Microbial degradation of low-density polyethylene (LDPE) by Aspergillus clavatus strain JASK1 isolated from landfill soil

Gajendiran, Anudurga; Krishnamoorthy, Sharmila; Abraham, Jayanthi

doi:10.1007/s13205-016-0394-x

Cited by 204 publications

(120 citation statements)

References 20 publications

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“…The sequence being novel was submitted to GenBank. For phylogenetic analysis, the fungal isolates’ ITS gene sequences from this work and other sequences retrieved from the database were aligned using the software CLUSTAL W 1.830. The phylogenetical analysis was made using the neighbor-joining method.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Ojha

Pradhan

Singh

et al. 2017

Sci Rep

244

107

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Plastic in any form is a nuisance to the well-being of the environment. The ‘pestilence’ caused by it is mainly due to its non-degradable nature. With the industrial boom and the population explosion, the usage of plastic products has increased. A steady increase has been observed in the use of plastic products, and this has accelerated the pollution. Several attempts have been made to curb the problem at large by resorting to both chemical and biological methods. Chemical methods have only resulted in furthering the pollution by releasing toxic gases into the atmosphere; whereas; biological methods have been found to be eco-friendly however they are not cost effective. This paves the way for the current study where fungal isolates have been used to degrade polyethylene sheets (HDPE, LDPE). Two potential fungal strains, namely, Penicillium oxalicum NS4 (KU559906) and Penicillium chrysogenum NS10 (KU559907) had been isolated and identified to have plastic degrading abilities. Further, the growth medium for the strains was optimized with the help of RSM. The plastic sheets were subjected to treatment with microbial culture for 90 days. The extent of degradation was analyzed by, FE-SEM, AFM and FTIR. Morphological changes in the plastic sheet were determined.

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Section: Methodsmentioning

confidence: 99%

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Ojha

Pradhan

Singh

et al. 2017

Sci Rep

244

107

View full text Add to dashboard Cite

show abstract

“…The rate and efficiency of polymer degradation were determined by pH alteration in media, CO 2 evolution, weight loss, SEM and FT-IR analysis. Similarly, Gajendiran et al (2016) isolated a fungus from landfill soil, identified as A. clavatus (strain JASK1). They exploited A. clavatus for LDPE degradation by incubating the samples for 90 days and evaluated the biodegradation by means of CO 2 evolution, SEM and AFM analysis.…”

Section: Polyethylenementioning

confidence: 99%

Review on the current status of polymer degradation: a microbial approach

Pathak

Bithel

2017

Bioresour. Bioprocess.

564

241

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Inertness and the indiscriminate use of synthetic polymers leading to increased land and water pollution are of great concern. Plastic is the most useful synthetic polymer, employed in wide range of applications viz. the packaging industries, agriculture, household practices, etc. Unpredicted use of synthetic polymers is leading towards the accumulation of increased solid waste in the natural environment. This affects the natural system and creates various environmental hazards. Plastics are seen as an environmental threat because they are difficult to degrade. This review describes the occurrence and distribution of microbes that are involved in the degradation of both natural and synthetic polymers. Much interest is generated by the degradation of existing plastics using microorganisms. It seems that biological agents and their metabolic enzymes can be exploited as a potent tool for polymer degradation. Bacterial and fungal species are the most abundant biological agents found in nature and have distinct degradation abilities for natural and synthetic polymers.

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“…Biodegradation of Polythene bag and plastic cup by Rhizopus sps have been reported (Kannahi and Sudha 2013). In this study, we have chosen fungus for bioremediation because they are very versatile and play a crucial role in the decay of lignocelluloses polymer, and have extensive metabolic capabilities (Gajendiran et al 2016). Degradation of low density polyethylene by Rhizopus oryzae NS5 has not done yet.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of thermally treated low density polyethylene by fungus Rhizopus oryzae NS 5

et al. 2017

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Polythene is considered as one of the important object used in daily life. Being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. In the present study, biodegradation of low-density polyethylene (LDPE) have been performed using fungal lab isolate Rhizopus oryzae NS5. Lab isolate fungal strain capable of adhering to LDPE surface was used for the biodegradation of LDPE. This strain was identified as Rhizopus oryzae NS5 (Accession No. KT160362). Fungal growth was observed on the surface of the polyethylene when cultured in potato dextrose broth at 30°C and 120 rpm, for 1 month. LDPE film was characterized before and after incubation by Fourier transform infrared spectroscopy, scanning electron microscopy, atomic force microscopy and universal tensile machine. About 8.4 ± 3% decrease (gravimetrically) in weight and 60% reduction in tensile strength of polyethylene was observed. Scanning electron microscope analysis showed hyphal penetration and degradation on the surface of polyethylene. Atomic force microscope analysis showed increased surface roughness after treatment with fungal isolate. A thick network of fungal hyphae forming a biofilm was also observed on the surface of the polyethylene pieces. Present study shows the potential of Rhizopus oryzae NS5 in polyethylene degradation in eco friendly and sustainable manner.

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Microbial degradation of low-density polyethylene (LDPE) by Aspergillus clavatus strain JASK1 isolated from landfill soil

Cited by 204 publications

References 20 publications

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Review on the current status of polymer degradation: a microbial approach

Biodegradation of thermally treated low density polyethylene by fungus Rhizopus oryzae NS 5

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