Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail

Du, Xizhong; Xiang, Yun; Lou, Fangfang; Tu, Pingguang; Zhang, Xiaojun; Hu, Xujin; Lyu, Wentao; Xiao, Yingping

doi:10.3390/ani10061006

Cited by 29 publications

(19 citation statements)

References 41 publications

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“…It can reduce weight gain, hyperglycemia, and liver steatosis in ob/ob and high-fat diet mice [ 32 ] and significantly reduce the severity of intestinal inflammation in murine models of acute and chronic colitis [ 33 ]. The SCFA-producing Parabacteroides were richer in the cecum and colorectum, where, accordingly, more SCFAs were produced [ 34 ]. Lactococcus lactis activates innate immunity and protects from infections [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

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Maternal Vegetable and Fruit Consumption during Pregnancy and Its Effects on Infant Gut Microbiome

et al. 2021

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Maternal nutrition intake during pregnancy may affect the mother-to-child transmission of bacteria, resulting in gut microflora changes in the offspring, with long-term health consequences in later life. Longitudinal human studies are lacking, as only a small amount of studies showing the effect of nutrition intake during pregnancy on the gut microbiome of infants have been performed, and these studies have been mainly conducted on animals. This pilot study explores the effects of high or low fruit and vegetable gestational intake on the infant microbiome. We enrolled pregnant women with a complete 3-day dietary record and received postpartum follow-up. The 16S rRNA gene sequence was used to characterize the infant gut microbiome at 2 months (n = 39). Principal coordinate analysis ordination revealed that the infant gut microbiome clustered differently for high and low maternal fruit and vegetable consumption (p < 0.001). The linear discriminant analysis effect size and feature selection identified 6 and 17 taxa from both the high and low fruit and vegetable consumption groups. Among the 23 abundant taxa, we observed that six maternal intake nutrients were associated with nine taxa (e.g., Erysipelatoclostridium, Isobaculum, Lachnospiraceae, Betaproteobacteria, Burkholderiaceae, Sutterella, Clostridia, Clostridiales, and Lachnoclostridium). The amount of gestational fruit and vegetable consumption is associated with distinct changes in the infant gut microbiome at 2 months of age. Therefore, strategies involving increased fruit and vegetable consumption during pregnancy should be employed for modifying the gut microbiome early in life.

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Section: Discussionmentioning

confidence: 99%

“…Lactococcus lactis activates innate immunity and protects from infections [ 35 , 36 ]. Moreover, some Lactobacilli can produce SCFAs [ 34 ], which can induce Tregs to modulate gut immune responses [ 37 , 38 , 39 ], and can shape the pulmonary immune environment and influence the severity of allergic inflammation [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Maternal Vegetable and Fruit Consumption during Pregnancy and Its Effects on Infant Gut Microbiome

et al. 2021

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“…The genomic DNA was extracted from the jejunal and colonic contents of mice using QIAamp DNA Stool Mini Kit (QIAGEN, Valencia, CA, USA). The 16S rRNA gene sequencing and data analysis were performed as described previously (34). Briefly, the V3 and V4 region of bacterial 16S rRNA gene was amplified from each genomic DNA sample by using the barcode-fusion primers 341F and 806R.…”

Section: Dna Extraction and Microbiota Analysismentioning

confidence: 99%

“…The abundances of the major butyrate-producing bacteria, clostridial cluster I, IV, and XIVa and the terminal genes for butyrate synthesis, butyrate kinase (BK) and butyryl CoA: acetate CoA transferase (BCoAT) in mice colon contents were assessed by qPCR on an ABI Prism 7700 detection system (Applied Biosystems, Foster City, CA, USA) using the extracted DNA as templates and SYBR Green PCR Master Mix (Takara, Japan), as previously described (34,37…”

Section: Qpcr Analysis Of Key Bacteria and Genes In Butyrate Productionmentioning

confidence: 99%

Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs

Lyu

Hong

et al. 2021

Front. Nutr.

