Microbial biofilms are shaped by the constant dialogue between biological and physical forces in the extracellular matrix

Wong, Lan Li; Mugunthan, Sudarsan; Kundukad, Binu; Ho, Jia Shin; Rice, Scott; Hinks, Jamie; Seviour, Thomas; Parikh, Atul N.; Kjelleberg, Staffan

doi:10.1111/1462-2920.16306

Cited by 8 publications

(6 citation statements)

References 80 publications

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“…ECM is an important feature of biofilm, which facilitates bacterial aggregation and confers survival benefits on biofilm colonies. 37 This study assessed the impact of EVs addition at various stages on the development of S. aureus biofilm through colonization number detection, crystal violet quantification, and the Congo red assay. The findings revealed a significant reduction in the number of biofilm colonization, total biofilm, and ECM content when EVs were introduced prior to biofilm formation.…”

Section: ■ Discussionmentioning

confidence: 99%

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Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation

Liu,

Guo,

et al. 2024

ACS Infect. Dis.

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The colonizing microbiota on the body surface play a crucial role in barrier function. Staphylococcus aureus (S. aureus) is a significant contributor to skin infection, and the utilization of colonization resistance of skin commensal microorganisms to counteract the invasion of pathogens is a viable approach. However, most studies on colonization resistance have focused on skin bacteria, with limited research on the resistance of skin fungal communities to pathogenic bacteria. Extracellular vehicles (EVs) play an important role in the colonization of microbial niches and the interaction between distinct strains. This paper explores the impact of Malassezia restricta (M. restricta), the fungus that dominates the normal healthy skin microbiota, on the proliferation of S. aureus by examining the distribution disparities between the two microorganisms. Based on the extraction of EVs, the bacterial growth curve, and biofilm formation, it was determined that the EVs of M. restricta effectively suppressed the growth and biofilm formation of S. aureus. The presence of diverse metabolites was identified as the primary factor responsible for the growth inhibition of S. aureus, specifically in relation to glycerol phospholipid metabolism, ABC transport, and arginine synthesis. These findings offer valuable experimental evidence for understanding microbial symbiosis and interactions within healthy skin.

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Section: ■ Discussionmentioning

confidence: 99%

“…Crystal violet quantification is commonly used to evaluate biofilm formation. ECM is an important feature of biofilm, which facilitates bacterial aggregation and confers survival benefits on biofilm colonies . This study assessed the impact of EVs addition at various stages on the development of S.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation

Liu,

Guo,

et al. 2024

ACS Infect. Dis.

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“…polysaccharides, proteins, lipids, and extracellular DNA) that holds them together and enables the establishment of many of the emergent properties of biofilms. A constant and dynamic dialogue between the biological and physical forces modulated by microbiome members and host/environment have been suggested to shape and maintain microbial biofilms (Wong et al 2023 ).…”

Section: Biofilm Formationmentioning

confidence: 99%

Life at the borderlands: microbiomes of interfaces critical to One Health

Law,

Mathes,

Paten

et al. 2024

FEMS Microbiology Reviews

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Microbiomes are foundational components of the environment that provide essential services relating to food security, carbon sequestration, human health, and the overall well-being of ecosystems. Microbiota exert their effects primarily through complex interactions at interfaces with their plant, animal, and human hosts, as well as within the soil environment. This review aims to explore the ecological, evolutionary, and molecular processes governing the establishment and function of microbiome-host relationships, specifically at interfaces critical to One Health – a transdisciplinary framework that recognises that the health outcomes of people, animals, plants and the environment are tightly interconnected. Within the context of One Health, the core principles underpinning microbiome assembly will be discussed in detail, including biofilm formation, microbial recruitment strategies, mechanisms of microbial attachment, community succession and the effect these processes have on host function and health. Finally, this review will catalogue recent advances in microbiology and microbial ecology methods that can be used to profile microbial interfaces, with particular attention to multi-omic, advanced imaging, and modelling approaches. These technologies are essential for delineating the general and specific principles governing microbiome assembly and functions, mapping microbial interconnectivity across varying spatial and temporal scales, and for the establishment of predictive frameworks that will guide the development of targeted microbiome-interventions to deliver One Health outcomes.

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“…However, whether a biofilm forms at a particular site in the host also depends on the physiochemical characteristics of the microenvironment. Physical forces that affect biofilm formation have been reviewed in greater depth elsewhere ( Renner and Weibel, 2011 ; Arias and Brito, 2021 ; Wong et al., 2023 ), but two major factors that are especially relevant to in vivo environments are fluid flow and viscoelasticity of the host substrate.…”

Section: Intrinsic and Extrinsic Factors Affecting Tissue-associated ...mentioning

confidence: 99%

Bacterial biofilms in the human body: prevalence and impacts on health and disease

Perry,

Tan

2023

Front. Cell. Infect. Microbiol.

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Bacterial biofilms can be found in most environments on our planet, and the human body is no exception. Consisting of microbial cells encased in a matrix of extracellular polymers, biofilms enable bacteria to sequester themselves in favorable niches, while also increasing their ability to resist numerous stresses and survive under hostile circumstances. In recent decades, biofilms have increasingly been recognized as a major contributor to the pathogenesis of chronic infections. However, biofilms also occur in or on certain tissues in healthy individuals, and their constituent species are not restricted to canonical pathogens. In this review, we discuss the evidence for where, when, and what types of biofilms occur in the human body, as well as the diverse ways in which they can impact host health under homeostatic and dysbiotic states.

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Microbial biofilms are shaped by the constant dialogue between biological and physical forces in the extracellular matrix

Cited by 8 publications

References 80 publications

Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation

Extracellular Vehicles from Commensal Skin Malassezia restricta Inhibit Staphylococcus aureus Proliferation and Biofilm Formation

Life at the borderlands: microbiomes of interfaces critical to One Health

Bacterial biofilms in the human body: prevalence and impacts on health and disease

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