Microbial and antibiotic resistant constituents associated with biological aerosols and poultry litter within a commercial poultry house

Brooks, John P.; McLaughlin, M. R.; Scheffler, Brian E.; Miles, D. M.

doi:10.1016/j.scitotenv.2010.06.038

Cited by 72 publications

(86 citation statements)

References 26 publications

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“…Therefore, although air sampling by impingement has been recognised as an appropriate sampler for assessing other airborne microorganisms, it has not been fully validated for airborne Salmonella spp. According to our results, and in agreement with Brooks et al (2010), the use of impingement for quantification or detection of cultivable airborne Salmonella spp. is not recommended.…”

Section: Discussionsupporting

confidence: 92%

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Material particulado y bioaerosoles en el aire de granjas de aves y conejos: cuantificación, caracterización y medidas de reducción

Sales¹

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En su conjunto, los resultados presentados en esta Tesis Doctoral proporcionan una información útil sobre el PM y los bioaerosoles en el aire de alojamientos ganaderos, que permitirá diseñar e implementar medidas de reducción prácticas y eficaces que mejoren la calidad del aire en los alojamientos ganaderos y reduzcan su emisión al exterior.v Abstract Livestock houses are an important source of particulate matter (PM) and bioaerosols. These substances can have a detrimental effect on human and animal health and the environment, as well. Knowledge on the origin of PM and bioaerosols in livestock houses and the factors affecting their generation and aerosolization is necessary to reduce them. The present PhD thesis addresses issues related with the concentration, origin and physical, chemical and biological properties of airborne PM in rabbit and poultry farms, their relationship with pathogenic bioaerosols and techniques to reduce them. This work aims to contribute to alleviate the negative effects of these substances both indoor and outdoor livestock houses.The specific objectives within this PhD thesis were i). to characterize airborne PM in rabbit farms in terms of morphology, chemical composition and bacterial concentration in different size fractions, ii). to quantify airborne PM10 and PM2.5 concentrations and emissions in rabbit farms, and to identify the main factors related with farm activities influencing PM generation, iii). to assess the spatial distribution of mesophilic aerobic bacteria in the air during a broiler cycle and examine their relationship with the concentration and evolution of PM, iv). to compare the performance of techniques to sample and detect airborne Salmonella spp. in broiler farms and v). to evaluate the application of an air disinfectant to reduce airborne microorganism in a commercial laying hen house, with focus on its effect on Mycoplasma spp.The results of this thesis suggest that poultry and rabbit houses generate and emit relevant amounts of PM and bioaerosols which can exceed the limit values established in Directive 2008/50/EC on ambient air quality and cleaner air for Europe, especially in poultry farms. Therefore, these substances must be controlled and reduced to protect the environment and the health and welfare of humans and animals.In rabbit houses, PM showed a complex morphology and chemical composition, being fragmentation type particles with irregular and acute edges, rich in S, Ca, Mg, Na and Cl the most abundant. The concentration of mesophilic aerobic bacteria per cubic meter in the air varied between 3.1x10 3 and 1.6x10 6 colony forming units, CFU. The main sources of PM were skin, animal feed and faeces from cleaning activities, specially sweeping and the animals themselves. The average concentration of PM10 (particles vi smaller than 10 µm in diameter) was 0.08 ± 0.06 mg/m 3 for fattening rabbits and 0.05±0.06 mg/m 3 for reproductive does and the average concentration of PM2.5 (particles smaller than 2.5 µm in diameter) was 0.01±0.02 mg/m 3 for fattening ra...

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Section: Discussionsupporting

confidence: 92%

“…The manipulation and processing of the liquid impingement in the laboratory may also influence the detection of cultivable Salmonella spp. Using impingement, Brooks et al (2010) reported the difficulties in isolating Salmonella spp. from air samples.…”

Section: Discussionmentioning

confidence: 99%

Material particulado y bioaerosoles en el aire de granjas de aves y conejos: cuantificación, caracterización y medidas de reducción

Sales¹

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“…Clostridium in particular contains species that can cause foodborne illnesses in humans and are present in aerosols at dairy, swine, and poultry operations (Brooks et al, 2010;Nehme et al, 2008; Table 3 Phylogenetic affiliations of bacterial 16S rRNA gene sequences obtained from aerosol samples collected downwind from the center pivot irrigation systems using dairy wastewater. Ravva et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation

Dungan

2012

Environment International

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Animal manures are known to harbor a variety of zoonotic pathogens, which are suspected of being transported off-site as aerosols from confined feeding operations. In this study, aerosols were collected using a high-volume sampler downwind from a 10,000 cow open-freestall dairy and nearby fields being sprinkler irrigated with wastewater. DNA extracts were prepared from the aerosol samples, then a region of the16S ribosomal RNA gene was sequenced for bacterial identification and phylogenetic classification. At the dairy and irrigation sites, Proteobacteria (α-, β-, and γ-subdivisions) was the most abundant phylum, representing 78% and 69% of all sequences, respectively, while Actinobacteria, Bacteroidetes and Firmicutes represented only 10% or less of the sequences. Of the 191 clones sequenced from the dairy aerosol samples, 6 sequences were found to be homologous with uncultured bacteria from cow milk, rumen, and fecal samples. However, none of the sequence matches was affiliated with bacteria known to be pathogenic to otherwise healthy humans. Although our results do suggest a high diversity among the aerosolized bacteria, the sampling strategy employed in this study may not account for the variable nature of bioaerosol emissions.

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“…Caroll et al (2016) menyatakan beberapa mekanisme yang menyebabkan bakteri resisten terhadap obat-obatan, antara lain : 1) bakteri memproduksi enzim yang menghancurkan zat aktif yang terdapat dalam obat, 2) bakteri mengubah permeabilitasnya terhadap obat , 3) bakt eri mengembangkan struktur target lain untuk obat, 4) bakteri mengembangkan jalur metabolisme yang terlewati saat terjadi reaksi penghambatan oleh obat, dan 5) bakteri mengembangkan enzim lain yang tetap dapat menjalankan fungsi metabolisnya namun kurang dipengaruhi oleh obat. Dalam hal ini bakteri yang resisten dapat tersebar melalui udara (Brooks, McLaughlin, Schefffler, & Miles, 2010;Gibbs et al, 2006;McEachran, 2015;Rule, Evans, & Silbergeld, 2008;Zhong, Chai, & Duan, 2009), air (Wilson, 2004) dan angin (Marti et al, 2014;Wichmann, UdikovicKolic, Andrew, Handelsman, 2014). Bahkan serangga (Marti, 2013) dan tikus (Literak et al, 2009;van de Giessen et al, 2009) dapat membawa bakteri resisten dari lokasi pertanian.…”

Section: Pola Resistensi Bakteri Terhadap Antibiotikunclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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Microbial and antibiotic resistant constituents associated with biological aerosols and poultry litter within a commercial poultry house

Cited by 72 publications

References 26 publications

Material particulado y bioaerosoles en el aire de granjas de aves y conejos: cuantificación, caracterización y medidas de reducción

Material particulado y bioaerosoles en el aire de granjas de aves y conejos: cuantificación, caracterización y medidas de reducción

Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

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