Microbial Association with Genus Actinomyces in Primary and Secondary Endodontic Lesions, Review

Dioguardi, Mario; Quarta, Cristian; Alovisi, Mario; Crincoli, Vito; Aiuto, Riccardo; Crippa, Rolando; Angiero, Francesca; Laneve, Enrica; Sovereto, Diego; Lillo, Alfredo De; Troiano, Giuseppe; Muzio, Lorenzo Lo

doi:10.3390/antibiotics9080433

Cited by 12 publications

(10 citation statements)

References 69 publications

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“…Fusobacterium and Porphyromonas were found to be associated with symptoms in previous studies (Gomes et al, 2021). Sphingomonas which were significantly higher relative abundances in asymptomatic samples were also detected in periapical lesions (Dioguardi et al, 2020).…”

Section: Discussionmentioning

confidence: 90%

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

et al. 2021

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Aim: To determine the microorganism in root canal systems of root filled teeth with periapical disease and their relationship with clinical symptoms using nextgeneration sequencing. Methodology:The roots of 10 extracted teeth were collected from 10 patients who presented with post-treatment apical periodontitis (PTAP; six with symptoms and four without symptoms). Each root was divided horizontally into two parts (apical and coronal segments) and cryo-pulverized. Microbial communities were detected using 16S rDNA hypervariable V3-V4 region. The diversity, principal coordinate analysis and linear discriminant analysis effect size were performed in the symptomatic and asymptomatic groups (apical and coronal parts respectively). A Mann-Whitney test and an analysis of similarities were applied for intergroup analysis, at a significance level of 5%.Results: A total of 23 phyla, 257 genera and 425 species were detected. Firmicutes was the most abundant phylum in all samples. Three phyla (Fusobacteria, Synergistetes and unidentified_Bacteria) and seven genera (Fusobacterium, Porphyromonas, Phocaeicola, Olsenella, Campylobacter, Tannerella and Fretibacterium) were significantly more abundant in the symptomatic patients (p < .05), whereas asymptomatic patients had more Sphingomonas. The species more significantly abundant in the symptomatic samples were Porphyromonas gingivalis, Phocaeicola abscessus, Campylobacter showae, Tannerella forsythia and Olsenella uli (p < .05).Conclusions: A greater microbial diversity was observed in root filled teeth with PTAP compared to earlier reports. Several genera and species in root canal systems might be associated with clinical symptoms of PTAP.

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Section: Discussionmentioning

confidence: 90%

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

et al. 2021

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“…Infection involving the cervicofacial area is most common, followed by abdominal and pelvic involvement and thoracic involvement [ 6 ]. The loss of the integrity of the mucous membrane of the oral cavity, caused by extractions, endodontic treatment, bone and dental fractures, and periodontal disease can cause infection with these microorganisms [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Abdominopelvic Actinomycosis—The Diagnostic and Therapeutic Challenge of the Most Misdiagnosed Disease

Vasilescu

Târcoveanu

Lupașcu

et al. 2022

Life

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Abdominopelvic actinomycosis is a rare chronic or subacute bacterial infection caused by Actinomyces israelii, a Gram-positive anaerobic bacterium that normally colonizes the digestive and genital tracts, clinically presented as an inflammatory mass or abscess formation. Methods: We reviewed the medical records of the patients from our clinic with abdominopelvic actinomycosis who underwent surgery between 2002 and 2022. In this period, 28 cases (9 men and 19 women) were treated. The mean age was 43.36 years and they were hospitalized for abdominopelvic tumors or inflammatory tumors in 15 cases and inflammatory disease in 13 cases. Results: Causes of actinomycosis in the studied group were an intra-uterine contraceptive device in 17 cases, foreign bodies in 2 cases, diabetes in 4 cases, stenting of the bile duct in 1 case, and immunodepression. For 6 patients, we performed surgery by open approach and for 21 patients by a laparoscopic approach. For nine patients, abdominopelvic actinomycosis had been mimicking a colon malignancy (cecum and ascending colon, four cases; transverse colon, two cases; and on the greater omentum, three cases) and for six patients, a pelvic tumor (advanced ovarian cancer). After surgery the patients underwent specific treatment with antibiotics, with good results. In two cases we discovered and treated hepatic actinomycosis, one case by a laparoscopic approach and one case by a percutaneous approach. In our lot we noticed three recurrences that required reintervention in patients who had had short-term antibiotics due to non-compliance with treatment out of four such cases. Conclusions: For abdominopelvic malignancies, actinomycosis should be included in the differential diagnosis, as well as for inflammatory bowel diseases and bowel obstructions. We have a wide range of patients considering the rarity of this condition. Long-term antibiotics are necessary to prevent recurrence.

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“…play an essential role in the formation of dental biofilms and could contribute to the development of diseases such as caries and periodontitis (de Oliveira et al, 2020). Dioguardi et al (2020) have previously reviewed microbial associations with Actinobacteria in primary endodontic lesions, which indicated the selective conditions of anaerobiosis and the loss of the integrity of the oral mucous membrane could give rise to infection by these microorganisms. In addition, other microbial markers, such as Lactobacillus, TM7, and Rothia, were significantly depleted in the AP group compared with the HO group.…”

Section: Discussionmentioning

confidence: 99%

Distinctive microbiota distribution from healthy oral to post-treatment apical periodontitis

Zhang

Yun

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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Post-treatment apical periodontitis (PoAP) occurs when root canal treatment has not adequately eliminated bacterial invasion and infection. Yet little is known about the bacterial composition and changes related to the etiology and pathogenesis of PoAP. In this study, clinical samples classified as root apex (HARD) and periapical granulation tissues (SOFT) were separately collected from 10 patients with PoAP. The microbiota of each sample was characterized by 16S rRNA gene sequencing, and the obtained dataset was coanalyzed with 20 NCBI sequence read archive (SRA) datasets of healthy oral (HO) and primary apical periodontitis (PAP). We observed 2522 operational taxonomic units (OTUs) belonging to 29 phyla, and all samples shared 86.5% of the sequence reads. The OTUs affiliated with Bacteroidetes, Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria, were identified as core microbiota, which accounted for nearly 90% of 16S rRNA sequences in all samples. However, the principal coordinates analysis (PCoA) of the beta diversity demonstrated that the three periapical statuses have distinct microbial compositions. Compared with HO and PoAP, Actinomyces has a significantly increased abundance in PAP. The microbial diversities in PoAP were significantly lower than those in the HO and PAP (p<0.05). The relative abundance of most bacterial taxa was decreasing, except that Clostridia and Synergistia were increased. Furthermore, we explored the potential metabolic differences of the microbial communities by KEGG pathway prediction. We revealed that the microbiota of PoAP might have a more active metabolic capacity, including carbohydrate metabolism, energy metabolism, and enzyme cofactor/carrier biosynthesis (p<0.05). Our study revealed that invasion of opportunistic pathogens such as Clostridia and Synergistia might play a significant role in PoAP, thus guiding the further study of complex microbial-host interactions and the development of more effective diagnostic and therapeutic methods.

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Microbial Association with Genus Actinomyces in Primary and Secondary Endodontic Lesions, Review

Cited by 12 publications

References 69 publications

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

Abdominopelvic Actinomycosis—The Diagnostic and Therapeutic Challenge of the Most Misdiagnosed Disease

Distinctive microbiota distribution from healthy oral to post-treatment apical periodontitis

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