Microbial and chemical composition of liquid‐associated bacteria in goats' rumen and fermenters

Abecia, Leticia; Soto, E. C.; Ramos-Morales, E.; Molina-Alcaide, E.

doi:10.1111/jpn.12165

Cited by 6 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this was likely due to increasing concentrations of the CP fraction A (43% higher in RCS 60 than in RCS 15 , Table ) that is rapidly converted to NH 3 ‐N in the rumen (Sniffen, O'Connor, van Soest, Fox, & Russell, ), and to a lower incorporation of NH 3 ‐N into microbial protein, reflected in a lower microbial protein synthesis (Table ). A decline in microbial growth with increasing RCS level was not only indicated by a lower N incorporation into LAM and Feed + SAM fractions at RCS 60 compared with RCS 15 , but also by decreasing PB concentrations (Abecia, Soto, Ramos‐Morales, & Molina‐Alcaide, ) of LAM and Feed + SAM with increasing dietary RCS level and a lower mass of both fractions. Such results confirm the findings of Schulz et al.…”

Section: Discussionmentioning

confidence: 95%

In vitro rumen fermentation, microbial protein synthesis and composition of microbial community of total mixed rations replacing maize silage with red clover silage

Castro-Montoya

Witzig

Rahman

et al. 2018

Animal Physiology Nutrition

View full text Add to dashboard Cite

This study aimed to evaluate in vitro fermentation characteristics, microbial protein synthesis and microbial community composition when replacing maize silage (MS) with red clover silage (RCS) in total mixed rations (TMR) of dairy cows. Treatments included TMR containing forage (MS and RCS) and concentrates (0.75:0.25) with targeted proportions (dry matter (DM) basis) of RCS in TMR of 0.15 (RCS ), 0.30 (RCS ), 0.45 (RCS ), and 0.60 (RCS ), in substitution of MS. Samples of the TMR were incubated using the in vitro Ankom RF technique with a mixture of rumen fluid and buffer solution (1:2 v/v) for 8 and 24 hr. Gas production and total short-chain fatty acids concentration did not differ between diets, whereas ammonia-nitrogen concentration increased with increasing level of RCS. Acetate proportion was not affected by RCS level, but propionate showed a linear increase with increasing level of RCS at the expenses of butyrate. Branched fatty acids proportions linearly declined, reflecting a reduced deamination of true protein. Gene copy numbers of protozoa linearly decreased with increasing RCS levels, while total numbers of bacteria and methanogens were not affected by diet. The amylolytic bacteria Ruminobacter amylophillus and Prevotella bryantii showed evidence to increase with higher RCS levels after 8 hr and 24 hr, respectively, whereas no effects of diet where observed for the fibrolytic bacteria Ruminococcus albus, Ruminococcus flavefaciens and Fibrobacter succinogenes. Concentrations of purine bases, and total N production in liquid-associated microbes declined with increasing RCS levels, suggesting a negative impact of this feed on microbial growth. The findings of this study suggest that in general, microbial protein synthesis might be impaired by the substitution of MS by RCS, therefore caution should be taken when formulating diets for dairy cows using high levels of RCS as ingredient.

show abstract

Section: Discussionmentioning

confidence: 95%

In vitro rumen fermentation, microbial protein synthesis and composition of microbial community of total mixed rations replacing maize silage with red clover silage

Castro-Montoya

Witzig

Rahman

et al. 2018

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

“…It is not clear why QTE 15 caused this effect, but changes in PB concentrations may reflect changes in the microbial growth rate. Similar to N content, it might reflect differences in the growth stage of microbes and/or the species composition of the microbial consortium (Abecia, Soto, Ramos‐Morales, & Molina‐Alcaide, ). The latter would mean a faster growth rate for the QTE 15 than the CON and QTE 30 .…”

Section: Discussionmentioning

confidence: 99%

In vitro microbial protein synthesis, ruminal degradation and post‐ruminal digestibility of crude protein of dairy rations containing Quebracho tannin extract

