Microbial Cells as Biosorbents for Heavy Metals: Accumulation of Uranium by
            <i>Saccharomyces cerevisiae</i>
            and
            <i>Pseudomonas aeruginosa</i>

Strandberg, G.W.; Shumate, S.E.; Parrott, J.R.

doi:10.1128/aem.41.1.237-245.1981

Cited by 409 publications

(95 citation statements)

References 14 publications

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“…It is known that the cell walls of Mucorales, of which Rhizo-lmS is a genus, are very sensitive to the medium composition and conditions of growth [26]. There have been indications that different media as well as growth conditions produce different culture growth characteristics and different metal sequestering abilities [13,20]. Similar trends could bc observed in the example of the present work.…”

Section: Discussionsupporting

confidence: 85%

“…Even the age of the culture has been reported to have a significant effect on the sequestering ability of the same microorganism [17]. Indications to this extent could be seen in earlier observations of Saccharomyces cererisiae whereby some cells contained deposits of uranium after exposure to the metal-containing solution, whilst others did not [20]. However, these observations may have been incomplete due to the uncontrolled time of exposure as noted recently [21].…”

Section: Discussionmentioning

confidence: 95%

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Advances in biosorption of metals: Selection of biomass types

Volesky¹

1994

FEMS Microbiology Reviews

109

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Within the past decade, the potential of metal biosorption has been well established. For economic reasons, of particular interest are abundant biomass types either generated as a waste by-product of large-scale industrial fermentations or certain metal-binding algae found in large quantities in the sea. Some of these high metal-sorbing biomass types serve as a basis for newly developed metal biosorption processes foreseen particularly as a very competitive means for detoxification of metal-bearing industrial effluents. Ions of lead and cadmium, for instance, have been found to be bound very efficiently from very dilute solutions by the dried biomass of some ubiquitous brown marine algae such as Ascophyllum and Sargassum which accumulate more than 30% of biomass dry weight in the metal. Mycelia of industrially steroid-transforming fungi Rhizopus and Absidia are excellent biosorbents for lead, cadmium, copper, zinc, and uranium, binding also other heavy metals up to 25% of the biomass dry weight. The common yeast Saccharomyces cerevisiae is a 'mediocre' metal biosorbent. Construction of biosorption isotherm curves serves as a basic technique assisting in evaluation of the metal uptake by different biosorbents. The methodology is based on batch equilibrium sorption experiments extensively used for screening and quantitative comparison of new biosorbent materials. Experimental methodologies used in the study of biosorption and selected recent research results demonstrate the route to novel biosorbent materials some of which can even be repeatedly regenerated for re-use.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 95%

Advances in biosorption of metals: Selection of biomass types

Volesky¹

1994

FEMS Microbiology Reviews

109

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“…4), the increase of nickel and zinc removal by dead cells could most likely be attributed to the exposition of further metal-binding sites present inside the cells. According to this hypothesis, an increase in the Cu 2+ accumulation by Penicillium spinulosum and of U accumulation by S. cerevisiae cells permeabilized by the action of detergents (Gadd 1990) or by the action of HCHO or HgCl 2 (Strandberg et al 1981), respectively, was also described.…”

Section: Figurementioning

confidence: 99%

Removal of heavy metals using a brewer’s yeast strain ofSaccharomyces cerevisiae: advantages of using dead biomass

Machado

Janssens

Soares

et al. 2009

Journal of Applied Microbiology

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Aim: The capacities of live and heat‐killed cells of Saccharomyces cerevisiae at 45°C for the removal of copper, nickel and zinc from the solution were compared. Methods and Results: Kinetic studies have shown a maximum accumulation of Ni2+ and Zn2+ after 10 min for both types of cells, while for Cu2+ this was attained after 30 and 60 min for dead and live cells, respectively. Equilibrium studies have shown that inactivated biomass displayed a greater Zn2+ and Ni2+ accumulation than live yeasts. For Cu2+, live and dead cells showed similar accumulation. Fluorescence, scanning electron microscopy and infrared spectroscopy studies have shown that no appreciable structural or molecular changes occurred in the cells during the killing process. The increased metal uptake observed in dead cells can be most likely explained by the loss of membrane integrity, which allows the exposition of further metal‐binding sites present inside the cells. Conclusions: Heat‐killed cells showed a higher degree of heavy metal removal than live cells, being more suitable for further bioremediation works. Significance and Impact of the Study: Dead flocculent cells can be used in a low cost technology for detoxifying metal‐bearing effluents as this approach combines an efficient metal removal with the ease of cell separation.

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“…Its composition implies glucan-, chitin-and galactosamine-containing polymers, and a minor amount of proteins. Thus a large number of potential-binding sites are exhibited by free carboxyl, amino, hydroxyl, phosphate and mercapto groups (Strandberg et al, 1981). Binding to the wall, also called biosorption (Gadd, 1993), is a mechanism not depending on the metabolic activity of the fungus, whereas precipitation with excreted substances relies on the activity of the cells.…”

Section: Extracellular Chelation and Cell-wall Bindingmentioning

confidence: 99%

Extracellular and cellular mechanisms sustaining metal tolerance in ectomycorrhizal fungi

Bellion

Courbot

Jacob

et al. 2006

FEMS Microbiology Letters

268

139

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This review focuses on recent evidence that identifies potential extracellular and cellular mechanisms that may be involved in the tolerance of ectomycorrhizal fungi to excess metals in their environment. It appears likely that mechanisms described in the nonmycorrhizal fungal species are used in the ectomycorrhizal fungi as well. These include mechanisms that reduce uptake of metals into the cytosol by extracellular chelation through extruded ligands and binding onto cell-wall components. Intracellular chelation of metals in the cytosol by a range of ligands (glutathione, metallothioneins), or increased efflux from the cytosol out of the cell or into sequestering compartments are also key mechanisms conferring tolerance. Free-radical scavenging capacities through the activity of superoxide dismutase or production of glutathione add another line of defence against the toxic effect of metals.

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Microbial Cells as Biosorbents for Heavy Metals: Accumulation of Uranium by Saccharomyces cerevisiae and Pseudomonas aeruginosa

Cited by 409 publications

References 14 publications

Advances in biosorption of metals: Selection of biomass types

Advances in biosorption of metals: Selection of biomass types

Removal of heavy metals using a brewer’s yeast strain ofSaccharomyces cerevisiae: advantages of using dead biomass

Extracellular and cellular mechanisms sustaining metal tolerance in ectomycorrhizal fungi

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