Microbial community diversity and composition across a gradient of soil acidity in spruce–fir forests of the southern Appalachian Mountains

Bardhan, Sougata; Jose, Shibu; Jenkins, Michael A.; Webster, Christopher R.; Udawatta, Ranjith P.; Stehn, Sarah E.

doi:10.1016/j.apsoil.2012.04.010

Cited by 45 publications

(20 citation statements)

References 49 publications

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“…Apparently, the level of nitrogen input is negatively correlated with the relative abundance of Acidobacteria ( Fierer et al, 2012 ), which is line with the results of our study. Acidobacteria belonged to the acidophilic bacteria, and Acidobacteria abundance is significantly higher under acidic conditions ( Dion, 2008 ; Lauber et al, 2009 ; Bardhan et al, 2012 ). However, in our study, the relative abundance of the Acidobacteria was not correlated with soil pH (Table 5 ) but was significantly negatively correlated with AP.…”

Section: Discussionmentioning

confidence: 99%

Variations in Soil Bacterial Community Diversity and Structures Among Different Revegetation Types in the Baishilazi Nature Reserve

et al. 2018

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We compared patterns of soil bacterial community diversity and structure in six secondary forests (JM, Juglans mandshurica; QM, Quercus mongolica; MB, mixed Broadleaf forest; BE, Betula ermanii; CB, conifer-broadleaf forest; PT, Pinus tabuliformis) and two plantation forests (LG, Larix gmelinii; PK, Pinus koraiensis) of the Baishilazi Nature Reserve, China, based on the 16S rRNA high-throughput Illumina sequencing data. The correlations between the bacterial community and soil environmental factors were also examined. The results showed that the broadleaf forests (JM, QM, MB) had higher levels of total C (TC), total N (TN), available N (AN), and available K (AK) compared to the coniferous forests (PT, LG, PK) and conifer-broadleaf forest (CB). Different revegetation pathways had different effects on the soil bacterial community diversity and structure. For the α-diversity, the highest Shannon index and Simpson index were found in JM. The Simpson index was significantly positively correlated with the available P (AP) (P < 0.05), and the Shannon index was significantly positively correlated with AK (P < 0.05). Compared with others, the increased ACE index and Chao1 index were observed in the CB and MB, and both of these α-diversity were significantly negative with AK (P < 0.05). The relative abundances of bacterial phyla and genera differed among different revegetation types. At the phylum level, the dominant phylum groups in all soils were Proteobacteria, Acidobacteria, Actinobacteria, Verrucomicrobia, Chloroflexi, Bacteroidetes, Gemmatimonadetes, and Planctomycetes. Significant differences in relative abundance of bacteria phyla were found for Acidobacteria, Actinobacteria, Chloroflexi, Gemmatimonadetes, and Proteobacteria. Correlation analysis showed that Soil pH, TC, TN, AP, and AK were the main abiotic factors structuring the bacterial communities. As revealed by the clear differentiation of bacterial communities and the clustering in the heatmap and in the PCA plots, broadleaf forests and coniferous forests harbored distinct bacterial communities, indicating a significant impact of the respective reforestation pathway on soil bacterial communities in the Baishilazi Nature Reserve.

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Section: Discussionmentioning

confidence: 99%

Variations in Soil Bacterial Community Diversity and Structures Among Different Revegetation Types in the Baishilazi Nature Reserve

et al. 2018

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“…Actinobacteria have often been cited as a major phylum composing soil bacterial communities [48,[52][53][54], as they have been primarily linked to C cycling and the breakdown of organic matter [43,46]. The nutrients provided in relation to this process are evident in the WSS, though Actinobacteria abundance is at its lowest in this area.…”