Castro-Montoya

Westreicher-Kristen

Henke

et al. 2017

Animal Physiology Nutrition

View full text Add to dashboard Cite

SummaryThis study evaluated the effects of Quebracho tannin extract (QTE) on in vitro ruminal fermentation, chemical composition of rumen microbes, ruminal degradation and intestinal digestibility of crude protein (iCPd). Three treatments were tested, the control (basal diet without QTE), the basal diet with 15 g QTE/kg dry matter (DM) and the basal diet with 30 g QTE/kg DM. The basal diet contained (g/kg DM): 339 grass silage, 317 maize silage and 344 concentrate. In vitro gas production kinetic was determined using the Hohenheim gas test (Experiment 1). The Ankom RF technique, a batch system with automatic gas pressure recordings, was used to determine in vitro production of shortchain fatty acids (SCFA) and ammonia-nitrogen concentration (NH 3 -N), as well as nitrogen and purine bases content in liquid-associated microbes (LAM) and in a residue of undegraded feed and solid-associated microbes (Feed+SAM) (Experiment 2). Ruminal degradation and iCPd were determined using the nylon bag technique and the mobile nylon bag technique, respectively (Experiment 3). Gas production (Experiment 1), total SCFA and NH 3 -N (Experiment 2) decreased with increasing QTE levels. Microbial mass and composition of LAM were not affected by QTE, but total mass of Feed+SAM linearly increased, likely due to decreased substrate degradation with increasing QTE levels.The total amount of N in microbial mass and undegraded feed after the in vitro incubation increased with increasing QTE levels, suggesting a potential greater N flow from the rumen to the duodenum. In contrast to in vivo studies with the same QTE, no effects were detected on ruminal effective degradability and iCPd, when using the nylon bag techniques. Based on the in vitro procedures, QTE increased the supply of N postrumen; however, some evidence of a decreased fibre degradation were also observed.Therefore, the benefit of adding QTE to diets of cattle is still questionable. disputed. In line with this, particularly about the risk that tannin may actually decrease nutrients absorption in the small intestine (Frutos, Hervás, Giráldez, & Mantecón, 2004) by failing to dissociate from the bound nutrients under the intestinal conditions. On the other hand, by (re-)binding to digestive and microbial enzymes and to microbial protein (Waghorn, 2008). Moreover, CT may also bind to microbes or to their enzymes in the rumen, thus inhibiting microbial growth and reducing microbial CP (MCP) supply to the animal.Recent studies of Ahnert, Dickhoefer, Schulz, and Susenbeth (2015) and Henke et al. (2017)

show abstract

“…The V3 region of the 16S rRNA gene was amplified from the extracted DNA by PCR for DGGE analysis using the bacterial primers (338f forward 5´-CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG GCC TAC GGG AGG CAG CAG-3´ and 534r reverse 5´-ATTACC GCG GCT GCT GG-30 (Muyzer et al 1993). The PCR amplification followed the procedure described by Abecia et al (2014). Briefly, the PCR reaction solution contained 50 ng DNA in a 50 µl mix containing 1 mM buffer, 1.25 mM of each primer, 0.8 mM of dNTPs, 2.5 mM MgCl 2 and 2.5U of Taq DNA polymerase in 10 mM Tris HCl (pH 9.0).…”

Section: Extraction Of Dna and Dgge And Arisa Analysesmentioning

confidence: 99%

Comparison of automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques for analysing the influence of diet on ruminal bacterial diversity

Saro

Molina-Alcaide

Abecia

et al. 2018

Archives of Animal Nutrition

View full text Add to dashboard Cite

The objective of this study was to compare the automated ribosomal intergenic spacer analysis (ARISA) and the denaturing gradient gel electrophoresis (DGGE) techniques for analysing the effects of diet on diversity in bacterial pellets isolated from the liquid (liquid-associated bacteria (LAB)) and solid (solid-associated bacteria (SAB)) phase of the rumen. The four experimental diets contained forage to concentrate ratios of 70:30 or 30:70 and had either alfalfa hay or grass hay as forage. Four rumen-fistulated animals (two sheep and two goats) received the diets in a Latin square design. Bacterial pellets (LAB and SAB) were isolated at 2 h post-feeding for DNA extraction and analysed by ARISA and DGGE. The number of peaks in individual samples ranged from 48 to 99 for LAB and from 41 to 95 for SAB with ARISA, and values of DGGE-bands ranged from 27 to 50 for LAB and from 18 to 45 for SAB. The LAB samples from high concentrate-fed animals tended (p < 0.10) to show greater peak numbers and Shannon index values than those isolated from high forage-fed animals with ARISA, but no differences were identified with DGGE. The SAB samples from high concentrate-fed animals had lower (p < 0.05) peak numbers and Shannon index values than those from animals fed high-forage diets with ARISA, but only a trend was noticed for these parameters with DGGE (p < 0.10). The ARISA detected that animals fed alfalfa hay diets showed lower (p < 0.05) SAB diversity than those fed grass hay diets, but no differences were observed with DGGE. No effect of forage type on LAB diversity was detected by any technique. In this study, ARISA detected some changes in ruminal bacterial communities that were not detected by DGGE, and therefore ARISA was considered more appropriate for assessing bacterial diversity of ruminal bacterial pellets. The results highlight the impact of the fingerprinting technique used to draw conclusions on dietary factors affecting bacterial diversity in ruminal bacterial pellets.

show abstract

Microbial and chemical composition of liquid‐associated bacteria in goats' rumen and fermenters

Cited by 6 publications

References 61 publications

In vitro rumen fermentation, microbial protein synthesis and composition of microbial community of total mixed rations replacing maize silage with red clover silage

In vitro rumen fermentation, microbial protein synthesis and composition of microbial community of total mixed rations replacing maize silage with red clover silage

In vitro microbial protein synthesis, ruminal degradation and post‐ruminal digestibility of crude protein of dairy rations containing Quebracho tannin extract

Comparison of automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques for analysing the influence of diet on ruminal bacterial diversity

Contact Info

Product

Resources

About