Microbial Community and in situ Bioremediation of Groundwater by Nitrate Removal in the Zone of a Radioactive Waste Surface Repository

Safonov, Alexey; Бабич, Т. Л.; Соколова, Д. Ш.; Grouzdev, Denis S.; Tourova, T. P.; Полтараус, А. Б.; Zakharova, Еlena V.; Merkel, Alexander Y.; Новиков, А. П.; Назина, Т. Н.

doi:10.3389/fmicb.2018.01985

Cited by 55 publications

(35 citation statements)

References 67 publications

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“…The use of whey may also be an economically feasible solution to attenuate NO3pollution, while providing waste recycling. To the authors' knowledge, although a few previous studies focused on N removal by lactic acid derived products (Fernández-Nava et al, 2010;Safonov et al, 2018;Sage et al, 2006;Tang et al, 2018), an assessment of whey recycling to promote denitrification by means of isotopic tools has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the potential use of a dairy industry residue to induce denitrification in polluted water bodies: A flow-through experiment

Margalef-Marti

Carrey

Soler

et al. 2019

Journal of Environmental Management

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Section: Introductionmentioning

confidence: 99%

Evaluating the potential use of a dairy industry residue to induce denitrification in polluted water bodies: A flow-through experiment

Margalef-Marti

Carrey

Soler

et al. 2019

Journal of Environmental Management

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“…Soil phosphatase was determined with the disodium phenyl phosphate method ( Shao et al, 2020 ). Soil nitrite reductase was first leached with sodium nitrite-aluminum potassium alum, and then measured through a colorimetric method ( Safonov et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Microbial Responses to the Reduction of Chemical Fertilizers in the Rhizosphere Soil of Flue-Cured Tobacco

Shen

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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The overuse of chemical fertilizers has resulted in the degradation of the physicochemical properties and negative changes in the microbial profiles of agricultural soil. These changes have disequilibrated the balance in agricultural ecology, which has resulted in overloaded land with low fertility and planting obstacles. To protect the agricultural soil from the effects of unsustainable fertilization strategies, experiments of the reduction of nitrogen fertilization at 10, 20, and 30% were implemented. In this study, the bacterial responses to the reduction of nitrogen fertilizer were investigated. The bacterial communities of the fertilizer-reducing treatments (D10F, D20F, and D30F) were different from those of the control group (CK). The alpha diversity was significantly increased in D20F compared to that of the CK. The analysis of beta diversity revealed variation of the bacterial communities between fertilizer-reducing treatments and CK, when the clusters of D10F, D20F, and D30F were separated. Chemical fertilizers played dominant roles in changing the bacterial community of D20F. Meanwhile, pH, soil organic matter, and six enzymes (soil sucrase, catalase, polyphenol oxidase, urease, acid phosphatase, and nitrite reductase) were responsible for the variation of the bacterial communities in fertilizer-reducing treatments. Moreover, four of the top 20 genera (unidentified JG30-KF-AS9, JG30-KF-CM45, Streptomyces, and Elsterales) were considered as key bacteria, which contributed to the variation of bacterial communities between fertilizer-reducing treatments and CK. These findings provide a theoretical basis for a fertilizer-reducing strategy in sustainable agriculture, and potentially contribute to the utilization of agricultural resources through screening plant beneficial bacteria from native low-fertility soil.

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“…Several of these genera include nitrate-reducing or denitrifying members, many of which have been identified previously in 16S rRNA gene studies of nitrate- and uranium-contaminated groundwater from the Oak Ridge Reservation, including Hydrogenophaga , Acinetobacter , Mesorhizobium , and Sphingobium [ 47 ]. Nitrate and concomitant contaminants have been significantly linked to the presence of members of Oxalobacteraceae and Acinetobacter in nitrate and uranium contaminated groundwater in Russia [ 48 ] and Massilia with nitrate concentration in previous in-field bioreactor experiments [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

In-field bioreactors demonstrate dynamic shifts in microbial communities in response to geochemical perturbations

et al. 2020

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Subsurface microbial communities mediate the transformation and fate of redox sensitive materials including organic matter, metals and radionuclides. Few studies have explored how changing geochemical conditions influence the composition of groundwater microbial communities over time. We temporally monitored alterations in abiotic forces on microbial community structure using 1L in-field bioreactors receiving background and contaminated groundwater at the Oak Ridge Reservation, TN. Planktonic and biofilm microbial communities were initialized with background water for 4 days to establish communities in triplicate control reactors and triplicate test reactors and then fed filtered water for 14 days. On day 18, three reactors were switched to receive filtered groundwater from a contaminated well, enriched in total dissolved solids relative to the background site, particularly chloride, nitrate, uranium, and sulfate. Biological and geochemical data were collected throughout the experiment, including planktonic and biofilm DNA for 16S rRNA amplicon sequencing, cell counts, total protein, anions, cations, trace metals, organic acids, bicarbonate, pH, Eh, DO, and conductivity. We observed significant shifts in both planktonic and biofilm microbial communities receiving contaminated water. This included a loss of rare taxa, especially amongst members of the Bacteroidetes, Acidobacteria, Chloroflexi, and Betaproteobacteria, but enrichment in the Fe-and nitrate-reducing Ferribacterium and parasitic Bdellovibrio. These shifted communities were more similar to the contaminated well community, suggesting that geochemical forces substantially influence microbial community diversity and structure. These influences can only be captured through such comprehensive temporal studies, which also enable more robust and accurate predictive models to be developed.

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Microbial Community and in situ Bioremediation of Groundwater by Nitrate Removal in the Zone of a Radioactive Waste Surface Repository

Cited by 55 publications

References 67 publications

Evaluating the potential use of a dairy industry residue to induce denitrification in polluted water bodies: A flow-through experiment

Evaluating the potential use of a dairy industry residue to induce denitrification in polluted water bodies: A flow-through experiment

Microbial Responses to the Reduction of Chemical Fertilizers in the Rhizosphere Soil of Flue-Cured Tobacco

In-field bioreactors demonstrate dynamic shifts in microbial communities in response to geochemical perturbations

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