Microbial community change of sulfate reduction and sulfur oxidation bacteria in the activated sludge cultivated with acetate and peptone

Miyazato, Naoki; Yamamoto-Ikemoto, Ryoko; Takamatsu, Saori

doi:10.2166/wst.2006.856

Cited by 6 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3, Thiothrix was the main taxon affecting the settling properties. This is in agreement with previous studies where extreme filamentous bulking was provoked by Thiothrix (SVI>600 mg/L, Yamamoto-Ikemoto et al, 1991;Miyazato et al, 2006). As suggested by Fig.…”

Section: Sludge Settling Propertiessupporting

confidence: 93%

Short-sludge age EBPR process – Microbial and biochemical process characterisation during reactor start-up and operation

et al. 2016

View full text Add to dashboard Cite

The new paradigm for used water treatment suggests the use of short solid retention times (SRT) to minimize organic substrate mineralization and to maximize resource recovery. However, little is known about the microbes and the underlying biogeochemical mechanisms driving these short-SRT systems. In this paper, we report the start-up and operation of a short-SRT enhanced biological phosphorus removal (EBPR) system operated as a sequencing batch reactor (SBR) fed with preclarified municipal wastewater, which is supplemented with propionate. The microbial community was analysed via 16S rRNA amplicon sequencing. During start-up (SRT = 8 d), the EBPR was removing up to 99% of the influent phosphate and completely oxidized the incoming ammonia. Furthermore, the sludge showed excellent settling properties. However, once the SRT was shifted to 3.5 days nitrification was inhibited and bacteria of the Thiothrix taxon proliferated in the reactor, thereby leading to filamentous bulking (sludge volume index up to SVI = 1100 mL/g). Phosphorus removal deteriorated during this period, likely due to the out-competition of polyphosphate accumulating organisms (PAO) by sulphate reducing bacteria (SRB). Subsequently, SRB activity was suppressed by reducing the anaerobic SRT from 1.2 day to 0.68 day, with a consequent rapid SVI decrease to ∼200 ml/g. The short-SRT EBPR effectively removed phosphate and nitrification was mitigated at SRT = 3 days and oxygen levels ranging from 2 to 3 mg/L.

show abstract

Section: Sludge Settling Propertiessupporting

confidence: 93%

Short-sludge age EBPR process – Microbial and biochemical process characterisation during reactor start-up and operation

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The highest maximum specific sulfide generation activity in the presence of peptone is 3 times lower than the one in which acetate is used as electron donor with the same inoculum, demonstrating, once again, the superior efficiency of acetate as substrate. Miyazato and coworkers illustrated the existence of SRB in an activated sludge cultured with peptone with a biogenic activities between 7.2 and 19.2 mg SO 2− 4 g −1 d −1 which is comparable with the results obtained in this research work with the sediments of the facultative lagoon of the WWTP in Cuenca [16].…”

supporting

confidence: 87%

“…Practically all other inocula evaluated (with exception of the discharge sludge of the WWTP in Quito) present maximum specific sulfide production or sulfate reduction activities half the value obtained with the sediments of the natural lagoon which were 4. 16 d −1 ) with the sediments of the natural lagoon. This difference suggests that the microbial consortium present in the sediments of the natural lagoon do not have a microbial lactate oxidizing activity as efficient as in the case of the mentioned studies.…”

mentioning

confidence: 99%

Efecto del sustrato como donante de electrones durante la reducción microbiana de sulfato y sus posibles aplicaciones en el tratamiento biológico de drenaje ácido de minas

Andrade

Ochoa‐Herrera

2013

Av. Cienc. Ing. (Quito)

