Microbial Contamination of Contact Lens Care Systems

Donzis, Paul B.; Mondino, Bartly J.; Weissman, Barry A.; Bruckner, David A.

doi:10.1016/0002-9394(87)90219-4

Cited by 159 publications

(62 citation statements)

References 23 publications

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“…qualitative shift towards Gram-negative organisms, which constitute the majority of organisms isolated from lens storage cases and various in-use lens care solutions. [14][15][16] The observation that lens wearers did not yield sig nificantly more Gram-negative bacteria in this study may well reflect the efficiency of host antimicrobial defences at the ocular surface rather than fastidious attention to hygiene by the subjects studied.…”

Section: Discussionmentioning

confidence: 88%

Quantitative alterations of the commensal eye bacteria in contact lens wear

Larkin

Leeming

1991

Eye

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SummaryA study was performed on the commensal external eye flora in 34 long-term contact lens wearers and a matched control group, in order to identify the effect of lens wear.Samples were taken from the lid margin and conjunctiva by semi-quantitative, and tear film by quantitative methods. Cultured bacteria were identified with particular attention to coagulase-negative staphylococci. No qualitative alteration in the com mensal bacteria was found, although lens wearers were found to have a significantly higher number of species at all sites than controls. Significant quantitative changes were identified on the lid margin, with particularly high counts in some lens wearers, and tear film. Increased numbers of bacteria obtained from the conjunctiva were not statistically significant. Quantitative changes in the tear film are thought to be secon dary to changes at the lid margin, for which no explanation is apparent.

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Section: Discussionmentioning

confidence: 88%

Quantitative alterations of the commensal eye bacteria in contact lens wear

Larkin

Leeming

1991

Eye

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“…aeruginosa is one of the most common contaminants recovered from contact lens cases and one of the most frequent etiological agents of corneal ulcers associated with contact lens wear [1,17,18]. While the pathogenesis of contact lens-related Pseudomonas keratitis remains unclear, bacterial contamination of the eye appears to play a major role [2,20].…”

Section: Discussionmentioning

confidence: 99%

“…During contact lens wear, organisms can gain access to the eye from the environment via contamination of the lens, lens case, and lens care solution. Studies of patients with contact lens-associated corneal ulcers have shown contamination of the care systems [17,18]. Microbial contamination of the care system may represent the source of the infecting organisms in corneal ulcers associated with contact lens wear [15].…”

Section: Discussionmentioning

confidence: 99%

“…Microbial contamination of the care system may represent the source of the infecting organisms in corneal ulcers associated with contact lens wear [15]. Contamination rates range from 20% to 80% in asymptomatic and symptomatic patients, with P. aeruginosa emerging as the predominant pathogen in the presence of other bacteria, fungi, and Acanthamoeba [1,17,18,20]. The presence of microorganisms in the contact lens cases does not necessarily always imply the occurrence of keratitis.…”

Section: Discussionmentioning

confidence: 99%

“…All the fungal strains were isolated from corneal specimens at the Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirapalli, India. Along with the fungal strains, we chose to test a corneal isolate of P. aeruginosa, one of the most common contaminants of contact lens care systems [17,18]. The soft contact lens disinfectants analyzed in the study are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

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Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions

Pinna

Usai

Zanetti

et al. 2013

J Infect Dev Ctries

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Introduction: We aimed to investigate the susceptibility of a combined inoculum of Pseudomonas aeruginosa and different fungal strains to 6 soft contact lens disinfectants. Methodology: One corneal isolate of P. aeruginosa and 13 corneal fungal isolates (9 Aspergillus spp, 3 Fusarium spp, 1 Curvularia sp.) were used. The following solutions were tested: Arion Cronos, Complete RevitaLens, Dua Elite, Opti-Free Express, Regard, Oxysept Comfort, and Oxysept Comfort without catalase. The effect of the solutions was assessed on a combined inoculum of P. aeruginosa plus 1 fungal strain. Suspensions of P. aeruginosa and fungi were made in the solutions (1x10 6 colony-forming units/mL). After 1 hour (Arion Cronos only), 6, 8, and 24 hours, aliquots of suspension were removed and seeded on Luria-Bertani and Sabouraud agar plates. Results: After 6 hours' exposure, all the solutions but Dua Elite and Oxysept Comfort eradicated P. aeruginosa. Conversely, apart from 3% hydrogen peroxide-based Oxysept Comfort without catalase, which eradicated all the fungi tested after 6 hours, all the other solutions were partly ineffective at killing some of the fungal isolates, even after 24 hours' exposure. Conclusions: Most contact lens disinfectants may be ineffective if contact lens care systems become co-contaminated with P. aeruginosa and fungi. In our experiment, only exposure to 3% hydrogen peroxide without neutralizer for at least 6 hours was always able to kill a combined inoculum of P. aeruginosa and different fungal strains.

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Association Between Cultures of Contact Lens and Corneal Scraping in Contact Lens–Related Microbial Keratitis

Das¹

2007

Arch Ophthalmol

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Microbial Contamination of Contact Lens Care Systems

Cited by 159 publications

References 23 publications

Quantitative alterations of the commensal eye bacteria in contact lens wear

Quantitative alterations of the commensal eye bacteria in contact lens wear

Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions

Association Between Cultures of Contact Lens and Corneal Scraping in Contact Lens–Related Microbial Keratitis

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