Microbial community analysis of mesophilic anaerobic protein degradation process using bovine serum albumin (BSA)-fed continuous cultivation

Tang, Yue‐Qin; Shigematsu, Toru; Morimura, Shigeru; Kida, Kenji

doi:10.1263/jbb.99.150

Cited by 126 publications

(75 citation statements)

References 44 publications

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“…They are able to ferment proteins or amino acids to produce VFAs, H 2 , and CO 2 (Ganesan et al 2008;Looft et al 2013). It was found that Aminobacterium played a dominant role in a methanogenic digester containing bovine serum albumin as a sole carbon source (Tang et al 2005). Though Aminobacterium was not significantly correlated with the dominant hydrogenotrophic methanogens in this study, its stable and high relative abundance throughout the fermentation process implicated its constitutive role in the degradation of amino acids.…”

Section: Discussionmentioning

confidence: 52%

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Rui

Pei

et al. 2014

Appl Microbiol Biotechnol

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Anaerobic co-fermentation of straw and manure is widely used for waste treatment and biogas production. However, the differences between the straw-and slurry-associated prokaryotic communities, their dynamic changes throughout the co-fermentation process, and their correlations with bioreactor performance are not fully understood. To address these questions, we investigated the prokaryotic community compositions and the dynamics of prokaryotes attached to the straw and in the slurry during co-fermentation of wheat straw and swine manure using pyrosequencing technique. The results showed that straw-and slurry-associated prokaryotes were different in their structure and function. Strawassociated prokaryotic communities were overrepresented by the phyla Spirochaetes and Fibrobacteres, while Synergistetes and Euryarchaeota were more abundant in the slurry. The straw-associated candidate class TG3, genera Fibrobacter, Bacteroides, Acetivibrio, Clostridium III, Papillibacter, Treponema, Sedimentibacter, and Lutispora may specialize in substrate hydrolysis. Propionate was the most abundant volatile fatty acid in the slurry, and it was probably degraded through syntrophic oxidation by the genera Pelotomaculum, Methanoculleus, and Methanosaeta. The protein-fermenting bacteria Aminobacterium and Cloacibacillus were much abundant in the slurry, indicating that proteins are important substrates in the co-fermentation. This study provided a better understanding of the anaerobic co-fermentation process that is driven by spatially differentiated microbiota.

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Section: Discussionmentioning

confidence: 52%

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Rui

Pei

et al. 2014

Appl Microbiol Biotechnol

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“…Firmicutes and Bacteroidetes communities were very stable during the digestion of the substrates in biogas reactors fed with casein, starch, and cream and only starving periods, changes of pH values, or long-time continuous feeding caused species shifts in bacterial community (Kampmann et al 2012). A predominance of Bacteroidetes (even over 58% of all bacteria) was noted in reactors treating protein-rich substrates such as casein or bovine serum albumin (Tang et al 2005, Kampmann et al 2012). In the investigated sewage-sludge fed digesters they probably played an important role in degradation of protein and amino acids to acetate and ammonia.…”

Section: Discussionmentioning

confidence: 99%

Microbiota of anaerobic digesters in a full-scale wastewater treatment plant

Świątczak

Cydzik‐Kwiatkowska

Rusanowska

2017

Archives of Environmental Protection

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Abstract:Anaerobic digestion is an important technology for the bio-based economy. The stability of the process is crucial for its successful implementation and depends on the structure and functional stability of the microbial community. In this study, the total microbial community was analyzed during mesophilic fermentation of sewage sludge in full-scale digesters.The digesters operated at 34-35°C, and a mixture of primary and excess sludge at a ratio of 2:1 was added to the digesters at 550 m 3 /d, for a sludge load of 0.054 m 3 /(m 3 ·d). The amount and composition of biogas were determined. The microbial structure of the biomass from the digesters was investigated with use of next-generation sequencing.The percentage of methanogens in the biomass reached 21%, resulting in high quality biogas (over 61% methane content). The abundance of syntrophic bacteria was 4.47%, and stable methane production occurred at a Methanomicrobia to Synergistia ratio of 4.6:1.0. The two most numerous genera of methanogens (about 11% total) were Methanosaeta and Methanolinea, indicating that, at the low substrate loading in the digester, the acetoclastic and hydrogenotrophic paths of methane production were equally important. The high abundance of the order Bacteroidetes, including the class Cytophagia (11.6% of all sequences), indicated the high potential of the biomass for effi cient degradation of lignocellulitic substances, and for degradation of protein and amino acids to acetate and ammonia.This study sheds light on the ecology of microbial groups that are involved in mesophilic fermentation in mature, stably-performing microbiota in full-scale reactors fed with sewage sludge under low substrate loading.

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“…In addition, VadinBC27_wastewater-sludge_group was also observed throughout the operation period. It might be participating in the degradation of amino acids and some refractory organic matters [56][57][58]. The genus Smithella, which could degrade propionate to acetate [42,59], was dominant in start-up (3.13%) and winter (3.5%), and this was probably one of the reasons of acetate accumulation.…”

Section: Shifts In Microbial Community Structures With Seasonal Tempementioning

confidence: 99%

Effect of Seasonal Temperature on the Performance and on the Microbial Community of a Novel AWFR for Decentralized Domestic Wastewater Pretreatment

Lü

2017

Applied Sciences

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Due to environmental burden and human health risks in developing countries, the treatment of decentralized domestic wastewater has been a matter of great concern in recent years. A novel pilot-scale three-stage anaerobic wool-felt filter reactor (AWFR) was designed to treat real decentralized domestic wastewater at seasonal temperature variations of 8 to 35 • C for 364 days. The results showed that the average chemical oxygen demand (COD) removal efficiencies of AWFR in summer and winter were 76 ± 7.2% and 52 ± 5.9% at one day and three days Hydraulic Retention Time (HRT), respectively. COD mass balance analysis demonstrated that even though COD removal was lower in winter, approximately 43.5% of influent COD was still converted to methane. High-throughput MiSeq sequencing analyses indicated that Methanosaeta, Methanobacterium, and Methanolinea were the predominant methanogens, whereas the genus Bacillus probably played important roles in fermentation processes throughout the whole operation period. The performance and microbial community composition study suggested the application potential of the AWFR system for the pretreatment of decentralized domestic wastewater.

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Microbial community analysis of mesophilic anaerobic protein degradation process using bovine serum albumin (BSA)-fed continuous cultivation

Cited by 126 publications

References 44 publications

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure

Microbiota of anaerobic digesters in a full-scale wastewater treatment plant

Effect of Seasonal Temperature on the Performance and on the Microbial Community of a Novel AWFR for Decentralized Domestic Wastewater Pretreatment

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