Microbial cellulase production using fruit wastes and its applications in biofuels production

Areeshi, Mohammed Y.

doi:10.1016/j.ijfoodmicro.2022.109814

Cited by 8 publications

(4 citation statements)

References 107 publications

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“…Each condition was evaluated for seven days (168 h) regarding the production of FPase and CMCase. Areeshi (2022) has reviewed the importance of water activity on microbial cultivation in SSF [29]. Generally, an increase in the moisture content causes an increase in enzyme production up to some level, when the addition of more water hinders the process.…”

Section: Production Of Cellulasesmentioning

confidence: 99%

“…Furthermore, few companies control the production of cellulases, marketing their cocktail with hidden formulations and under exclusive licensors [28]. The use of cheap substrates, e.g., lignocellulosic wastes, to produce cellulases through a cost-effective process, such as SSF, might make the enzyme less expensive, positively affecting the economics of biofuels [29].…”

Section: Introductionmentioning

confidence: 99%

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Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Campos,

Asevedo,

Souza Filho

et al. 2024

Biomass

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The industrial processing of coconut to produce valuable foods, such as water and milk, generates large volumes of waste, especially the fruit shell. Despite this, material can be used in bioprocess applications, e.g., the production of enzymes, its recalcitrance hinders the cultivation of microorganisms, and low productivity is usually achieved. In this study, the production of cellulolytic enzymes through solid-state fermentation (SSF) and their extraction was investigated using the green coconut fiber pretreated by steam explosion, followed by alkali. The fungus Trichoderma reesei CCT-2768 was cultivated, using an experimental design, to study the effect of the water activity and the amount of biomass in the reactor. The combination of the pretreatment strategies yielded more porous biomass, with less hemicellulose (5.38%, compared to 10.15% of the raw biomass) and more cellulose (47.77% and 33.96% in the pretreated and raw biomasses, respectively). The water activity significantly affected the production of cellulases, with maximum activity yielded at the highest investigated value (0.995). Lastly, the extraction of the enzymes from the cultivation medium was studied, and a 9 g/L NaCl solution recovered the highest CMCase and FPase activities (5.19 and 1.19 U/g, respectively). This study provides an important contribution to the valorization of the coconut residue through (i) the application of the steam explosion technology to optimize the production of cellulases using the SSF technology and (ii) their extraction using different solvents.

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Section: Production Of Cellulasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Campos,

Asevedo,

Souza Filho

et al. 2024

Biomass

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“…Regarding the substrate, diverse types can be used as carbon sources for lignocellulolytic enzyme production, including seeds, fruits, and agro-industrial residues [ 229 , 231 , 232 , 233 , 234 , 235 , 236 , 237 ]. Since it has low cost and wide availability, the use of agro-industrial residues contributes to reducing the production cost of these enzymes, in addition to not competing with the production of food for human and animal consumption [ 238 ].…”

Section: Lignocellulolytic Enzymes Productionmentioning

confidence: 99%

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Benatti

Polizeli

2023

Microorganisms

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Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of lignocellulolytic enzymes for biofuel production, food, bioremediation, and other various industries, is presented as a more sustainable alternative. These enzymes are characterized as a group of enzymes capable of breaking down lignocellulosic biomass into its different monomer units, making it accessible for bioconversion into various products and applications in the most diverse industries. Among all the organisms that produce lignocellulolytic enzymes, microorganisms are seen as the primary sources for obtaining them. Therefore, this review proposes to discuss the fundamental aspects of the enzymes forming lignocellulolytic systems and the main microorganisms used to obtain them. In addition, different possible industrial applications for these enzymes will be discussed, as well as information about their production modes and considerations about recent advances and future perspectives in research in pursuit of expanding lignocellulolytic enzyme uses at an industrial scale.

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“…Cellulase (Cel), as a pivotal industrial enzyme, catalyzes the decomposition of renewable lignocellulosic biomass into oligosaccharides or monosaccharides, which have been explored in numerous industries, such as textile, pulp and paper, detergent, food, and biofuel production ( Ejaz et al, 2021 ; Areeshi, 2022 ). However, there are very limited reports on the synthesis and application of cellulase mediated nanostructure.…”

Section: Introductionmentioning

confidence: 99%

Dual-functional cellulase-mediated gold nanoclusters for ascorbic acid detection and fluorescence bacterial imaging

Wang,

Fang,

Tang

et al. 2023

Front. Bioeng. Biotechnol.

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Protein-protected metal nanomaterials are becoming the most promising fluorescent nanomaterials for biosensing, bioimaging, and therapeutic applications due to their obvious fluorescent molecular properties, favorable biocompatibility and excellent physicochemical properties. Herein, we pioneeringly prepared a cellulase protected fluorescent gold nanoclusters (Cel-Au NCs) exhibiting red fluorescence under the excitation wavelength of 560 nm via a facile and green one-step method. Based on the fluorescence turn-off mechanism, the Cel-Au NCs were used as a biosensor for specificity determination of ascorbic acid (AA) at the emission of 680 nm, which exhibited satisfactory linearity over the range of 10–400 µM and the detection limit of 2.5 µM. Further, the actual sample application of the Au NCs was successfully established by evaluating AA in serum with good recoveries of 98.76%–104.83%. Additionally, the bacteria, including gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli), were obviously stained by Cel-Au NCs with strong red emission. Thereby, as dual-functional nanoclusters, the prepared Cel-Au NCs have been proven to be an excellent fluorescent bioprobe for the detection of AA and bacterial labeling in medical diagnosis and human health maintenance.

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Microbial cellulase production using fruit wastes and its applications in biofuels production

Cited by 8 publications

References 107 publications

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Dual-functional cellulase-mediated gold nanoclusters for ascorbic acid detection and fluorescence bacterial imaging

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