Microbial Degradation of Acetamiprid by Ochrobactrum sp. D-12 Isolated from Contaminated Soil

Abstract

Neonicotinoid insecticides are one of the most important commercial insecticides used worldwide. The potential toxicity of the residues present in environment to humans has received considerable attention. In this study, a novel Ochrobactrum sp. strain D-12 capable of using acetamiprid as the sole carbon source as well as energy, nitrogen source for growth was isolated and identified from polluted agricultural soil. Strain D-12 was able to completely degrade acetamiprid with initial concentrations of 0–3000 mg·L−1 within 48 h. Haldane inhibition model was used to fit the special degradation rate at different initial concentrations, and the parameters q max, K s and K i were determined to be 0.6394 (6 h)−1, 50.96 mg·L−1 and 1879 mg·L−1, respectively. The strain was found highly effective in degrading acetamiprid over a wide range of temperatures (25–35°C) and pH (6–8). The effects of co-substrates on the degradation efficiency of acetamiprid were investigated. The results indicated that exogenously supplied glucose and ammonium chloride could slightly enhance the biodegradation efficiency, but even more addition of glucose or ammonium chloride delayed the biodegradation. In addition, one metabolic intermediate identified as N-methyl-(6-chloro-3-pyridyl)methylamine formed during the degradation of acetamiprid mediated by strain D-12 was captured by LC-MS, allowing a degradation pathway for acetamiprid to be proposed. This study suggests the bacterium could be a promising candidate for remediation of environments affected by acetamiprid.

Co‐transport of Pesticide Acetamiprid and Silica Nanoparticles in Biochar‐Amended Sand Porous Media

“…The microorganisms in the biochar likely induced biodegradation of acetamiprid. The simulated degradation rate, μ w was greater at pH 6.4 than at pH 4 and 10 in the biochar‐amended sand (Table 1), in agreement with the experimental results in Wang et al (2013a).…”

“…Hydrolysis and photolysis of acetamiprid should also be minor in the biochar‐amended sand because the experimental conditions are similar. A number of studies (Liu et al, 2011; Wang et al, 2013a, 2013b; Yang et al, 2013; Zhou et al, 2014b) showed that the primary degradation pathway for the acetamiprid is aerobic soil metabolism. The microorganisms in the biochar likely induced biodegradation of acetamiprid.…”

Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid

“…Microbiological degradation plays key roles in the dissipation of acetamiprid from the environment, as well as in the detoxification of acetamiprid. Many prokaryotic and eukaryotic microorganisms have been reported to be capable of degrading acetamiprid (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). Strain D-2, previously isolated in our laboratory, could degrade over 99% of 0.22 mM acetamiprid in 3 days, showing that it possesses a very high degradation capability.…”

“…The environmental fate of acetamiprid has attracted considerable attention, and microbial degradation is the key means for the dissipation of acetamiprid from the environment (15,16). In recent years, several microbial strains capable of degrading acetamiprid have been isolated, and the catabolic pathways of acetamiprid have been extensively studied (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). Four catabolic pathways of acetamiprid are illustrated in Fig.…”

Optimization of photochemical decomposition acetamiprid pesticide from aqueous solutions and effluent toxicity assessment by Pseudomonas aeruginosa BCRC using response surface methodology

“…John et al ( 2016 ) in Kerala by coagulation and flocculation process (John et al 2016 ). Wang ( 2013 ) in China used biological processes for removing acetamiprid (Wang et al 2013 ). Similarly, Shanping ( 2014 ) and Fasnabi ( 2012 ) employed low-temperature heat plasma and ozonation process, respectively for removal of acetamiprid (Fasnabi et al 2012 ; Shanping et al 2014 ).…”