Microbial community structure associated with the high loading anaerobic codigestion of olive mill and abattoir wastewaters

Gannoun, Hana; Omri, Ilhem; Chouari, Rakia; Khelifi, Eltaief; Keskes, Sajiâa; Godon, Jean‐Jacques; Hamdi, Moktar; Sghir, Abdelghani; Bouallagui, Hassib

doi:10.1016/j.biortech.2015.11.050

Cited by 31 publications

(10 citation statements)

References 32 publications

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“…The overall process presented high tolerance toward the increase of OLR and the reduction of HRT without significant inhibition phenomena. The results obtained in the present study are in agreement with data reported by Gannoun et al for stable COD removal efficiency of OMWW and abattoir digestion, between 70 and 72%, when OLR increased from 3 to 6 g COD L −1 per day and with an HRT of 6.7 days.…”

Section: Resultssupporting

confidence: 93%

“…In the past, different nitrogen‐rich substrates have been utilized for the management of OMWW. These included the poultry litter, cattle manure, sewage sludge, swine manure, and abattoir wastes . Moreover, a number of other researchers assessed the effect of the addition of bioavailable nutrients and micronutrients in concentrated forms to the process and in this way improved the overall stability …”

Section: Introductionmentioning

confidence: 99%

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Anaerobic Digestion of Olive Mill Wastewater: Focusing on the Effect of Nitrogen Source

Zarkadas

Zachariou

Stamou

et al. 2019

CLEAN Soil Air Water

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Olive oil production is a seasonal agricultural activity taking place around the Mediterranean Sea with high financial importance and significant environmental footprint. Unfortunately, olive milling is generating olive mill wastewater (OMWW), which is an effluent with high carbon-to-nitrogen ratio and low pH. The aim of this work is to assess the application of different high nitrogen substrates for overcoming the problem of nitrogen deficiency of the mono-digestion of olive mill wastewater. The nitrogen-rich substrates are fish meal, bone, and meat meal (BMM), blood meal (BM), feather meal (FEM), mixtures of FEM and BM, soybean meal, and corn-gluten meal, which are mixed with the olive mill wastewater in order to attain C/N ¼ 20:1. The substrates are evaluated in batch and tank reactors. According to the results, the type of protein is an insignificant process parameter and the process can be developed without inhibition phenomena to be presented with any of the co-substrates assessed. The maximum bio-methane potential registered for the mixture of olive mill wastewater and bone, and meat meal with 449 mL CH 4 g À1 volatile solids added (VS) with an organic loading rate (OLR) of 3.6 kg V À1 s À1 m À3 per day. The overall process presented remarkable tolerance during the stepwise increase of OLR from 3.6 to 5.8 kg V À1 s À1 m À3 per day with the efficiency of the process to be reduced by 15% for all co-digestion mixtures while the volumetric production increased from 1.5 to 2.1 m 3 CH 4 m À3 per day.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Anaerobic Digestion of Olive Mill Wastewater: Focusing on the Effect of Nitrogen Source

Zarkadas

Zachariou

Stamou

et al. 2019

CLEAN Soil Air Water

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“…Interestingly, we have previously reported a concurrence between increasing amounts of Actinobacteria and low methane production [8]. Occurrence of Actinobacteria in anaerobic digester plants has been reported repeatedly [46, 47]. Actinobacteria were described in previous works as important key players in the degradation of plant material in compost [48] with effective enzymes that can allow large-scale application for breakdown of cellulosic plant material [49].…”

Section: Resultsmentioning

confidence: 91%

From grass to gas: microbiome dynamics of grass biomass acidification under mesophilic and thermophilic temperatures

Abendroth

Simeonov²,

Peretó

et al. 2017

Biotechnol Biofuels

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BackgroundSeparating acidification and methanogenic steps in anaerobic digestion processes can help to optimize the process and contribute to producing valuable sub-products such as methane, hydrogen and organic acids. However, the full potential of this technology has not been fully explored yet. To assess the underlying fermentation process in more detail, a combination of high-throughput sequencing and proteomics on the acidification step of plant material (grass) at both mesophilic and thermophilic temperatures (37 and 55 °C, respectively) was applied for the first time.ResultsHigh-strength liquor from acidified grass biomass exhibited a low biodiversity, which differed greatly depending on temperature. It was dominated by Bacteroidetes and Firmicutes at 37 °C, and by Firmicutes and Proteobacteria at 55 °C. At the methane stage, Methanosaeta, Methanomicrobium and Methanosarcina proved to be highly sensitive to environmental changes as their abundance in the seed sludges dropped dramatically after transferring the seed sludges from the respective reactors into the experimental setup. Further, an increase in Actinobacteria coincided with reduced biogas production at the end of the experiment. Over 1700 proteins were quantified from the first cycle of acidification samples using label-free quantitative proteome analysis and searching protein databases. The most abundant proteins included an almost complete set of glycolytic enzymes indicating that the microbial population is basically engaged in the degradation and catabolism of sugars. Differences in protein abundances clearly separated samples into two clusters corresponding to culture temperature. More differentially expressed proteins were found under mesophilic (120) than thermophilic (5) conditions.ConclusionOur results are the first multi-omics characterisation of a two-stage biogas production system with separated acidification and suggest that screening approaches targeting specific taxa such as Methanosaeta, Methanomicrobium and Methanosarcina could be useful diagnostic tools as indicators of environmental changes such as temperature or oxidative stress or, as in the case of Actinobacteria, they could be used as a proxy of the gas production potential of anaerobic digesters. Metaproteome analyses only detected significant expression differences in mesophilic samples, whereas thermophilic samples showed more stable protein composition with an abundance of chaperones suggesting a role in protein stability under thermal stress.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0859-0) contains supplementary material, which is available to authorized users.

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“…In AD systems, thermodynamic equilibrium of the biochemical reactions (Wilson et al, 2008) and microbial community structure, activities and diversity (Gannoun et al, 2016;Gunnigle et al, 2015;Sun et al, 2015) can be influenced by temperature. For example, methanogens show higher growth rates at thermophilic condition, which may induce efficient methane production (Sun et al, 2015;Weiland, 2010).…”

Section: Introductionmentioning

confidence: 99%

Temperature regulates methane production through the function centralization of microbial community in anaerobic digestion

Lin

Vrieze

et al. 2016

Bioresource Technology

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Microbial community structure associated with the high loading anaerobic codigestion of olive mill and abattoir wastewaters

Cited by 31 publications

References 32 publications

Anaerobic Digestion of Olive Mill Wastewater: Focusing on the Effect of Nitrogen Source

Anaerobic Digestion of Olive Mill Wastewater: Focusing on the Effect of Nitrogen Source

From grass to gas: microbiome dynamics of grass biomass acidification under mesophilic and thermophilic temperatures

Temperature regulates methane production through the function centralization of microbial community in anaerobic digestion

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