Microbial Bioprocess for Extracellular Squalene Production and Formulation of Nanoemulsions

Park, Jaehyun; Kang, Dong Hun; Woo, Han Min

doi:10.1021/acssuschemeng.1c05453

Cited by 9 publications

(21 citation statements)

References 53 publications

(79 reference statements)

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“…glutamicum strains using pHsSQS-DIIDF. To improve squalene production, the native decaprenoxanthin biosynthesis has been blocked by mutating idsA ::E16Ter and crtE ::D21Ter to redirect carbon flux toward squalene biosynthesis. , Thus, DM1919 was engineered by the same strategy, and a strain DM1919-Δcrt was constructed. The squalene production of DM1919-Δcrt pHsSQS-DIIDF (5.37 ± 0.51 g/L l -lysine and 118.15 ± 3.30 mg/L) resulted in 1.7-fold production of DM1919 pHsSQS-DIIDF (4.90 ± 0.13 g/L l -lysine and 69.49 ± 4.02 mg/L) (Figure C).…”

Section: Resultsmentioning

confidence: 99%

“…For squalene biosynthesis, ispD and ispF were cloned into the plasmid with truncated FDFT1 from Homo sapiens (tr2HsSQS), dxs , idi , and ispA . For gene repression, an all-in-one Coryne-CRISPR interference system was used .…”

Section: Methodsmentioning

confidence: 99%

“…A previous study has shown that engineered C. glutamicum with human squalene synthase (SQS) produced heterologous squalene in both the cell and cell medium. 8 For co-production of L-lysine and squalene, C. glutamicum DM1919 (a L-lysine producer, Table 1) was transformed with expression vectors that harbor SQS and key MEP genes. A plasmid of pHsSQS-DII expresses truncated human squalene synthase (HsSQS) and the MEP genes of the native dxs encoding for 1-deoxy-D-xylulose 5phosphate (DXP) synthase, idi encoding for isopentenyl diphosphate (IPP) isomerase, and ispA encoding for farnesyl diphosphate synthase from E. coli.…”

Section: Co-production Of Lysine and Squalene Using Crispr Interferen...mentioning

confidence: 99%

“…Successfully engineered C. glutamicum has produced heterologous squalene by expressing heterologous human squalene synthase and overexpressing the MEP pathway genes. ,, …”

Section: Introductionmentioning

confidence: 99%

“…Successfully engineered C. glutamicum has produced heterologous squalene by expressing heterologous human squalene synthase and overexpressing the MEP pathway genes. 4,8,9 Co-production of cell-bound carotenoids (p-carotene or astaxanthin) and secreted lysine in C. glutamicum has been demonstrated in a spray-dried fermentation to valorize the cell biomass. 10 As alternative co-production with amino acids, an in situ solvent overlay fermentation of heterologous and hydrophobic squalene, was selected to increase the value of current amino acid fermentation by harnessing an amino acid producer.…”

Section: Introductionmentioning

confidence: 99%

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Co-production of l-Lysine and Heterologous Squalene in CRISPR/dCas9-Assisted Corynebacterium glutamicum

Park

Woo

2022

J. Agric. Food Chem.

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Corynebacterium glutamicum is widely used for a large-scale industrial producer of feed additive amino acids, such as Llysine. Moreover, C. glutamicum has been engineered for producing various non-native chemicals, including terpenes. For the first time, C. glutamicum was engineered for co-production of L-lysine and heterologous squalene. To control metabolic fluxes for either the L-lysine biosynthesis pathway or the squalene biosynthesis pathway, pyruvate, an intermediate in the central metabolism, a node was regulated by a clustered regularly interspaced short palindromic repeat (CRISPR) interference system. Repressing pyc encoding for pyruvate carboxylase in the L-lysine producer (DM1919) and its derivatives resulted in 99.24 ± 7.63 mg/L total squalene and 6.25 ± 0.20 g/L extracellular lysine at 120 h. Furthermore, various oil overlays were tested for efficient co-productions. In situ extraction with corn oil (10%, v/v) exhibited a separation of 99.75% (w/v) of total squalene (intra-and extracellular squalene), while L-lysine can be secreted in the medium. This co-production strategy will help a potential bioprocess of amino acid production with various terpenes.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Co-production Of Lysine and Squalene Using Crispr Interferen...mentioning

confidence: 99%

“…Successfully engineered C. glutamicum has produced heterologous squalene by expressing heterologous human squalene synthase and overexpressing the MEP pathway genes. ,, …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Co-production of l-Lysine and Heterologous Squalene in CRISPR/dCas9-Assisted Corynebacterium glutamicum

