Microbial community functional structure in response to antibiotics in pharmaceutical wastewater treatment systems

Abstract

a b s t r a c tIt is widely demonstrated that antibiotics in the environment affect microbial community structure. However, direct evidence regarding the impacts of antibiotics on microbial functional structures in wastewater treatment systems is limited. Herein, a highthroughput functional gene array (GeoChip 3.0) in combination with quantitative PCR and clone libraries were used to evaluate the microbial functional structures in two biological wastewater treatment systems, which treat antibiotic production wastewater mainly containing oxytetracycline. Despite the bacteriostatic effects of antibiotics, the GeoChip detected almost all key functional gene categories, including carbon cycling, nitrogen cycling, etc., suggesting that these microbial communities were functionally diverse. Totally 749 carbon-degrading genes belonging to 40 groups (24 from bacteria and 16 from fungi) were detected. The abundance of several fungal carbon-degrading genes (e.g., glyoxal oxidase ( glx), lignin peroxidase or ligninase (lip), manganese peroxidase (mnp), endochitinase, exoglucanase_genes) was significantly correlated with antibiotic concentrations (Mantel test; P < 0.05), showing that the fungal functional genes have been enhanced by the presence of antibiotics. However, from the fact that the majority of carbon-degrading genes were derived from bacteria and diverse antibiotic resistance genes were detected in bacteria, it was assumed that many bacteria could survive in the envi- Available online at www.sciencedirect.com journal homepage: www.elsevier.com/locate /wa tres w a t e r r e s e a r c h 4 7 ( 2 0 1 3 ) 6 2 9 8 e6 3 0 8

Performance and Yeast Tracking in A Full-Scale Oil-Containing Paromomycin Production Wastewater Treatment System Using Yeast

“…In our study, the fungal/bacterial gene copy ratio was 14.87, much higher than that observed in streptomycin and oxytetracycline production wastewater treatment activated sludge systems, and significantly higher than non-antibiotic (inosine) fermentation wastewater treatment plants [21,33] (Table 5), suggesting successful yeast colonization. Though further study is required, the high antibiotic residue in production waste mother liquor might have strongly affected the microbial community structure.…”

“…The specific methods for clone libraries were based on our previous study [21]. Online sequence similarity searching was performed using the NCBI BLAST program and GenBank database.…”

“…Oil-rich wastewater treated by yeast is released mainly from slaughterhouses and tanneries, as well as dairy and oil food processing industries, but little information is available regarding the treatment of oil-containing antibiotic production wastewater by yeast. Our previous study revealed for the first time that many key functional genes of fungi involved in carbon degradation in activated sludge are enhanced under high levels of antibiotics [21], showing the considerable potential of yeast for the pretreatment of antibiotic production wastewater. It is speculated, therefore, that yeast technology could be used in the pretreatment of oil-containing fermentation antibiotic production wastewater.…”

Microbial community structure and function of Nitrobenzene reduction biocathode in response to carbon source switchover

“…Moreover, the clear microbial communities succession from the initial NB-reducing inoculum to the NB-reducing biocathode biofilms, and microbial functional gene diversity, especially putative functional genes related to electrons transfer that in response to carbon source switchover (from organic glucose to inorganic bicarbonate) using high-throughput metagenomics technology have not yet been understood. Illumina MiSeq sequencing platform (Caporaso et al, 2012) and GeoChip-based functional gene arrays Liu et al, 2010;Zhang et al, 2013;Zhou et al, 2013) are suitable tools to characterize microbial community structure, function and metabolic potential in NBreducing biocathode biofilms.…”

Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

“…Recently, GeoChip 3.0 and the GeoChip 4 series have been applied to the investigations of industrial WWTPs (Zhang et al 2013) and municipal wastewater treatment systems (Sun et al 2014;Wang et al 2014), respectively. The studies successfully revealed some linkages between functional potential and environmental parameters.…”