Microbial cleavage of zearalenone

Abstract

1. Zearalenone, a fungal oestrogenic compound, was subjected to microbial transformation studies. Preliminary screening with 150 fungal species showed that Gliocladium roseum was capable of metabolizing zearalenone in 80-90% yields. 2. Large-scale fermentation with G. roseum produced a 1:1 mixture of 1-(3,5-dihydroxyphenyl)-10'-hydroxy-1-undecen-6'-one and 1-(3,5-dihydroxyphenyl)-6'-hydroxy-1-undecen-10'-one. The compounds were isolated and purified at -20 degrees C, and identified using spectroscopic analysis and by comparison to products obtained from alkaline hydrolysis of zearalenone.

Detoxification of Zearalenone by viable and inactivated cells of Planococcus sp

“…A number of studies on the degradation and biotransformation of ZEN by various microorganisms have been published. Significant biodegradation of ZEN has been observed by the mycoparasite Gliocladium roseum NRRL 1859, which is capable of metabolizing ZEN in 80%e90% yields (El-Sharkawy & Abul-Hajj, 1988). The strain could split the lactone ring of ZEN, and the product, which is far less oestrogenic than ZEN, consisting of a mixture of two isomeric hydroxyketones, decarboxylated spontaneously, rendering the reaction irreversible.…”

Localization of zearalenone detoxification gene(s) in pZEA-1 plasmid of Pseudomonas putida ZEA-1 and expressed in Escherichia coli

“…These include adsorption [9,10], biodegradation [11][12][13] and ozonation [14]. Among them, biological treatment is an attractive approach for removing zearalenone.…”

Biological detoxification of fungal toxins and its use in plant breeding, feed and food production

“…In addition, the transformation products of Streptomyces rimosus and Cunninghamella bainieri, 8'-hydroxy-zearalenone and 2,4-dimethoxyzearalenone, respectively, did not bind to rat oestrogen receptor, indicating a loss of oestrogenicity (El-Sharkawy and Abul-Hajj, 1987b). The most significant detoxification was the cleavage of the lactone bond of zearalenone by the mycoparasite Gliocladium roseum (El-Sharkawy and Abul-Hajj, 1988). The product, consisting of a mixture of two isomeric hydroxyketones, decarboxylated spontaneously, rendering the reaction irreversible.…”