View full text Add to dashboard Cite

The uncontrolled release of acid mine drainage (AMD) characterized by elevated concentrations of dissolved metals, high levels of sulfate ions and low pH values threatens the quality of water resources nearby mining areas worldwide. The development of novel technologies based on the precipitation of metals as metal sulfides catalyzed by sulfate reducing bacteria constitute an important method for the bioremediation of AMD. The objective of this study was to evaluate in batch experiments the efficiency of different organic substrates such as acetate, lactate, ethanol and peptone as electron donors during the generation of biogenic sulfide by different microbial inocula and its possible applications in the bioremediation of AMD. The highest sulfide production activity was obtained with 2.5 g acetate-COD L −1 as substrate, 4000 mg SO −2 4 L −1 as electron acceptor and the sediments of an artificial lagoon as bacterial inoculum. The final cumulative sulfide production was 463 mg S 2− L −1 , the maximum specific sulfide production activity was 9 mg S 2− g acetate −1 d −1 , and the maximum specific sulfate reduction activity was 52 mg SO −2 4 g acetate −1 d −1 . In terms of the substrate concentration, a 10-fold increase in the concentration of the electron donor resulted in substrate inhibition significantly decreasing the biogenic sulfide activities. The results of this study indicate that acetate was a highly effective substrate during the microbial sulfate reduction with a potential application in the remediation of acid mine drainage. Moreover, the use of acetate as electron donor favored the sulfate reducing activity through the inhibition of the methanogenic activity of the microorganisms present in the evaluated microbial inocula.Keywords. sulfate reducing bacteria (SRB), acid mine drainage (AMD), organic substrates, electron donor, biogenic sulfide activity. ResumenLa descarga no controlada de los drenajes ácidos de mina (DAM) caracterizados por presentar elevadas concentraciones de metales disueltos, iones sulfato y pH ácido amenaza la calidad de los cuerpos de agua cercanos a las zonas mineras alrededor del mundo. Tecnologías basadas en la precipitación de metales en forma de sulfuros metálicos, catalizada por las bacterias sulfato reductoras encargadas de la producción desasimilatoria de sulfuros constituyen un importante método de remediación de los DAM. En este trabajo de investigación se evaluó la eficiencia de diferentes sustratos tales como acetato, lactato, etanol y peptona como donadores de electrones durante la sulfato reducción microbiana. Diferentes inóculos microbianos y sus posibles aplicaciones en el tratamiento biológico de los DAM fueron estudiados en experimentos batch. La mayor eficiencia de producción de sulfuro catalizada por las bacterias sulfato reductoras fue alcanzada empleando 2.5 g DQO-acetato L −1 como donador de electrones, 4000 mg SO 2− 4 L −1 como aceptor de electrones y los sedimentos de una laguna artificial como inóculo bacteriano. La producción final acumulada de su...

show abstract

“…Mixed liquor suspended solids (MLSS) and sludge volume index (SVI) of the activated sludge collected from AN2 were measured. Batch experiments were conducted to examine the sulfur oxidizing rate and sulfate reducing rate according to Miyazato et al [9]. In the sulfur oxidizing batch test, the activated sludge was centrifuged, and we adjusted the MLSS concentration by adding substrate.…”

Section: Sampling In Wwtpsmentioning

confidence: 99%

“…It was reported that sulfate reduction is the main trigger for the growth of filamentous sulfur bacteria in the AO process [8,9]. Moreover, Yamamoto-Ikemoto et al conducted batch experiments using activated sludge from a municipal WWTP and reported that phosphorus release from the sludge, which had high sulfate reduction activity, tended to be lower than that of low-activity sludge [10].…”

Section: Introductionmentioning

confidence: 99%

Effects of Sulfates on Enhanced Biological Phosphorus Removal in Three Waste Water Treatment Plants

Kanazawa

Yamamoto-Ikemoto

Matsuura

2019

J. of Wat. & Envir. Tech.

View full text Add to dashboard Cite

The effects of sulfates on enhanced biological phosphorus removal (EBPR) were investigated in three municipal wastewater treatment plants (WWTPs), which treat sewage containing several concentrations of sulfates. The behaviour of sulfates and phosphates in the anaerobic oxic process was analysed by considering the daily inflow rate and water quality. Although phosphorus release and uptake were recognized in all plants, phosphorus release from the WWTPs that were treating high-sulfate wastewater (high-sulfate WWTP), was lower than that of the WWTP treating low-sulfate wastewater (lowsulfate WWTP). In the high-sulfate WWTPs, sulfate decreased in the anaerobic zone and increased in the oxic zone. Analysis of the sulfate reduction and sulfur oxidation activities of the sludge showed that both activities were dependent on the influent sulfate concentration. The relative abundance of SRB which oxidize organic matters completely in high-sulfate WWTPs tends to be higher than that in low-sulfate WWTPs. On the other hand, a lower relative abundance of polyphosphate accumulating organisms (PAOs) was recognized in the high-sulfate WWTPs.

show abstract

Microbial community change of sulfate reduction and sulfur oxidation bacteria in the activated sludge cultivated with acetate and peptone

Cited by 6 publications

References 16 publications

Short-sludge age EBPR process – Microbial and biochemical process characterisation during reactor start-up and operation

Short-sludge age EBPR process – Microbial and biochemical process characterisation during reactor start-up and operation

Efecto del sustrato como donante de electrones durante la reducción microbiana de sulfato y sus posibles aplicaciones en el tratamiento biológico de drenaje ácido de minas

Effects of Sulfates on Enhanced Biological Phosphorus Removal in Three Waste Water Treatment Plants

Contact Info

Product

Resources

About