Microbial contributions to oxalate metabolism in health and disease

Liu, Menghan; Devlin, Joseph C.; Hu, Jiyuan; Volkova, Angelina; Battaglia, Thomas; Byrd, Allyson L.; Loke, P’ng; Li, Huilin; Ruggles, Kelly V.; Tsirigos, Aristotelis; Nazzal, Lama

doi:10.1101/2020.01.27.20018770

Cited by 3 publications

(3 citation statements)

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“…However, the genes encoding FCR and OXC were substantially more prevalent and more abundant than the genes encoding oxalate oxidase and decarboxylase in the gut. Among the 660 subjects, OXC can be detected in the metagenome of 554 subjects (84%) and FCR can be detected in 581 subjects (88%) (Liu et al, 2020). These data suggested that OMBS with OXC and FCR are the predominant bacteria in the healthy human gut.…”

Section: Discussionmentioning

confidence: 84%

“…Oxalate decarboxylases and oxalate oxidases are members of the cupin superfamily of proteins and the two enzymes show high similarity at the amino acid level (Burrell et al, 2007). The genes encoding the four enzymes were widely present in the healthy gut microbiome on the basis of the analysis of 660 subjects (Liu et al, 2020). However, the genes encoding FCR and OXC were substantially more prevalent and more abundant than the genes encoding oxalate oxidase and decarboxylase in the gut.…”

Section: Discussionmentioning

confidence: 99%

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Abundance, Functional, and Evolutionary Analysis of Oxalyl-Coenzyme A Decarboxylase in Human Microbiota

et al. 2020

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Oxalic acid and its oxalate salts have been linked to kidney stones and other health problems and about 80% kidney stones are made up of calcium oxalate. Oxalyl coenzyme A decarboxylase (OXC) is a key enzyme in the catabolism of oxalate. In this study, we performed bioinformatic and biochemical analysis of OXC. First, we mined the OXC sequences from a public protein database and collected 1396 putative OXC sequences. These sequences were widely spread and mainly distributed in Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, and Betaproteobacteria and classified into seven clusters. The phylogenetic relationship and evolutionary rate of the 7 clusters showed that OXC are highly conserved. Second, the abundance of the different clusters of OXC was explored in 380 human microbiome datasets, which showed that OXCs in Cluster 1 were relatively high in the gut while OXCs in Clusters 2-4 were relatively enriched in the vagina. Third, we measured the activity of one OXC from Mycobacterium mageritense (OXCmm) in Cluster 3, in which there was no experimentally characterized enzymes. Mutation analysis showed that OXCmm shared the same active sites with the OXC from Oxalobacter formigenes. Taken together, this analysis provides a better insight into the distribution and catalysis of OXC and further potential alternative application of OXC active bacteria as probiotics in the management of kidney stone disease.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Abundance, Functional, and Evolutionary Analysis of Oxalyl-Coenzyme A Decarboxylase in Human Microbiota

et al. 2020

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“…According to the analysis of 660 subjects, the four genes encoding the enzymes were widely present in the healthy gut microbiome [39]. In the metagenomes of 660 subjects, Oxc can be detected in 554 (84%) and Frc in 581 (88%).…”

Section: Analysis Of Transcription and Function Of The Oxc And Frc Ge...mentioning

confidence: 99%

Probiotic Oxalate-Degrading Bacteria: New Insight of Environmental Variables and Expression of The Oxc and Frc Genes on Oxalate Degradation Activity

Karamad¹,

Mousavi-Khaneghah²,

Miller³

2022

Preprint

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Oxalate, a compound produced by many edible plants and as a terminal metabolite in the liver of mammals, is a toxin that has a detrimental role to human health. Humans and other mam-mals do produce the enzymatic machinery to degrade oxalate. However, numerous oxa-late-degrading bacteria reside in the mammalian gut and thus provide an important function for hosts. The current review focuses on the environmental factors that influence the efficacy of pro-biotic oxalate-degrading bacteria, relative to oxalate metabolism. We describe the mechanism of oxalate catabolism and its consumption by obligate and facultative anaerobic oxalate-degrading bacteria, in both in vitro and in vivo environments. We also explore the environmental variables that impact oxalate degradation. Studies on single species degrade oxalate have not shown a strong impact on oxalate metabolism especially in high oxalate conditions such as consumption of foods high in oxalate (such as coffee and chocolate for humans or halogeton in animal feed). Considering effective variables which enhance oxalate degradation could be used in application of effective probiotic as a therapeutic tool in individuals with hyperoxaluria. This study indicates probiotics can be considered a good source of naturally occurring oxalate degrading agent in human colon

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Enteric Hyperoxaluria and Kidney Stone Management in Inflammatory Bowel Disease

Lin

Liu

et al. 2020

Curr Treat Options Gastro

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Microbial contributions to oxalate metabolism in health and disease

Cited by 3 publications

References 102 publications

Abundance, Functional, and Evolutionary Analysis of Oxalyl-Coenzyme A Decarboxylase in Human Microbiota

Abundance, Functional, and Evolutionary Analysis of Oxalyl-Coenzyme A Decarboxylase in Human Microbiota

Probiotic Oxalate-Degrading Bacteria: New Insight of Environmental Variables and Expression of The Oxc and Frc Genes on Oxalate Degradation Activity

Enteric Hyperoxaluria and Kidney Stone Management in Inflammatory Bowel Disease

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