Microbial Communities in Soils and Endosphere of Solanum tuberosum L. and their Response to Long-Term Fertilization

Kracmarova, Martina; Karpíšková, Jana; Uhlík, Ondřej; Strejcek, Michal; Száková, Jiřina; Balík, J.; Demnerova, Kateřina; Stiborová, Hana

doi:10.3390/microorganisms8091377

Cited by 20 publications

(11 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study is a follow up to our previous work in which we described the response of endophytic microbial communities to the long-term fertilization of agricultural soils [29]. Since the community structure was significantly altered, in this study we focused on the metabolic processes happening in some of these fertilized soils.…”

Section: Introductionmentioning

confidence: 96%

Response of Soil Microbes and Soil Enzymatic Activity to 20 Years of Fertilization

et al. 2020

Self Cite

View full text Add to dashboard Cite

Fertilization is a worldwide agricultural practice used in agronomy to increase crop yields. Fertilizer application influences overall soil characteristics, including soil microbial community composition and metabolic processes mediated by microbial enzymatic activity. Changes in the structure of microbial communities and their metabolic activity after long-term fertilization were studied in this research. We hypothesized that the different types of fertilization regimes affect nutrient levels in the soil which subsequently influence the metabolic processes and microbial diversity and community structure. Manure (MF; 330 kg N/ha), sewage sludge at two application doses (SF; 330 kg N/ha and SF3x; 990 kg N/ha) and chemical (NPK; N-P-K nutrients in concentrations of 330-90-300 kg/ha) fertilizers have been applied regularly to an experimental field since 1996. The microbial diversity increased in all soils amended with both organic (MF, SF, SF3x) and chemical (NPK) fertilizers. The shifts in microbial communities were observed, which were mainly caused by less abundant genera that were mostly associated with one or more fertilization treatment(s). Fertilization also influenced soil chemistry and the activity of β-xylosidase, β-N-acetylglucosaminidase (NAG), acid phosphatase and FDA-hydrolases. Specifically, all fertilization treatments were associated with a higher activity of β xylosidase and lower NAG activity. Only the NPK treatment was associated with a higher activity of acid phosphatase.

show abstract

Section: Introductionmentioning

confidence: 96%

Response of Soil Microbes and Soil Enzymatic Activity to 20 Years of Fertilization

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The DNA library for the ITS region was prepared by using specific primers and two-step PCR. The forward primer 5.8S_Fun 5-AACTTTYRRCAAYGGATCWCT-3 and reverse primer ITS4_Fun 5-AGCCTCCGCTTATTGATATGCTTAART-3 [17] were used for amplification of the ITS-2 region (all Sigma-Aldrich, St. Louis, MO, USA). The master mix content and temperature programs were adopted from a study by Kracmarova et al [17] with a few modifications.…”

Section: Dna Library Preparing and Sequencingmentioning

confidence: 99%

“…The forward primer 5.8S_Fun 5-AACTTTYRRCAAYGGATCWCT-3 and reverse primer ITS4_Fun 5-AGCCTCCGCTTATTGATATGCTTAART-3 [17] were used for amplification of the ITS-2 region (all Sigma-Aldrich, St. Louis, MO, USA). The master mix content and temperature programs were adopted from a study by Kracmarova et al [17] with a few modifications. Briefly, the first PCR reaction mix contained nuclease-free water, 1 µM of each of the appropriate primers, 0.02 U/µL of KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, MA, USA), and 2 µL of template in a total reaction volume of 15 µL.…”

Section: Dna Library Preparing and Sequencingmentioning

confidence: 99%

Factors Influencing the Fungal Diversity on Audio–Visual Materials

et al. 2021

Self Cite

View full text Add to dashboard Cite

The biodeterioration of audio–visual materials is a huge problem, as it can cause incalculable losses. To preserve these cultural heritage objects for future generations, it is necessary to determine the main agents of biodeterioration. This study focuses on identifying fungi, both from the air and smears from photographs and cinematographic films that differ in the type of carrier and binder, using high-throughput sequencing approaches. The alpha diversity measures of communities present on all types of carriers were compared, and a significant difference between cellulose acetate and baryta paper was observed. Next, the locality, type of carrier, and audio–visual material seem to affect the structure of fungal communities. Additionally, a link between the occurrence of the most abundant classes and species on audio–visual materials and air contamination in the archives was proven. In both cases, the most abundant classes were Agariomycetes, Dothideomycetes, and Eurotiomycetes, and approximately half of the 50 most abundant species detected on the audio–visual materials and in the air were identical.

show abstract

“…Our previous studies have shown how long-term fertilization influences soil enzymatic activity [ 38 ] and endophytic communities in potatoes [ 39 ]. This study focused on the effects of long-term crop rotations, and organic and chemical fertilizer application on soil bacterial and fungal communities in various soil types.…”

Section: Introductionmentioning

confidence: 99%

Soil microbial communities following 20 years of fertilization and crop rotation practices in the Czech Republic

Kracmarova

Uhlík

Strejcek

et al. 2022

Environmental Microbiome

Self Cite

View full text Add to dashboard Cite

Background Although fertilization and crop rotation practices are commonly used worldwide in agriculture to maximize crop yields, their long-term effect on the structures of soil microorganisms is still poorly understood. This study investigated the long-term impact of fertilization and crop rotation on soil microbial diversity and the microbial community structure in four different locations with three soil types. Since 1996, manure (MF; 330 kg N/ha), sewage sludge (SF; 330 and SF3x; 990 kg N/ha), and NPK (NPK; 330 kg N/ha) fertilizers were periodically applied to the soils classified as chernozem, luvisol and cambisol, which are among the most abundant or fertile soils used for agricultural purposes in the world. In these soils, potato (Solanum tuberosum L.), winter wheat (Triticum aestivum L.), and spring barley (Hordeum vulgare L.) were rotated every three years. Results Soil chemistry, which was significantly associated with location, fertilization, crop rotation, and the interaction of fertilization and location, was the dominant driver of soil microbial communities, both prokaryotic and fungal. A direct effect of long-term crop rotation and fertilization on the structure of their communities was confirmed, although there was no evidence of their influence on microbial diversity. Fungal and bacterial communities responded differently to fertilization treatments; prokaryotic communities were only significantly different from the control soil (CF) in soils treated with MF and SF3x, while fungal communities differed across all treatments. Indicator genera were identified for different treatments. These taxa were either specific for their decomposition activities or fungal plant pathogens. Sequential rotation of the three crops restricted the growth of several of the indicator plant pathogens. Conclusions Long-term fertilization and crop rotation significantly altered microbial community structure in the soil. While fertilization affected soil microorganisms mainly through changes in nutrient profile, crop rotations lead to the attraction and repulsion of specific plant pathogens. Such changes in soil microbial communities need to be considered when planning soil management.

show abstract

Microbial Communities in Soils and Endosphere of Solanum tuberosum L. and their Response to Long-Term Fertilization

Cited by 20 publications

References 84 publications

Response of Soil Microbes and Soil Enzymatic Activity to 20 Years of Fertilization

Response of Soil Microbes and Soil Enzymatic Activity to 20 Years of Fertilization

Factors Influencing the Fungal Diversity on Audio–Visual Materials

Soil microbial communities following 20 years of fertilization and crop rotation practices in the Czech Republic

Contact Info

Product

Resources

About