Microbial Community Dynamics during a Harmful Chrysochromulina leadbeateri Bloom in Northern Norway

Aalto, Nerea Johanna; Schweitzer, Hannah; Grann-Meyer, Erlend; Krsmanovic, Stina; Svenning, Jon Brage; Dalheim, Lars; Petters, Sebastian; Ingebrigtsen, Richard Andre; Hulatt, Chris J.; Bernstein, Hans C.

doi:10.1128/aem.01895-22

Cited by 1 publication

(3 citation statements)

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“…The majority of taxonomic recruitment was observed within the prokaryotic portion of the microbiome but observations were also made with respect to microeukaryotic taxa. Changes were observed on the time scale of days, in a similar fashion to previous observations from temperate and coastal cold-water microbial ecosystems [36,44]. It is expected that the diverse community comprises members with different ecological properties, which potentially have a strong impact on the function of the production system in relation to CCU [15].…”

Section: Ccu Bioreactors Are Unique Industrial Marine Microbial Ecosy...supporting

confidence: 84%

“…Bacteria that are commonly associated with marine diatoms are known to utilize different alga-derived organic substrates – i.e., DOC that is released across different bloom or growth stages or generated through temporal substrate degradation [44, 47]. These dynamic processes at least partially underpin the successional blooming patterns of the prokaryotic groups that were identified in this study and other investigations [36, 45]. The orders Opitutales ( Verrumicrobiota ) and Chitinophagales which also belonged to the most abundant community ASVs from the current study are not considered either uncommon or specific to our production system as they have also been documented in environmental and experimental studies.…”

Section: Discussionmentioning

confidence: 85%

“…The K-medoids clustering method was depolyed following the previously described approach applied to microbiome time series data [35, 36]. It was used to investigate shifts in microbiome structure by examining community members that share similar succession patterns across the crash and healthy time series.…”

Section: Methodsmentioning

confidence: 99%

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The bioreactor microbiome of mass cultivated marine diatoms for industrial carbon capture and utilization

Aalto,

Giæver,

Eriksen

et al. 2023

Preprint

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Marine microalgae are a promising innovation platform for carbon capture and utilization (CCU) biotechnologies to mitigate industrial greenhouse gas emissions. However, industrial-scale cultivation of algal mono-cultures is challenging and often unscalable. Non-axenic microalgae in large semi-open CCU photobioreactors leads to the co-cultivation of diverse microbial communities. There is limited knowledge about the "bioreactor ecology" involving microalgae interacting with the microbiome and its subsequent impact on stability and productivity. In this study, we describe the semi-continuous industrial mass cultivation of the cold-adapted marine diatom,Porosira glacialisUiT201, by investigating the prokaryotic and microeukaryotic communities. Data were collected in two consecutive time series experiments, representing the initiation and operation of an preindustrial scale CCU photobioreactor (300,000 liters). The first experiment experienced a culture "crash" of the focal strain after 39 days, while the second culture remained stable and "healthy" for 60 days. The results highlight that this mass cultivation system represents a unique industrial marine microbial ecosystem. The succession of the prokaryotic community was primarily driven by species replacement, indicating turnover due to selective bioreactor conditions and/or biological interactions. Nonetheless, the bioreactor consistently harbors a recurring and abundant core microbiome, suggesting that the closely associated bacterial community is influenced by microalgae-specific properties and can endure a dynamic and variable environment. The observed culture collapse ofP. glacialiscoincided with changes in the core microbiome structure and different environmental growth conditions compared to the stable and "healthy" experiment. These findings imply that cohabiting microbial taxa within industrial microalgae cultivation likely play a critical role in stabilizing the conversion of industrial CO2into marine biomass, and changes in community structure serve as an indicator of process stability.

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Section: Ccu Bioreactors Are Unique Industrial Marine Microbial Ecosy...supporting

confidence: 84%

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

The bioreactor microbiome of mass cultivated marine diatoms for industrial carbon capture and utilization

Aalto,

Giæver,

Eriksen

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Microbial Community Dynamics during a Harmful Chrysochromulina leadbeateri Bloom in Northern Norway

Cited by 1 publication

References 50 publications

The bioreactor microbiome of mass cultivated marine diatoms for industrial carbon capture and utilization

The bioreactor microbiome of mass cultivated marine diatoms for industrial carbon capture and utilization

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