Microbial community in the rhizosphere of young maize seedlings is susceptible to the impact of introduced pseudomonads as indicated by FAME analysis

Abstract

Two species of Pseudomonas (i.e. P. chlororaphis or P. putida) derived from a maize rhizosphere were studied for their impact on the structure of the microbial community in the rhizosphere of young maize seedlings after inoculation. The culturable bacteria and total microbial communities were analyzed based on profiles of whole-cell fatty acid methyl esters (MIDI-FAME). The introduction of Pseudomonas species resulted in the shift from the Gram-positive dominated culturable community in the rhizosphere of uninoculated maize to more Gram-negative populations in the rhizospheres of the inoculated plants. For the total rhizosphere communities, 43, 47 and 42 FAMEs were detected in the uninoculated maize and the samples inoculated with P. chlororaphis or P. putida, respectively. In contrast to the culturable communities, low concentrations of marker FAMEs for Gram-positives (i15:0, a15:0, i16:0) were found in the profiles of the total rhizosphere communities. The maize inoculations resulted in an enrichment of some Gram-negative isolates; however, Gram-positive bacteria, Cytophaga/Flavobacterium and saprophytic fungi were found in the uninoculated rhizosphere.

Significance of Silver Birch and Bushgrass for Establishment of Microbial Heterotrophic Community in a Metal-Mine Spoil Heap

“…This discrepancy between results of direct extraction of fatty acids from soil and microbial colonies cultured on agar media has been reported in several studies (Bååth et al, 2005;Ibekwe & Kennedy, 1998;Kozdrój, 2008). Mostly Gram-negative bacteria may have predominated over the total community of soil bacteria in the heap sites, as indicated by high concentrations of monounsaturated fatty acids and presence of hydroxy and cyclopropane FAMEs.…”

Diversity and metabolic potential of culturable bacteria from the rhizosphere of Turkish tea grown in acidic soils

“…These bacteria are often identified as dominant taxa of cultivable microbial populations from rhizosphere of various crop plants (Aravind et al 2009;Caesar-TonThat et al 2007;Çakmakçi et al 2006;Germida and Siciliano 2001;Kozdrój 2008;Park et al 2005;Rusznyák et al 2008;Vessey 2003). Among the Gram-positive bacteria identified by FAME, species of Bacillus, Brevibacillus, Paenibacillus and Arthobacter occurred in both barley and alfalfa soil (Caesar-TonThat et al 2007).…”

“…The field of rhizosphere microbiology of tea garden soil ecosystems is unexplored and in this work, we have isolated the bacterial population from the rhizosphere of tea plants production zones of various agro climatic regions in Rize and Trabzon during June-September, 2007-2008. The study area is located on the eastern Black Sea region, most in Rize, between 40°50′ and41°20′ N and38°49′ and41°28′ E. The tea gardens were established between 1938-1944 years and now all of them are between 65-70 years old.…”

Inoculation With the Plant-Growth-Promoting Rhizobacterium Pseudomonas fluorescens LBUM677 Impacts the Rhizosphere Microbiome of Three Oilseed Crops

“…However, there have also been studies that have shown a direct effect on the plant microbiome due to bacterial inoculations. Examples include inoculations of Rhizobium species in faba beans ( Vicia faba L.) ( Zhang et al, 2010 ) and soybean ( Glycine max L.) ( Zhang et al, 2011 ), Proteus vulgaris in Kimchi cabbage ( Brassica rapa L.) ( Bhattacharyya and Lee, 2016 ), and Pseudomonas species in maize ( Kozdrój et al, 2004 ; Kozdrój, 2008 ). The advent of metagenomic sequencing to examine the effects of microbial inoculation on the non-target soil microbiome will be a research area that will see great developments in the near future ( Martínez-Hidalgo et al, 2019 ).…”