Section: Bacterial Community Membershipmentioning

confidence: 98%

Assessing the Diversity and Composition of Bacterial Communities across a Wetland, Transition, Upland Gradient in Macon County Alabama

et al. 2013

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Wetlands provide essential functions to the ecosphere that range from water filtration to flood control. Current methods of evaluating the quality of wetlands include assessing vegetation, soil type, and period of inundation. With recent advances in molecular and bioinformatic techniques, measurement of the structure and composition of soil bacterial communities have become an alternative to traditional methods of ecological assessment. The objective of the current study was to determine whether soil bacterial community composition and structure changed along a single transect in Macon County, AL. Proteobacteria were the most abundant phyla throughout the soils in the study (ranging from 42.1% to 49.9% of total sequences). Phyla Acidobacteria (37.4%) and Verrucomicrobia (7.0%) were highest in wetland soils, Actinobacteria (14.6%) was highest in the transition area, and Chloroflexi (1.6%) was highest in upland soils. Principle Components Analysis (relative abundance) and Principle Coordinates Analysis (PCoA) (Unifrac weighted metric) plots were generated, showing distinction amongst the OPEN ACCESSDiversity 2013, 5 462 ecosystem types through clustering by taxonomic abundance and Unifrac scores at 3% dissimilarity, respectively. Selected soil properties (soil organic carbon and phosphatase enzyme activity) also differed significantly in transition soil ecosystem types, while showing predominance in the wetland area. This study suggests that with further study the structure and composition of soil bacterial communities may eventually be an important indicator of ecological impact in wetland ecosystems.

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“…However, studies on soil microbial diversity over altitudinal gradients indicate that these patterns cannot be applied for microorganisms and contradictory conclusions have been drawn from different works [ 6 ]. For example, no significant differences were found in soil bacterial diversity, using pyrosequencing, along altitudinal gradients in eastern Peru, Appalachian Mountains, and southwestern highlands of Saudi Arabia [ 5 , 7 , 8 ]. Meng et al [ 4 ] found the highest bacterial diversity at medium elevations in the Mountain Lushan, China, while Singh et al [ 9 ] found a higher bacterial diversity at higher altitudes than at medium altitudes on Mt.…”

Section: Introductionmentioning

confidence: 99%

Abundance and Diversity of Bacterial, Archaeal, and Fungal Communities Along an Altitudinal Gradient in Alpine Forest Soils: What Are the Driving Factors?

2016

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Shifts in soil microbial communities over altitudinal gradients and the driving factors are poorly studied. Their elucidation is indispensable to gain a comprehensive understanding of the response of ecosystems to global climate change. Here, we investigated soil archaeal, bacterial, and fungal communities at four Alpine forest sites representing a climosequence, over an altitudinal gradient from 545 to 2000 m above sea level (asl), regarding abundance and diversity by using qPCR and Illumina sequencing, respectively. Archaeal community was dominated by Thaumarchaeota, and no significant shifts were detected in abundance or community composition with altitude. The relative bacterial abundance increased at higher altitudes, which was related to increasing levels of soil organic matter and nutrients with altitude. Shifts in bacterial richness and diversity as well as community structure (comprised basically of Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes) significantly correlated with several environmental and soil chemical factors, especially soil pH. The site at the lowest altitude harbored the highest bacterial richness and diversity, although richness/diversity community properties did not show a monotonic decrease along the gradient. The relative size of fungal community also increased with altitude and its composition comprised Ascomycota, Basidiomycota, and Zygomycota. Changes in fungal richness/diversity and community structure were mainly governed by pH and C/N, respectively. The variation of the predominant bacterial and fungal classes over the altitudinal gradient was the result of the environmental and soil chemical factors prevailing at each site.Electronic supplementary materialThe online version of this article (doi:10.1007/s00248-016-0748-2) contains supplementary material, which is available to authorized users.

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Microbial community diversity and composition across a gradient of soil acidity in spruce–fir forests of the southern Appalachian Mountains

Cited by 45 publications

References 49 publications

Variations in Soil Bacterial Community Diversity and Structures Among Different Revegetation Types in the Baishilazi Nature Reserve

Variations in Soil Bacterial Community Diversity and Structures Among Different Revegetation Types in the Baishilazi Nature Reserve

Assessing the Diversity and Composition of Bacterial Communities across a Wetland, Transition, Upland Gradient in Macon County Alabama

Abundance and Diversity of Bacterial, Archaeal, and Fungal Communities Along an Altitudinal Gradient in Alpine Forest Soils: What Are the Driving Factors?

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