industries. The extent of pyridine and formaldehyde toxicity in aquatic organisms is unknown. Therefore, in present study the toxicity of pyridine and formaldehyde were investigated in artificial aquatic ecosystem (microcosm) using two freshwater organisms of different trophic levels i.e. a common duckweed; Lemna minor L. and an oligochaete; Tubifex tubifex Müller. In microcosm, formaldehyde was found to be more toxic to chlorophyll (21 days IC50 = 0.08±0.02 mgL-1), protein (21 days IC50 = 0.15±0.02 mgL-1) and biomass (21 days IC50 = 0.18±0.02 mgL-1) contents of L. minor than pyridine. In addition, pyridine was found growth supportive to L. minor at concentration of 0.1-0.3 mgL-1. Formaldehyde was found more toxic than pyridine for T. tubifex. Lethal concentrations for formaldehyde were 0.08±0.004 (LC10-21 days), 0.2±0.03 (LC25-21 days) and 0.39±0.05 mgL-1 (LC50-21 days). For pyridine, lethal concentrations were 0.85±0.08 (LC10-21 days), 2.14±0.2 (LC25-21days) and 4.27±0.3 mgL-1 (LC50-21days). The concentration and time dependent decrease in growth rate, soluble protein and glycogen content was observed. To overcome the stress situation, high-energy requirement of worms lead to protein and glycogen catabolism. The results of present study might be an important consideration while assessing the hazards of materials to aquatic organisms or when deriving water quality criteria for aquatic organisms.

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Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation

Cited by 44 publications

References 10 publications

Aerobic degradation of pyridine by a new bacterial strain, Shinella zoogloeoides BC026

Aerobic degradation of pyridine by a new bacterial strain, Shinella zoogloeoides BC026

Kinetic study on the photo-catalytic degradation of pyridine in TiO2 suspension systems

Effect of Pyridine and Formaldehyde on a Macrophyte ( Lemna Minor L.) and a Sludge Worm ( Tubifex Tubifex Müller) in Fresh Water Microcosms

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