Microbial communities associated with the ostracods Candona sp. inhabiting the area of the methane seep Goloustnoye (Lake Baikal)

Хальзов, И. А.; Bukin, S. V.; Zakharenko, А. S.; Chernitsyna, S. M.; Galachyants, Yuri P.; Sitnikova, T. Ya.; Zemskaya, Т. I.

doi:10.1007/s13199-021-00802-3

Cited by 7 publications

(2 citation statements)

References 64 publications

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“…Movile Cave and surrounding wells in the Mangalia region, highlighting the versatility of Thiothrix-crustacean associations in sulfidic ecosystems. This is the first report of an association between Thiothrix and groundwater microcrustaceans, and the second report of an association between Thiothrix and a nonmarine ostracod (Khalzov et al 2021).…”

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confidence: 67%

Identification of sulfur–oxidizing Thiothrix bacteria on microcrustaceans from the sulfidic groundwaters of Mangalia (southeastern Romania)

Filip¹,

Chauveau²,

Flot³

et al. 2022

ACA

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Movile Cave, located in southeastern Romania close to the Black Sea, is one of the most remarkable diversity hotspots worldwid with 52 species of invertebrates of which 37 are endemic (Brad et al. 2021). Due to the high concentration of hydrogen sulfide in its water, the primary production of organic matter in Movile Cave relies almost entirely on the chemoautotrophic activity of microorganisms, notably sulfur-oxidizing gammaproteobacteria belonging to the genus Thiothrix. In the presence of oxygen, these filamentous bacteria can oxidize hydrogen sulfide and reduce it into various sulfidic compounds, generating energy in the process. In sulfidic ecosystems, Thiothrix bacteria are frequently found free-living but also as epibionts or ectosymbionts growing on other organisms, such as amphipods (Flot et al. 2014). However, it is unclear whether Thiothrix bacteria also grow on microcrustaceans such as copepods or ostracods, of which several species are known from the sulfidic mesothermal aquifer of Mangalia, where Movile Cave is located. To find it out, we combined DNA sequencing using the reference bacterial 16S ribosomal RNA gene with morphological observations (including fluorescence microscopy). Our results reveal that Thiothrix bacteria are indeed present on microcrustaceans from Movile Cave and surrounding wells in the Mangalia region, highlighting the versatility of Thiothrix-crustacean associations in sulfidic ecosystems. This is the first report of an association between Thiothrix and groundwater microcrustaceans, and the second report of an association between Thiothrix and a nonmarine ostracod (Khalzov et al. 2021).

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mentioning

confidence: 67%

Identification of sulfur–oxidizing Thiothrix bacteria on microcrustaceans from the sulfidic groundwaters of Mangalia (southeastern Romania)

Filip¹,

Chauveau²,

Flot³

et al. 2022

ACA

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“…Among the dominant taxa of B. calyciflorus -associated bacteria, unclassified Alphaproteobacteria and A llorhizobium-Neorhizobium-Pararhizobium-Rhizobium of Rhizobiales were the two genera with the highest relative abundance, which have no obvious changing trend during the whole population growth cycle. Studies have shown that Alphaproteobacteria is not only dominant in rotifer-associated bacteria, but also widely exists in Cladocera ( Grossart et al, 2009 ), Copepods ( Dziallas et al, 2013 ) and Protozoa ( Khalzov et al, 2021 ). The members of Rhizobiales are mostly nitrogen-fixing bacteria, which are the most common plant symbioses and also play a potential role in the carbon cycle of freshwater lakes ( Erlacher et al, 2015 ; Wang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Population dynamics of Brachionus calyciflorus driven by the associated natural bacterioplankton

et al. 2023

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Zooplankton provides bacteria with a complex microhabitat richen in organic and inorganic nutrients, and the bacteria community also changes the physiochemical conditions for zooplankton, where the symbiotic relationship between them plays an important role in the nutrient cycle. However, there are few studies on the effect of associated bacteria on the population dynamics of rotifers. In order to make clear their relationships, we reconstructed the associated bacterial community in Brachionus calyciflorus culture, and examined the life history and population growth parameters, and analyzed the diversity and community composition of the associated bacteria at different growth stages of B. calyciflorus. The results showed that the addition of bacteria from natural water can promote the population growth and asexual reproduction of B. calyciflorus, but has no significant effect on sexual reproduction, exhibited by the improvement of its life expectancy at hatching, net reproduction rates and intrinsic growth rate, no significant effects on the generation time and mixis ratio of offspring. It was found that the B. calyciflorus-associated bacterial community was mainly composed of Proteobacteria, Bacteroidota, Actinobacteriota, Cyanobacteria and Firmicutes. Through correlation network analysis, the members of Burkholderiales, Pseudomonadales, Micrococcales, Caulobacterales and Bifidobacteriales were the keystone taxa of B. calyciflorus-associated bacteria. In addition, the relative abundance of some specific bacteria strains increased as the population density of B. calyciflorus increased, such as Hydrogenophaga, Acidovorax, Flavobacterium, Rheinheimera, Novosphingobium and Limnobacter, and their relative abundance increased obviously during the slow and exponential phases of population growth. Meanwhile, the relative abundance of adverse taxa (such as Elizabethkingia and Rickettsiales) decreased significantly with the increase in rotifer population density. In conclusion, the closely associated bacteria are not sufficient for the best growth of B. calyciflorus, and external bacterioplankton is necessary. Furthermore, the function of keystone and rare taxa is necessary for further exploration. The investigation of the symbiotic relationship between zooplankton-associated bacterial and bacterioplankton communities will contribute to monitoring their roles in freshwater ecosystems, and regulate the population dynamics of the micro-food web.

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Genomic and morphological characterization of a new Thiothrix species from a sulfide hot spring of the Zmeinaya bay (Northern Baikal, Russia)

Chernitsyna,

Elovskaya,

Bukin

et al. 2024

Antonie van Leeuwenhoek

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Microbial communities associated with the ostracods Candona sp. inhabiting the area of the methane seep Goloustnoye (Lake Baikal)

Cited by 7 publications

References 64 publications

Identification of sulfur–oxidizing Thiothrix bacteria on microcrustaceans from the sulfidic groundwaters of Mangalia (southeastern Romania)

Identification of sulfur–oxidizing Thiothrix bacteria on microcrustaceans from the sulfidic groundwaters of Mangalia (southeastern Romania)

Population dynamics of Brachionus calyciflorus driven by the associated natural bacterioplankton

Genomic and morphological characterization of a new Thiothrix species from a sulfide hot spring of the Zmeinaya bay (Northern Baikal, Russia)

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