Microbial communities involved in aerobic and anaerobic methane cycling in a meromictic ferruginous subarctic lake

Kallistova, A. Yu.; Kadnikov, Vitaly V.; Rusanov, Igor I; Kokryatskaya, N. M.; Beletsky, Alexey V.; Mardanov, Andrey V.; Savvichev, Alexander S; Ravin, Nikolai V.; Пименов, Н. В.

doi:10.3354/ame01878

Cited by 17 publications

(35 citation statements)

References 86 publications

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“…Aerobic methanotrophic members of Alpha-(type II) and Gammaproteobacteria (type I) are typical inhabitants of freshwater lakes, and gammaproteobacterial Methylobacter spp. are the most frequently detected methanotrophs in various freshwater habitats, including boreal lakes [37,38]. Members of the Methylococcaceae family were also shown to be predominant in some thermokarst lakes of Alaska [29,39] and Canada [40].…”

Section: Discussionmentioning

confidence: 99%

Microbiological Study of Yamal Lakes: A Key to Understanding the Evolution of Gas Emission Craters

et al. 2018

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Although gas emission craters (GECs) are actively investigated, the question of which landforms result from GECs remains open. The evolution of GECs includes the filling of deep hollows with atmospheric precipitation and deposits from their retreating walls, so that the final stage of gas emission crater (GEC) lake development does not differ from that of any other lakes. Microbial activity and diversity may be indicators that make it possible to distinguish GEC lakes from other exogenous lakes. This work aimed at a comparison of the activity and diversity of microbial communities in young GEC lakes and mature background lakes of Central Yamal by using a radiotracer analysis and high-throughput sequencing of the 16S rRNA genes. The radiotracer analysis revealed slow-flowing microbial processes as expected for the cold climate of the study area. GEC lakes differed from background ones by slow rates of anaerobic processes (methanogenesis, sulfate reduction) as well as by a low abundance and diversity of methanogens. Other methane cycle micro-organisms (aerobic and anaerobic methanotrophs) were similar in all studied lakes and represented by Methylobacter and ANME 2d; the rates of methane oxidation were also similar. Actinobacteria, Bacteroidetes, Betaproteobacteria, and Acidobacteria were predominant in both lake types. Thus, GEC lakes may be identified by their scarce methanogenic population.

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Section: Discussionmentioning

confidence: 99%

Microbiological Study of Yamal Lakes: A Key to Understanding the Evolution of Gas Emission Craters

et al. 2018

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“…The role of boreal lakes in worldwide greenhouse gas emissions is receiving increasing attention. Earlier studies mainly highlighted the large role of aerobic CH 4 oxidation in the lake carbon cycle (Kankaala et al, 2006). More recent studies have shown that boreal lakes can exhibit highly active CH 4 -oxidizing communities both in the oxic and anoxic parts of the water column (Taipale et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Methane oxidation in the waters of a humic-rich boreal lake stimulated by photosynthesis, nitrite, Fe(III) and humics

et al. 2021

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Abstract. Small boreal lakes are known to contribute significantly to global CH4 emissions. Lake Lovojärvi is a eutrophic lake in southern Finland with bottom water CH4 concentrations up to 2 mM. However, the surface water concentration, and thus the diffusive emission potential, was low (< 0.5 µM). We studied the biogeochemical processes involved in CH4 removal by chemical profiling and through incubation experiments. δ13C-CH4 profiling of the water column revealed a methane-oxidation hotspot just below the oxycline and zones of CH4 oxidation within the anoxic water column. In incubation experiments involving the addition of light and/or oxygen, CH4 oxidation rates in the anoxic hypolimnion were enhanced 3-fold, suggesting a major role for photosynthetically fueled aerobic CH4 oxidation. We observed a distinct peak in CH4 concentration at the chlorophyll-a maximum, caused by either in situ CH4 production or other CH4 inputs such as lateral transport from the littoral zone. In the dark anoxic water column at 7 m depth, nitrite seemed to be the key electron acceptor involved in CH4 oxidation, yet additions of Fe(III), anthraquinone-2,6-disulfonate and humic substances also stimulated anoxic CH4 oxidation. Surprisingly, nitrite seemed to inhibit CH4 oxidation at all other depths. Overall, this study shows that photosynthetically fueled CH4 oxidation can be a key process in CH4 removal in the water column of humic, turbid lakes, thereby limiting diffusive CH4 emissions from boreal lakes. Yet, it also highlights the potential importance of a whole suite of alternative electron acceptors, including humics, in these freshwater environments in the absence of light and oxygen.

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“…However, the identity of EAs driving AOM in lake sediments is understudied and such data is especially scarce from boreal lakes (Rissanen et al 2017; Kallistova et al 2018; Martinez-Cruz et al 2018). In the few previous studies, additions of Fe 3+ , Mn 4+ , SO 4 2- or NO 3 - did not enhance AOM in sediments of boreal lakes (Rissanen et al 2017; Kallistova et al 2018). This was despite the fact that the sediments harbored typical freshwater AOM organisms, i.e.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic oxidation of methane in sediments of a nitrate-rich, oligo-mesotrophic boreal lake

Rissanen

Jilbert

Simojoki

et al. 2021

Preprint

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The importance of nitrate in promoting anaerobic oxidation of methane (AOM) in the sediments of boreal lakes is currently unknown. Here we investigated the extent to which sediment AOM is linked to nitrate reduction in a nitrate-rich, oligo-mesotrophic, boreal lake (Lake Paajarvi, Finland). AOM potential of sediment slurries, collected from three profundal stations of the study lake, was measured at varying nitrate concentrations using 13C-labelling. This was coupled with analysis of vertical profiles of the sediment and porewater geochemistry and the microbial communities (16S rRNA gene and shotgun metagenomic sequencing). Sediment AOM potential was enhanced by nitrate at a shallow station with high contents of labile phytoplankton-derived organic matter (Station 1), but not at the other two stations. AOM was also much higher at Station 1 (2.0-6.8 nmol C cm-3 d-1) than at the other stations (0-0.3 nmol C cm-3 d-1). Accordingly, methanotrophic archaea (Candidatus Methanoperedens sp.) and bacteria (Methylococcales) had the highest relative abundance at Station 1. Furthermore, geochemical profiles indicated that AOM was potentially coupled with reduction of nitrate, iron, or sulfate at all stations. We conclude that AOM linked to nitrate reduction takes place in boreal lake sediments. However, our data cannot resolve whether nitrate affects the process directly, via enhancing nitrate reduction-mediated AOM, or indirectly, via enhancing AOM mediated by reduction of other electron acceptors.

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Microbial communities involved in aerobic and anaerobic methane cycling in a meromictic ferruginous subarctic lake

Cited by 17 publications

References 86 publications

Microbiological Study of Yamal Lakes: A Key to Understanding the Evolution of Gas Emission Craters

Microbiological Study of Yamal Lakes: A Key to Understanding the Evolution of Gas Emission Craters

Methane oxidation in the waters of a humic-rich boreal lake stimulated by photosynthesis, nitrite, Fe(III) and humics

Anaerobic oxidation of methane in sediments of a nitrate-rich, oligo-mesotrophic boreal lake

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