Park

Woo

2022

J. Agric. Food Chem.

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An Ultrafast and Stable Li‐Metal Battery Cycled at −40 °C

Cheng

Wang

Yang

et al. 2022

Adv Funct Materials

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Li-metal battery (LMB) suffers from the unexpected Li dendrite growth and unstable solid-electrolyte interphase (SEI), especially in the extreme conditions, such as high rates and low temperatures (LT). Herein, a high-rate and stable LT LMB is realized by regulating electrolyte chemistry. A weak Li + -solvating solvent 2-methyltetrahydrofuran is used as electrolyte solvent to mitigate the kinetic barrier for Li + de-solvation. Moreover, a co-solvent tetrahydrofuran with a high donor number is incorporated to improve the LT solubility of Li salts, achieving an improved ionic conductivity while maintaining the weak Li + -solvation effect. Furthermore, abundant FSI − anions in contact-ion pairs are presented, facilitating the formation of a stable LiFenriched SEI. Consequently, the Li||Li battery can be operated at 10 mA cm −2 with a small polarization of 154 mV at −40 °C. Meanwhile, an outstanding cumulative cycling capacity of 4000 mAh cm −2 at 8.0 mA cm −2 is achieved, reaching a record high level in LT alkali metal symmetric batteries. Also, rechargeable high-rate and stable full batteries are achieved at −40 °C. This work demonstrates the superiority of electrolyte chemistry for synergistic regulation of both ion transfer kinetics and SEI toward ultrafast and stable rechargeable LMBs at LT.

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Synthesis of Iron Sulfide Nanocrystals Encapsulated in Highly Porous Carbon‐Coated CNT Microsphere as Anode Materials for Sodium‐Ion Batteries

Kim,

Seo,

Kim

et al. 2023

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Highly porous carbon materials with a rationally designed pore structure can be utilized as reservoirs for metal or nonmetal components. The use of small‐sized metal or metal compound nanoparticles, completely encapsulated by carbon materials, has attracted significant attention as an effective approach to enhancing sodium ion storage properties. These materials have the ability to mitigate structural collapse caused by volume expansion during the charging process, enable short ion transport length, and prevent polysulfide elution. In this study, a concept of highly porous carbon‐coated carbon nanotube (CNT) porous microspheres, which serve as excellent reservoir materials is suggested and a porous microsphere is developed by encapsulating iron sulfide nanocrystals within the highly porous carbon‐coated CNTs using a sulfidation process. Furthermore, various sulfidation processes to determine the optimal method for achieving complete encapsulation are investigated by comparing the morphologies of diverse iron sulfide‐carbon composites. The fully encapsulated structure, combined with the porous carbon, provides ample space to accommodate the significant volume changes during cycling. As a result, the porous iron sulfide‐carbon‐CNT composite microspheres exhibited outstanding cycling stability (293 mA h g−1 over 600 cycles at 1 A g−1) and remarkable rate capability (100 mA h g−1 at 5 A g−1).

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Microbial Bioprocess for Extracellular Squalene Production and Formulation of Nanoemulsions

Cited by 9 publications

References 53 publications

Co-production of l-Lysine and Heterologous Squalene in CRISPR/dCas9-Assisted Corynebacterium glutamicum

Co-production of l-Lysine and Heterologous Squalene in CRISPR/dCas9-Assisted Corynebacterium glutamicum

An Ultrafast and Stable Li‐Metal Battery Cycled at −40 °C

Synthesis of Iron Sulfide Nanocrystals Encapsulated in Highly Porous Carbon‐Coated CNT Microsphere as Anode Materials for Sodium‐Ion Batteries

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