Microbial Conversion with Cofactor Regeneration using Genetically Engineered Bacteria

Abstract

In the synthesis of fine chemicals, biotransformations have been recognized as useful methods and applied for large‐scale manufacturing. Especially, the recent development of recombinant DNA technology has greatly expanded the whole microbial cell processes for manufacturing fine chemicals. The whole‐cell approaches have been applied not only to single conversion process but also to biotransformations requiring cofactor regeneration, such as reductions with NADH or NADPH and phosphorylations with ATP. The whole microbial cell conversion process with cofactor regeneration enables the production of complicated compounds, such as sugar nucleotides and oligosaccharides, on an industrial scale. 1 Introduction 2 NAD(P)H Regeneration 3 ATP Regeneration 4 NTP Regeneration System 5 Conclusion

Oxalyl‐CoA Decarboxylase Enables Nucleophilic One‐Carbon Extension of Aldehydes to Chiral α‐Hydroxy Acids

“…To this end, employing whole-cell catalysts may prove to be advantageous, providing not only the cascade enzymes but also ATP regeneration and a CoA pool without the addition of purified enzymes and cofactors. [22] Altogether, our study expands the spectrum of ThDPdependent transformations by nucleophilic C 1 -extensions, which gives access to a-hydroxy acids that are valuable chiral building blocks and showcases ways to establish in vitro and in vivo platforms for the continuous production of these compounds in the future.…”

Protein Engineering Applications on Industrially Important Enzymes: Candida methylica FDH as a Case Study

“…Formate dehydrogenase (FDH) (Bolivar et al, 2007;Gül-Karagüler et al, 2001;Seelbach et al, 1996;), phosphite dehydrogenase (PTDH) (Johannes et al, 2007;Relyea et al, 2005;Woodyer et al, 2006), alcohol dehydrogenase (ADH), glucose-6 phosphate dehydrogenase, glucose dehydrogenase(GDH) (Endo &Koizumi, 2001;Xu et al, 2007) are currently available systems.…”

Improved expression of recombinant cytochrome P450 monooxygenase in Escherichia coli for asymmetric oxidation of sulfides

“…Whole cell was often used as biocatalyst, because the whole-cell approaches have been applied not only to single enzyme conversion process but also to biotransformations requiring cofactor regeneration, such as reductions with NADH or NADPH [19].…”