Microbial contamination of cell cultures: A 2 years study

Mirjalili, Ali; Parmoor, E.; Bidhendi, Soheila Moradi; Sarkari, Bahadoor

doi:10.1016/j.biologicals.2005.01.004

Cited by 42 publications

(34 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent report obtained a 19% contamination rate by Mycoplasma species, although, by incorporating the mixed contamination results in which Mycoplasma was the main isolate, led to the Mycoplasma contamination rate rising to 22% (Mirjalili et al, 2005). However, in the present study our results revealed an overall Mycoplasma contamination of 4%.…”

Section: Discussioncontrasting

confidence: 76%

“…There are several recent reports of microbial contamination in cell cultures (Langdon, 2004;Mirjalili et al, 2005) and the most frequently isolated microorganisms are Mycoplasma, other bacteria from the human skin, airborne bacterial spores, airborne fungal spores and most infrequently gram negative bacteria (Halls, 2004;Owers et al, 2004). In our study, we observed that the majority of microorganisms recovered from stem cell cultures were saprophytic pathogens, which are inhabitants of normal human skin, mucosal, oropharynx and also in the environment.…”

Section: Discussionmentioning

confidence: 62%

See 1 more Smart Citation

Microbiological contamination in stem cell cultures

Cobo

Cortés

Cabrera

et al. 2007

Cell Biology International

View full text Add to dashboard Cite

Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential form of contamination in these cell products is by bacteria (including Mycoplasma), yeast and fungi. In our study, 32 stem cell lines and feeder cell lines were analysed. There were 19 contaminated cell passages (12%). The main contaminants were gram positive cocci and Mycoplasma species, followed by gram negative rods and gram positive rods. The Mycoplasma contamination rate was 4%. Stem cell banks and other research centres aim to screen all processed stem cell lines for these microorganisms, and to assure that no contaminants are introduced in the banking procedures. It is a standard part of current good practice in stem cell banks to carry out routine microbiological controls of the stem cell lines and to work in a controlled environment to reduce the probability of contamination in the final product.

show abstract

Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 62%

Microbiological contamination in stem cell cultures

Cobo

Cortés

Cabrera

et al. 2007

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Of these, Mycoplasma is considered as one of the major threats for cell cultures [2,3]. A recent study reported a 19% contamination rate by Mycoplasma species and the rate increased to 22% if the mixed contamination is taken into account, in which Mycoplasma is the main isolate [4]. Thus, Mycoplasma contamination can interfere with a number of biological parameters and influence the final data during routine cultivation or experimental investigation [3].…”

Section: Introductionmentioning

confidence: 98%

Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Kim¹,

Jung²,

Song³

et al. 2009

Electrophoresis

View full text Add to dashboard Cite

We present real-time, rapid detection of Mycoplasma pneumonia in PBS inside a Y-channel PDMS microfluidic device via optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed with serially diluted M. pneumonia solutions using highly carboxylated polystyrene particles of 390 and 500 nm diameter conjugated with monoclonal anti-M. pneumonia. Proximity optical fibers were located around the viewing cell of the device, which were used to measure the increase in 45 degrees forward light scattering of the aggregated particles. The detection limit are slightly less than 50 pg mL(-1) both for 390 and 500 nm microspheres and the detection time do not exceed 90 s.

show abstract

“…The standardised procedures and training protocols should be followed strictly on a timely basis. 13,51 Improper handling usually leads to mixing and contamination, and therefore utmost care should to taken by avoiding conversations, coughing, sneezing, spilling of medium on surfaces and accidental touching of pipettes to surfaces. [52][53][54] …”

Section: Elimination and Prevention Of Microbial Contaminationmentioning

confidence: 99%

“…The contamination is reported to spread through airborne particles and non-sterile surfaces mainly. 13 In a study on fibroblasts and keratinocyte cell lines, 32.35% cultures were contaminated, and among these, the mycoplasma contaminated cultures were 17.65%, followed by fungal (8.82%) and bacterial (5.88%) contaminants.14 Aspergillus tops the list of fungal contamination of cell cultures. …”

mentioning

confidence: 99%

Microbial and Viral Contamination of Animal and Stem Cell Cultures: Common Contaminants, Detection and Elimination

Mahmood

Ali

2017

JSRT

View full text Add to dashboard Cite

The microbial contamination of animal cell culture, including stem cells, such as the blood stem cells, is a common problem of cell culture laboratories. It is important to identify the types of microorganisms commonly contaminating the cell culture and the sources of contaminating microorganisms, as well as see their effects on cells in culture. This mini review provides a short account of common microbial contaminations of stem cell and animal cell cultures, their detection and elimination, as well as standard practices to ensure a healthy and sterile cell culture, and a brief account of methods used for identification of contaminating microorganisms in cell culture. spoil a culture medium. Keywords7 Residues from germicides used to disinfect incubators, equipments and labs, and impurities in gases used in CO 2 incubators have also been found to affect cell culture.8 However, the major source of microbial contamination is documented to arise by contact with non-sterile supplies, media and solution, chemicals and equipments, and by the particulate matter and aerosols that fall out during transportation and incubation.9 According to a study, 1960-70, the new born bovine serum obtained from commercial suppliers was found to be a major source of bacterial, particularly the mycoplasma, contamination. 10One of the most common contaminants, and also difficult to detect, that has been reported in literature is Mycoplasma contamination. 11Mycoplasma is an intracellular bacterium that may go unnoticed for many passages. Its presence can change several cell properties such as the growth, metabolism, morphology, and genome structure. In literature, the incidences of Mycoplasma contamination have been reported over a period of time since 1956. 12 The incidence of Mycoplasma contamination was 19%, as compared with fungal (8%) and other bacterial (4%) contaminations. The contamination is reported to spread through airborne particles and non-sterile surfaces mainly. 13 In a study on fibroblasts and keratinocyte cell lines, 32.35% cultures were contaminated, and among these, the mycoplasma contaminated cultures were 17.65%, followed by fungal (8.82%) and bacterial (5.88%) contaminants.14 Aspergillus tops the list of fungal contamination of cell cultures. 15M. orale is reported to be the most common contamination that can spread from human oral cavity, and hence making the lab workers a major source of this contamination.10 Bacterial species (Bacillus sp., Enterococcus sp. and Staphylococcus sp) are similarly some major contaminants of animal cell cultures.9 A list of common microbial species and their major sources that have been reported to contaminate animal/stem cell culture is provided in Table 1 & 2, respectively.

show abstract

Microbial contamination of cell cultures: A 2 years study

Cited by 42 publications

References 4 publications

Microbiological contamination in stem cell cultures

Microbiological contamination in stem cell cultures

Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Microbial and Viral Contamination of Animal and Stem Cell Cultures: Common Contaminants, Detection and Elimination

Contact Info

Product

Resources

About