Microbial Bio-production of Proteins and Valuable Metabolites

Johnson, Abiya; Deshmukh, P.B.; Kaushik, Shubhangi; Sharma, Vikas

doi:10.1007/978-981-13-8391-5_15

Cited by 3 publications

(2 citation statements)

References 192 publications

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“…With further development, PicoShells could be designed to quantify single-cell protein secretions, an important measurement for microbial recombinant protein production [ 27 , 28 ]. Antibodies specific for the secreted proteins of interest can be conjugated to the particle’s hydrogel shell to bind the secretion and prevent diffusion out of the particle, and secretions can be tagged with fluorescent secondary antibodies [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Enabling Clonal Analyses of Yeast in Outer Space by Encapsulation and Desiccation in Hollow Microparticles

Williamson

Zee

et al. 2022

Life

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Studying microbes at the single-cell level in space can accelerate human space exploration both via the development of novel biotechnologies and via the understanding of cellular responses to space stressors and countermeasures. High-throughput technologies for screening natural and engineered cell populations can reveal cellular heterogeneity and identify high-performance cells. Here, we present a method to desiccate and preserve microbes in nanoliter-scale compartments, termed PicoShells, which are microparticles with a hollow inner cavity. In PicoShells, single cells are confined in an inner aqueous core by a porous hydrogel shell, allowing the diffusion of nutrients, wastes, and assay reagents for uninhibited cell growth and flexible assay protocols. Desiccated PicoShells offer analysis capabilities for single-cell derived colonies with a simple, low resource workflow, requiring only the addition of water to rehydrate hundreds of thousands of PicoShells and the single microbes encapsulated inside. Our desiccation method results in the recovery of desiccated microparticle morphology and porosity after a multi-week storage period and rehydration, with particle diameter and porosity metrics changing by less than 18% and 7%, respectively, compared to fresh microparticles. We also recorded the high viability of Saccharomyces cerevisiae yeast desiccated and rehydrated inside PicoShells, with only a 14% decrease in viability compared to non-desiccated yeast over 8.5 weeks, although we observed an 85% decrease in initial growth potential over the same duration. We show a proof-of-concept for a growth rate-based analysis of single-cell derived colonies in rehydrated PicoShells, where we identified 11% of the population that grows at an accelerated rate. Desiccated PicoShells thus provide a robust method for cell preservation before and during launch, promising a simple single-cell analysis method for studying heterogeneity in microbial populations in space.

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Section: Discussionmentioning

confidence: 99%

Enabling Clonal Analyses of Yeast in Outer Space by Encapsulation and Desiccation in Hollow Microparticles

Williamson

Zee

et al. 2022

Life

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“…In contrast, microbial synthesis, as a more competitive and emerging approach, is a hot research topic nowadays, and this method has good sustainability, high productivity, low production cost, and high scalability. It can also effectively address the concerns about rapid global population growth and climate change [ 9 , 10 ]. Therefore, the use of microbial methods for MA synthesis will contribute to the green development of the industry, increase the sustainability of the synthesis process, reduce the cost of MA production, and improve production efficiency [ 4 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Revealing the Mechanism of Aroma Production Driven by High Salt Stress in Trichomonascus ciferrii WLW

Xian,

Yang,

et al. 2024

Foods

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Douchi is a Chinese traditional fermented food with a unique flavor. Methyl anthranilate (MA) plays an important role in formation of this flavor. However, the complicated relationship between the MA formation and the metabolic mechanism of the key functional microorganisms remains unclear. Here, we elucidated the response mechanism of aroma production driven by high salt stress in Trichomonascus ciferrii WLW (T. ciferrii WLW), which originates from the douchi fermentation process. The highest production of MA was obtained in a 10% NaCl environment. The enhanced expression of the key enzyme genes of the pentose phosphate pathway and shikimic acid pathway directed carbon flow toward aromatic amino acid synthesis and helped sustain an increased expression of metK to synthesize a large amount of the methyl donor S-adenosylmethionine, which promoted methyl anthranilate yield. This provides a theoretical basis for in-depth research on the applications of the flavor formation mechanisms of fermented foods.

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Evaluation of the Role of Probiotics As a New Strategy to Eliminate Microbial Toxins: a Review

Ghadaksaz

Nodoushan

Sedighian

et al. 2022

Probiotics & Antimicro. Prot.

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Microbial Bio-production of Proteins and Valuable Metabolites

Cited by 3 publications

References 192 publications

Enabling Clonal Analyses of Yeast in Outer Space by Encapsulation and Desiccation in Hollow Microparticles

Enabling Clonal Analyses of Yeast in Outer Space by Encapsulation and Desiccation in Hollow Microparticles

Revealing the Mechanism of Aroma Production Driven by High Salt Stress in Trichomonascus ciferrii WLW

Evaluation of the Role of Probiotics As a New Strategy to Eliminate Microbial Toxins: a Review

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