Microbial contamination of removable dental prosthesis at different interval of usage: An in vitro study

Abstract

Background:Loss of teeth affects the individual's health. Many factors determine the need to wear a removable dental prosthesis. Due to nature of design, age and lack of awareness, prosthesis often are neglected to maintain in an aseptic condition leading to microbial contamination. This provided an impetus for the present study with the aim of determining the microbial contamination of removable dental prosthesis.Methodology:Total, 45 patients wearing removable dental prosthesis were randomly selected. Patients were divided into three groups as per duration of usage since 1 month, 6 month and ≥1 year. Sterile cotton swab moistened with phosphate buffer saline (PBS) was used to collect swab from the fitting areas of prosthesis. Swab samples was inserted immediately into the sterile tube containing 1 ml of PBS solution, 10 μl PBS is inoculated on the blood agar and MacConkey agar plates using spread plate technique. Samples were cultured and incubated at 37°C for 48 h. Calibrated microbiologist isolated, identified and counted microorganisms using colony counter. Depending on the nature of data, statistical analysis was done applying Kruskal–Wallis test, Mann–Whitney U-test and Chi-square test.Result:Streptococcus species and Staphylococcus aureus were the common microorganisms isolated in all three groups and was statistically significant at P <0.05. Candida albicans, Diptheroid, Escherichia coli, Micrococcus species were isolated from Group II and Group III.Conclusion:There is a linear increase in microbial contamination of removable dental prosthesis as the duration of usage increases and might increase the susceptibility of individuals' to many diseases.

Silver Nanoparticles Addition in Poly(Methyl Methacrylate) Dental Matrix: Topographic and Antimycotic Studies

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Elemental ion release and cytotoxicity of antimicrobial acrylic resins incorporated with nanomaterial

“…The adhesion of microorganisms on the surfaces of dental prostheses favours the development of local diseases such as caries and stomatitis and systemic diseases such as bacterial endocarditis and aspiration pneumonia . The susceptibility to infection is increased for geriatric patients with a compromised immune system or limited manual dexterity, making good oral hygiene difficult …”

Relative presence of Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens in biofilm of complete dentures

“…[23] Similarly, bacterial species from Diphtheroid , E. coli , and Micrococcus genus were found by culture-based protocols in biofilms of partial dentures. [24] However, due to its inherent limitation, 16S rRNA gene sequencing analysis is gaining more attention in clinical research. We performed the 16S rRNA gene sequencing analysis only on a limited sample size as a way to identify commonly occurring pathogenic bacteria.…”