Microbial Community Drivers in Anaerobic Granulation at High Salinity

Gagliano, Maria Cristina; Sudmalis, Dainis; Pei, Ruizhe; Temmink, Hardy; Plugge, Caroline M.

doi:10.3389/fmicb.2020.00235

Cited by 31 publications

(22 citation statements)

References 87 publications

(111 reference statements)

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“…It is, of course, interesting to observe that granules grew in this experiment without the apparent dominant involvement of the filamentous Methanosaeta (Methanothrix), which tends to contradict the conventional understanding of granulation microbiology (Weigant, 1987;Hulshoff Pol et al, 2004). Other filamentous bacteria, however, were present such as Streptococcus, which was recently linked to granulation in high-salinity wastewaters (Gagliano et al, 2020).…”

Section: Emerging Pathways: Dominance Of Hydrogenotrophic Methanogenesismentioning

confidence: 76%

“…These archaea can either (i) aggregate together, (ii) attach to suspended particles, or (iii) potentially form a bridge between existing microflocs-aiding in the critical first step of forming granule precursors (Dubourgier et al, 1987;Weigant, 1987;Jian and Shi-yi, 1993). In fact, recent studies still confirm the importance of Methanosaeta, even at high salinity (Gagliano et al, 2020).…”

Section: Introductionmentioning

confidence: 98%

“…Granulation is a process whereby suspended particles and planktonic cells accumulate, forming small dense biofilm aggregates (Liu et al, 2002;Show et al, 2020). Unlike conventional biofilm formation, which is a well-documented phenomenon (O'Toole et al, 2000;Hall-Stoodley et al, 2004), the specific mechanisms involved in anaerobic sludge granulation are still being teased-out (Sanjeevi et al, 2013;Kim et al, 2014;Gagliano et al, 2017Gagliano et al, , 2020Sudmalis et al, 2018;Faria et al, 2019). The topic has been comprehensively reviewed, and the various theories summarized, which can be categorized as physical, microbial, or thermodynamic (Hulshoff Pol et al, 2004;Show et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Growth and Break-Up of Methanogenic Granules Suggests Mechanisms for Biofilm and Community Development

et al. 2020

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Methanogenic sludge granules are densely packed, small, spherical biofilms found in anaerobic digesters used to treat industrial wastewaters, where they underpin efficient organic waste conversion and biogas production. Each granule theoretically houses representative microorganisms from all of the trophic groups implicated in the successive and interdependent reactions of the anaerobic digestion (AD) process. Information on exactly how methanogenic granules develop, and their eventual fate will be important for precision management of environmental biotechnologies. Granules from a full-scale bioreactor were size-separated into small (0.6-1 mm), medium (1-1.4 mm), and large (1.4-1.8 mm) size fractions. Twelve laboratory-scale bioreactors were operated using either small, medium, or large granules, or unfractionated sludge. After >50 days of operation, the granule size distribution in each of the small, medium, and large bioreactor sets had diversified beyond-to both bigger and smaller than-the size fraction used for inoculation. Interestingly, extra-small (XS; <0.6 mm) granules were observed, and retained in all of the bioreactors, suggesting the continuous nature of granulation, and/or the breakage of larger granules into XS bits. Moreover, evidence suggested that even granules with small diameters could break. "New" granules from each emerging size were analyzed by studying community structure based on high-throughput 16S rRNA gene sequencing. Methanobacterium, Aminobacterium, Propionibacteriaceae, and Desulfovibrio represented the majority of the community in new granules. H2-using, and not acetoclastic, methanogens appeared more important, and were associated with abundant syntrophic bacteria. Multivariate integration (MINT) analyses identified distinct discriminant taxa responsible for shaping the microbial communities in different-sized granules.

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Section: Emerging Pathways: Dominance Of Hydrogenotrophic Methanogenesismentioning

confidence: 76%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Growth and Break-Up of Methanogenic Granules Suggests Mechanisms for Biofilm and Community Development

et al. 2020

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“…Methanobacterium genus (17.24%) was secondly dominant. Methanobacterium is a strict hydrogenotrophic methanogen using hydrogen and carbon dioxide to form methane [46] Methanomicrobiales (14.29%) was thirdly dominant. Subsequently, Methanomassiliicoccoceae (8.26%) and Methanosarcina (6.87%) were abundant.…”

Section: Top Archaea In Genus Level In Cake Layermentioning

confidence: 99%

“…Subsequently, Methanomassiliicoccoceae (8.26%) and Methanosarcina (6.87%) were abundant. The genus Methanosarcina performs acetoclastic, hydrogenotrophic, and methylotrophic methanogenesis [46]. Figure 4 shows the presence of different bacteria phyla in the bulk sludge, cake layer and the cleaning solutions.…”

Section: Top Archaea In Genus Level In Cake Layermentioning

confidence: 99%

New Insights into the Microbial Diversity of Cake Layer in Yttria Composite Ceramic Tubular Membrane in an Anaerobic Membrane Bioreactor (AnMBR)

Nilusha

Wei

2021

Membranes

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Cake layer formation is an inevitable challenge in membrane bioreactor (MBR) operation. The investigations on the cake layer microbial community are essential to control biofouling. This work studied the bacterial and archaeal communities in the cake layer, the anaerobic sludge, and the membrane cleaning solutions of anaerobic membrane bioreactor (AnMBR) with yttria-based ceramic tubular membrane by polymerase chain reaction (PCR) amplification of 16S rRNA genes. The cake layer resistance was 69% of the total membrane resistance. Proteins and soluble microbial by-products (SMPs) were the dominant foulants in the cake layer. The pioneering archaeal and bacteria in the cake layer were mostly similar to those in the anaerobic bulk sludge. The dominant biofouling bacteria were Proteobacteria, Bacteroidetes, Firmicutes, and Chloroflexi and the dominant archaeal were Methanosaetacea and Methanobacteriacea at family level. This finding may help to develop antifouling membranes for AnMBR treating domestic wastewater.

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