“…Collectively these issues require complex bench workflows, requiring a high degree of molecular biological knowledge and skills, thus the interpretation of observed metatranscriptomics results should be performed with care and critical thinking, due to the complexity of organism's metabolism. However, even with the challenges, metatranscriptomics is used more and more for understanding microbial activity in different environments under influence of particular conditions and also is widely used to analyze WWTP microbial communities (Maza-Márquez et al, 2022;McDaniel et al, 2021b;Moran et al, 2013;Rodríguez et al, 2015;Spurbeck et al, 2023;Wang et al, 2018). To get even deeper insight into the microbial communities activity, multiple -omics could be coupled with metabolomics or proteomics to obtain a comprehensive analysis of all metabolites of a sample that are identified and quantified (Aguiar-Pulido et al, 2016).…”