Microbial community structure in a full-scale anaerobic treatment plant during start-up and first year of operation revealed by high-throughput 16S rRNA gene amplicon sequencing

Fykse, Else-Marie; Aarskaug, Tone; Madslien, Elisabeth Henie; Dybwad, Marius

doi:10.1016/j.biortech.2016.09.118

Cited by 53 publications

(10 citation statements)

References 36 publications

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“…This could be explained by the residual DNA of the dead cells, low DNA concentration in cells and amplication biases. 9,16,36 The alternative interpretation for these differences might be the randomness of clone selection and the initial concentrations of the templates in the amplication reactions. 37 In addition, members of the genera Sulfurospirillum, Acinetobacter, Arcobacter, Bacteroides and Aerococcus were abundant in HT samples prior to other samples at both DNA and RNA level ( Fig.…”

Section: Diversity Of Microbial Communitiesmentioning

confidence: 99%

Unravelling diversity and metabolic potential of microbial consortia at each stage of leather sewage treatment

Liang

2017

RSC Adv.

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Section: Diversity Of Microbial Communitiesmentioning

confidence: 99%

Unravelling diversity and metabolic potential of microbial consortia at each stage of leather sewage treatment

Liang

2017

RSC Adv.

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“…In this process, a large fraction of the organic matter, which is degraded by a complex community of microorganisms, is broken down into carbon dioxide (CO2) and methane (CH4). Such decomposition occurs in the absence of oxygen and two main final products can be distinguished: digestate and biogas, which is considered a product of high added value to be used as biofuel in a sustainable and environmentally friendly way (4). After the anaerobic digestion, the digestate can be treated to remove liquid fraction and simultaneously concentrate nutrients, so a solid-liquid phase separation is usually carried out prior to any further post-treatment.…”

Section: Introductionmentioning

confidence: 99%

Impact of anaerobic digestion and centrifugation/decanting processes in bacterial communities fractions

Díaz

Oulego

Collado

et al. 2018

Journal of Bioscience and Bioengineering

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Sewage sludge can be treated by anaerobic processes that frequently are followed by physical separation processes. In this work, a high-throughput sequencing technology, based on variation in the bacterial 16S rRNA gene, has been used to characterise the bacterial populations present in samples taken from different points of an industrial anaerobic digestion process fed with sewage sludge. Relative abundances of phyla and classes throughout the biological process and the subsequent separation steps were determined. Results revealed that the Bacteroidetes, Firmicutes and Proteobacteria phyla were the most representative. However, significant changes in relative abundance were detected along treatments, showing the influence of operational parameters on the distribution of microorganisms throughout the process. After anaerobic digestion, phylum Firmicutes doubled its relative abundance, which seems to indicate that the anaerobic conditions and the nutrients favoured its growth, in contrast to other phyla that almost disappeared. After centrifugation, Proteobacteria went preferentially to the solid phase, in contrast to Firmicutes which was the dominant phylum in the liquid phase. After decanting the liquid phase during 45 h, an important growth of Proteobacteria, Spirochaetes and Tenericutes was detected. At class level, only significantly changes were observed for Proteobacteria classes being α-proteobacteria dominant in the digestate, while γ-proteobacteria was the majority since this point to the final steps. To know the changes on the kind and abundance of microbial populations throughout the anaerobic and separation processes is very important to understand how the facilities design and operation conditions can influence over the efficiencies of next biological treatments.

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“…Different substrates in the influent water. The different substrates in the influent could cause differences in bacterial composition [10,20,40]. Pre-chlorinated water and ozonated water flowed into the three rapid filters and the three BAC filters, respectively.…”

Section: Factors Influencing the Bacterial Community Structure Of Biomentioning

confidence: 99%

“…Recently, many researchers have used molecular biological methods to evaluate microbial community structures in the aquatic ecosystem. In particular, next-generation sequencing (NGS) is a culture independent and high-throughput method of analyzing the structure of an entire microbial community [10,19,20]. In addition, community-level physiological profiling (CLPP) based on sole-carbon-source utilization (SCSU) patterns has deepened understanding of the metabolic ability and functional characteristics of microbial communities [21].…”

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Characteristics of Bacterial Communities in Biological Filters of Full-Scale Drinking Water Treatment Plants

Choi¹,

Cha²,

Kim³

2019

Journal of Microbiology and Biotechnology

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Biological filtration refers to the process of removing both particulate matter and biodegradable organic matter (BOM) from water [1]. Biological filters can remove organic compounds through the fixed biofilm that develops on various media such as sand, anthracite, granular activated carbon (GAC), or membranes [2]. The biologically active rapid filter and the biological activated carbon (BAC) filter have been widely used for decades in drinking water treatment plants [1-3]. The rapid filter is usually filled with sand or anthracite, and is considered the most economical way to remove particles and BOM with the same filter unit [1]. However, many previous studies have demonstrated that the BAC filter is a more appropriate process to remove BOM than the rapid filter [4, 5]. Generally, the combination of ozonation and GAC filter is referred to as the BAC process [3]. Ozonation converts high-molecular-weight refractory organic matter into low-molecular-weight BOM, and this increased BOM can be effectively removed by the bacteria attached to the BAC filter [1, 4, 6-8]. Biological filtration has many advantages for drinking water treatments. First of all, it is important to increase the biostability of drinking water by reducing the quantity of BOM which can cause bacterial regrowth in distribution systems [1, 3-5]. It can reduce the disinfection by-product

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Microbial community structure in a full-scale anaerobic treatment plant during start-up and first year of operation revealed by high-throughput 16S rRNA gene amplicon sequencing

Cited by 53 publications

References 36 publications

Unravelling diversity and metabolic potential of microbial consortia at each stage of leather sewage treatment

Unravelling diversity and metabolic potential of microbial consortia at each stage of leather sewage treatment

Impact of anaerobic digestion and centrifugation/decanting processes in bacterial communities fractions

Characteristics of Bacterial Communities in Biological Filters of Full-Scale Drinking Water Treatment Plants

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