Microbial composition of the activated sludge of Moscow wastewater treatment plants

Abstract

The contribution of the major technologically important microbial groups (ammonium and nitrite oxidizing, phosphate accumulating, foam inducing, and anammox bacteria, as well as plancto mycetes and methanogenic archaea) was characterized for the aeration tanks of the Moscow wastewater treatment facilities. FISH investigation revealed that aerobic sludge were eubacterial communi ties; the metabolically active archaea contributed insignificantly. Stage II nitrifying microorganisms and planctomycetes were significant constituents of the bacterial component of activated sludges, with Nitro bacter spp. being the dominant nitrifiers. No metabolically active anammox bacteria were revealed in the sludge from aeration tanks. The sludge from the aeration tanks using different wastewater treatment technol ogies were found to have differing characteristics. Abundance of the nitrifying and phosphate accumulating bacteria in the sludge generally correlated with microbial activity in microcosms and with efficiency of nitro gen and phosphorus removal from wastewater. The highest microbial numbers and activity were found in the sludge of the tanks operating according to the technologies developed in the universities of Hannover and Cape Town. The activated sludge from the Novokur'yanovo facilities, where abundant growth of filamentous bacteria resulted in foam formation, exhibited the lowest activity. The group of foaming bacteria included Gordonia spp. and Acinetobacter spp utilizing petroleum and motor oils, Sphaerotilus spp. utilizing unsatur ated fatty acids, and Candidatus 'Microthrix parvicella'. Thus, the data on abundance and composition of metabolically active microorganisms obtained by FISH may be used for the technological control of waste water treatment.Keywords: wastewater treatment plants, activated sludge, nitrifying bacteria, phosphate accumulating organ isms, foaming bacteria, fluorescent in situ hybridization (FISH)

Less abundant bacterial groups are more affected than the most abundant groups in composted tannery sludge-treated soil

“…Our study also found other important and potential groups that are observed in polluted environments. Thus, Chloroflexi was found in sites with the application of activated sludge from the wastewater treatment industry 33 , and Anaerolinea was found in sites contaminated with petroleum 34 .…”

Technology of Wastewater Treatment Production of Vegetable Oils and Fats and Evaluation of Aeration Tank Efficiency on the Basis of Microanalysis of Activated Sludge

“…Violation of internal dynamic equilibrium in sludge ecosystem promotes the active sludge foaming process which increases the sludge index. In global experience, filamentary foaming of activated sludge (existence of filamentary microorganisms) is a widely known problem of the biological treatment of wastewater [Kallistova et al 2014; Nastro, Gambino 2017]. Filamentary microorganisms include bacteria and fungi.…”

Management of industrial wine residues: Physicochemical, bacterial and fungal dynamics during composting processes

“…Considering that sludge represents approximately 5% of the total residues produced by the winery (Bharathiraja et al, 2020), the composting of grape stalks, pomace and sludge rich in nitrogen has also been used to reduce operational costs (Bertran et al, 2004;Bustamante et al, 2007Bustamante et al, , 2009. Winery sludge is an alkaline and highly humid substrate with a high amount of nitrogen and an elevated number of microorganisms (Pascual et al, 2018), including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospira, Dechloromonas, Arcobacter, Nitrobacter, Planctomycetes, Chloro exi, and several microorganisms that participate in the nitrogen cycle (Gao et al, 2016;Kallistova et al, 2014;Neklyudov et al, 2008) . These phylogenetic groups have also been reported in the early stages of compost, suggesting that sludge could be a suitable supplement for the composting process of winery residues, improving the conditions of the process, increasing bene cial microorganisms, and reducing sludge management costs.…”