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Gut microbiota is recognized as a strong determinant of host physiology including fat metabolism and can transfer obesity-associated phenotypes from donors to recipients. However, the relationship between gut microbiota and intramuscular fat (IMF) is still largely unknown. Obese Jinhua pigs (JP) have better meat quality that is associated with higher IMF content than lean Landrace pigs (LP). The present study was conducted to test the contribution of gut microbiota to IMF properties by transplanting fecal microbiota of adult JP and LP to antibiotics-treated mice. Similar to JP donors, the mice receiving JP's microbiota (JM) had elevated lipid and triglyceride levels and the lipoprotein lipase activity, as well as reduced mRNA level of angiopoietin-like 4 (ANGPTL4) in the gastrocnemius muscles, compared to those in mice receiving LP's microbiota (LM). High-throughput 16S rRNA sequencing confirmed that transplantation of JP and LP feces differently reconstructed the gut microbiota in both jejunum and colon of mouse recipients. In colonic samples, we observed an elevated ratio of Firmicutes to Bacteroidetes and increased abundance of genus Romboutsia in JM, which were positively correlated with obesity. Furthermore, the abundance of Akkermansia decreased in JM, which is positively correlated with lean. Colonic concentrations of acetate (P = 0.047) and butyrate (P = 0.014) were significantly lower in JM than in LM, and consistently, the terminal genes for butyrate synthesis, butyryl CoA: acetate CoA transferase were less abundant in colonic microbiota of JM. Taken together, these gut microbiota of obese JP intrinsically promotes IMF accumulation and can transfer the properties to mouse recipients. Manipulation of intestinal microbiota will, therefore, have the potential to improve the meat quality and flavor of pigs and even to ameliorate the metabolic syndrome in human.

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“…It has been shown that Lactobacillus culture possess beneficial effects on immune response and cytokines in chickens [17,19]; moreover, it can limit Salmonella colonization [20,21]. Although Lactobacillus is a predominant genus of bacteria normally inhabitant in poultry intestines [22,23]; however, to the best of our knowledge, there is little information available on chicken immune responses to probiotic bacteria, especially when LA is used as a probiotic. Therefore, this study aimed to evaluate the impact of dietary probiotic LA supplementation on cholesterol levels, immune response, and productive performance of laying hens.…”

Section: Introductionmentioning

confidence: 99%

Dietary Supplementation of Probiotic Lactobacillus acidophilus Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens

Alaqil

Abbas

El-Beltagi

et al. 2020

Animals

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This study examines the effect of dietary supplementation with Lactobacillus acidophilus (LA) on the cholesterol levels, immune response, and productive performance of laying hens. A total of 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into four treatment groups (18 birds × three replicates per group) and fed diet supplemented with 0 (control), 1 × 109, 21 × 109, and 31 × 109 colony forming units (CFUs) of Lactobacillus acidophilus (LA) per kg of feed for six consecutive weeks. Results show that plasma triglycerides, low-density lipoprotein (LDL) and total cholesterols became lesser, while high-density lipoprotein (HDL) cholesterol became higher in LA-supplemented groups compared to the control. In addition, a significant reduction occurred in the liver and egg yolk cholesterol by LA supplementation. Moreover, the immunological parameters including antibody titer against sheep red blood cells (SRBCs), phytohemagglutinin (PHA)-wattle swelling test, and T- & B-lymphocyte proliferation were enhanced in laying hens supplemented with LA compared to the control hens. While the heterophil to lymphocyte (H/L) ratio decreased with LA supplementation, indicating low stress conditions in the treated hens. These positive effects for LA were further reflected on the productive performance of laying hens and improved egg production, egg weight, egg mass, and feed efficiency. Our findings indicate that LA probiotic could be recommended in laying hens’ diets for lowering egg yolk cholesterol with positive impacts on health and performance.

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Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail

Cited by 29 publications

References 41 publications

Maternal Vegetable and Fruit Consumption during Pregnancy and Its Effects on Infant Gut Microbiome

Maternal Vegetable and Fruit Consumption during Pregnancy and Its Effects on Infant Gut Microbiome

Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs

Dietary Supplementation of Probiotic Lactobacillus acidophilus Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens

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