Microbial Cell Factories for Diol Production

Sabra, Wael; Groeger, Christin; Zeng, An‐Ping

doi:10.1007/10_2015_330

Cited by 36 publications

(37 citation statements)

References 115 publications

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“…The final concentration of 2,3-BD in the fermentation broths can be in the range 50-150 g L −1 with different types of micro-organisms, substrates, and fermentation modes. 4,25 Therefore, various aqueous 2,3-BD solutions were used for the salting-out experiments. The 2,3-BD contents of the aqueous solutions were 15%, 10%, and 5%, respectively.…”

Section: Salting-out Experimentsmentioning

confidence: 99%

“…The purification of 2,3-BD from the fermentation broth includes several separation steps, such as the separation of solids, the removal of residual salts, the reduction in the excess amount of water in the broth, and distillation. 4 Downstream processing contributes up to 50-70% of the total product cost, 5 which restricts its industrialized development. Thus, one of the focuses of downstream processing is on low-cost technologies.…”

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confidence: 99%

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Salting‐out of bio‐based 2,3‐butanediol from aqueous solutions

Xie

Zhang

Zhou

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND: Microbial production of 2,3-butanediol (2,3-BD) with different substrates and organisms results in various 2,3-BD concentration in fermentation broths. The salting-out of 2,3-BD from different aqueous solutions with different solute content by four high-solubility salts (K 4 P 2 O 7 •3H 2 O, K 2 HPO 4 •3H 2 O, K 2 CO 3 , K 3 PO 4 •3H 2 O) were investigated at 298.15 K. RESULTS:The distribution coefficient and selectivity coefficient of 2,3-BD were greatly affected by the salt composition and the initial salt concentration, but influenced by the solute content slightly. When the initial K 4 P 2 O 7 concentration was higher than or equal to 550 g kg −1 , 100% of 2,3-BD from the aqueous solutions was recovered, and more than 97.5% of water was removed. CONCLUSION:The salting-out effects of four salts on the aqueous 2,3-BD solutions were determined in the following order: K 4 P 2 O 7 >K 3 PO 4 > K 2 HPO 4 >K 2 CO 3 . Moreover, the solubility of 2,3-BD in both phases can be predicted in terms of the initial salt concentration and the solute content. EXPERIMENTAL Materials 2,3-butanediol (2,3-BD) with a purity of 98 wt% was supplied by Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). The purity of 2,3-BD was measured through gas chromatography (GC). Potassium pyrophosphate trihydrate J Chem Technol Biotechnol 2017; 92: 122-132 /jctb where M is the molar mass, for K 4 P 2 O 7 , M = 330.33 g mol −1 , for K 2 HPO 4 , M = 174.18 g mol −1 , for K 3 PO 4 , M = 212.27 g mol −1 , for K 2 CO 3 , M =138.21 g mol −1 , 31 is the mass fraction of salt in the J Chem Technol Biotechnol 2017; 92: 122-132

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Section: Salting-out Experimentsmentioning

confidence: 99%

mentioning

confidence: 99%

Salting‐out of bio‐based 2,3‐butanediol from aqueous solutions

Xie

Zhang

Zhou

et al. 2016

J of Chemical Tech & Biotech

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“…Industrially, E. coli has also been used for the production of 1,3-propanediol by DuPont and 1,4-butanediol by Genomatica (Sabra et al, 2016). 1,3-Propanediol, a monomer for the synthesis of industrial polymers such as polytrimethylene terephthalate, can be naturally produced from glycerol by some microorganisms.…”

Section: Escherichia Colimentioning

confidence: 99%

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Calero

Nikel

2018

Microbial Biotechnology

229

184

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The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful of bacteria have attained the acceptance and widespread use that are needed to fulfil the needs of industrial bioproduction - and only for the synthesis of very few, structurally simple compounds. One of the reasons for this unfortunate circumstance has been the dearth of tools for targeted genome engineering of bacterial chassis, and, nowadays, synthetic biology is significantly helping to bridge such knowledge gap. Against this background, in this review, we discuss the state of the art in the rational design and construction of robust bacterial chassis for metabolic engineering, presenting key examples of bacterial species that have secured a place in industrial bioproduction. The emergence of novel bacterial chassis is also considered at the light of the unique properties of their physiology and metabolism, and the practical applications in which they are expected to outperform other microbial platforms. Emerging opportunities, essential strategies to enable successful development of industrial phenotypes, and major challenges in the field of bacterial chassis development are also discussed, outlining the solutions that contemporary synthetic biology-guided metabolic engineering offers to tackle these issues.

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“…Vicinal diols have broad applications as chemical feedstocks and fuels. They are produced industrially, largely from petroleum through a series of steps including cracking to alkenes, alkene epoxidation, and hydrolysis (Sabra et al, 2016). Ethylene glycol is of particular interest due to its many uses such as polyester fibers, PET (polyethylene terephthalate) plastics, and antifreeze.…”

Section: Introductionmentioning

confidence: 99%

“…Butanediols such as 2,3-butanediol serve as important feedstocks for rubbers, plastics, polymers, pharmaceuticals and insecticides (Gräfje et al, 2000). Ethylene glycol, 1,2propanediol, 1,3-propanediol, 1,4-butanediol and 2,3-butanediol have been biologically produced from sugars (Burgard et al, 2016;Cam et al, 2015;Chen et al, 2016;Nakamura and Whited, 2003;Pereira et al, 2016;Pereira et al, 2015;Sabra et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Biological conversion of gaseous alkenes to liquid chemicals

Desai

Koryakina

Case

et al. 2016

Metabolic Engineering

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Industrial gas-to-liquid (GTL) technologies are well developed. They generally employ syngas, require complex infrastructure, and need high capital investment to be economically viable. Alternatively, biological conversion has the potential to be more efficient, and easily deployed to remote areas on relatively small scales for the utilization of otherwise stranded resources. The present study demonstrates a novel biological GTL process in which engineered Escherichia coli converts C2-C4 gaseous alkenes into liquid diols. Diols are versatile industrially important chemicals, used routinely as antifreeze agents, polymer precursors amongst many other applications. Heterologous co-expression of a monooxygenase and an epoxide hydrolase in E. coli allows whole cell conversion of C2-C4 alkenes for the formation of ethylene glycol, 1,2-propanediol, 1,2-butanediol, and 2,3-butanediol at ambient temperature and pressure in one pot. Increasing intracellular NADH supply via addition of formate and a formate dehydrogenase increases ethylene glycol production titers, resulting in an improved productivity of 9mg/L/h and a final titer of 250mg/L. This represents a novel biological method for GTL conversion of alkenes to industrially valuable diols.

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Microbial Cell Factories for Diol Production

Cited by 36 publications

References 115 publications

Salting‐out of bio‐based 2,3‐butanediol from aqueous solutions

Salting‐out of bio‐based 2,3‐butanediol from aqueous solutions

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Biological conversion of gaseous alkenes to liquid chemicals

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