Microbial adaptation and response to high ammonia concentrations and precipitates during anaerobic digestion under psychrophilic and mesophilic conditions

Quispe-Cardenas, Estefanny; Rogers, Shane

doi:10.1016/j.watres.2021.117596

Cited by 15 publications

(10 citation statements)

References 63 publications

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“…In general, the relative abundance of acetoclastic Archaea ( Methanosaeta , Methanosarcina ) showed a statistically significant difference compared to hydrogenotrophic and methylotrophic Archaea in all other treatments. However, Methanosarcina has been reported to potentially act as a hydrogenotroph under high concentrations of ammonia, thereby probably increasing the activity levels of the specific methanogenic pathway [ 47 , 48 ]. Among the hydrogenotrophic methanogens, members of the Methanomicrobiaceae , Methanobacteriaceae , Methanocorpusculaceae , and Methanospirillaceae families were detected in the AD reactors.…”

Section: Resultsmentioning

confidence: 99%

Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition

et al. 2023

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This study aimed to investigate the role of the bioaugmented critical biomass that should be injected for successful bioaugmentation for addressing ammonia inhibition in anaerobic reactors used for biogas production. Cattle manure was used as a feedstock for anaerobic digestion (AD). A mixed microbial culture was acclimated to high concentrations of ammonia and used as a bioaugmented culture. Different volumes of bioaugmented culture were injected in batch anaerobic reactors under ammonia toxicity levels i.e., 4 g of NH4+-N L−1. The results showed that injecting a volume equal to 65.62% of the total working reactor volume yielded the best methane production. Specifically, this volume of bioaugmented culture resulted in methane production rates of 196.18 mL g−1 Volatile Solids (VS) and 245.88 mL g−1 VS after 30 and 60 days of AD, respectively. These rates were not significantly different from the control reactors (30d: 205.94 mL CH4 g−1 VS and 60d: 230.26 mL CH4 g−1 VS) operating without ammonia toxicity. Analysis of the microbial community using 16S rRNA gene sequencing revealed the dominance of acetoclastic methanogen members from the genus Methanosaeta in all reactors.

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Section: Resultsmentioning

confidence: 99%

Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition

et al. 2023

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“…Consequently, this leads to reduced methane production. Elevated nitrogen levels can promote the proliferation of ammonia-tolerant microorganisms at the expense of methanogens, disturbing the equilibrium necessary for optimal anaerobic digestion [70]. Combining substrates that have different carbonto-nitrogen ratios can assist in attaining the most favourable ratio.…”

Section: Biogas Composition Of Various Substratesmentioning

confidence: 99%

Investigating Methane, Carbon Dioxide, Ammonia, and Hydrogen Sulphide Content in Agricultural Waste during Biogas Production

Sihlangu,

Luseba,

Regnier

et al. 2024

Sustainability

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The agricultural industry produces a substantial quantity of organic waste, and finding a suitable method for disposing of this highly biodegradable solid waste is a difficult task. The utilisation of anaerobic digestion for agricultural waste is a viable technological solution for both renewable energy production (biogas) and waste treatment. The primary objective of the study was to assess the composition of biogas, namely the percentages of methane, carbon dioxide, ammonia, and hydrogen sulphide. Additionally, the study aimed to quantify the amount of biogas produced and determine the methane yield (measured in NmL/g VS) from different agricultural substrates. The biochemical methane potential (BMP) measurements were conducted in triplicate using the BPC Instruments AMPTS II instrument. The substrates utilised in the investigation were chosen based on their accessibility. The substrates used in this study comprise cattle manure, chicken manure, pig manure, tomato plants, tomatoes, cabbage, mixed fruits, mixed vegetables, dog food, and a co-digestion of mixed vegetables, fruits, and dog food (MVMFDF). Prior to the cleaning process, the makeup of the biogas was assessed using the BIOGAS 5000, a Geotech Analyser. The AMPTS II flow cell automatically monitored and recorded the volume of bio-methane produced after the cleaning stage. The data were examined using the Minitab-17 software. The co-digestion of mixed vegetables, mixed fruits, and dog food (MVMFDF) resulted in the highest methane level of 77.4%, followed by mixed fruits at 76.6%, pig manure at 72.57%, and mixed vegetables at 70.1%. The chicken manure exhibited the greatest levels of ammonia (98.0 ppm) and hydrogen sulphide (589 ppm). Chicken manure had the highest hydrogen sulphide level, followed by pig manure (540 ppm), tomato plants (485 ppm), mixed fruits (250 ppm), and MVMFDF (208 ppm). Ultimately, the makeup of biogas is greatly affected by the unique qualities of each substrate. Substrates containing elevated quantities of hydrogen sulphide, such as chicken manure, require the process of biogas scrubbing. This is because they contain substantial amounts of ammonia and hydrogen sulphide, which can cause corrosion to the equipment in biogas plants. This emphasises the crucial need to meticulously choose substrates, with a specific focus on their organic composition and their capacity to generate elevated methane levels while minimising contaminants. Substrates with a high organic content, such as agricultural waste, are optimal for maximising the production of methane. Furthermore, the implementation of biogas scrubbing procedures is essential for efficiently decreasing carbon dioxide and hydrogen sulphide levels in biogas. By considering and tackling these problems, the effectiveness of biogas generation can be enhanced and its ecological consequences alleviated. This strategy facilitates the advancement of biogas as a sustainable energy source, hence contributing to the attainment of sustainable development goals (SDGs).

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“…Biodegradation of nitrogenous materials produces ammonia as a byproduct. Above threshold concentrations, free ammonia nitrogen (FAN), dependent on pH and temperature [61], is a significant inhibitor species of total ammonia in an anaerobic digester [62]. Thus, the excess accumulation of ammonia in the digester increases pH and finally causes the failure of a process [63].…”

Section: Ammoniamentioning

confidence: 99%

Impacts of Anaerobic Co-Digestion on Different Influencing Parameters: A Critical Review

2022

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Lignocellulosic feedstocks are year-round, available bio-residues that are the right candidates for counteracting the energy crises and global warming facing the world today. However, lignin leads to a slow hydrolysis rate and is a major bottleneck for biogas production via anaerobic digestion. Anaerobic co-digestion (AcoD) is an economical method available, which overcomes the limitation of a single feedstock’s properties in an anaerobic digestion process. This paper critically reviews the impacts of co-digestion on lignocellulosic biomass degradation, process stability, various working parameters, and microbial activities that improve methane yields. A combination of compatible substrates is chosen to improve the biomethane yield and conversion rate of organic matter. AcoD is a promising method in the delignification of lignocellulosic biomass as an acid pretreatment. Ultimate practices to control the impact of co-digestion on system performances include co-feed selection, in terms of both carbon-to-nitrogen (C/N) and mixing ratios, and other operating conditions. A detailed analysis is performed using data reported in the recent past to assess the sensitivity of influencing parameters on the resultant biogas yield. For the investigators motivated by the basic principles of AcoD technology, this review paper generates baseline data for further research work around co-digestion.

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Microbial adaptation and response to high ammonia concentrations and precipitates during anaerobic digestion under psychrophilic and mesophilic conditions

Cited by 15 publications

References 63 publications

Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition

Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition

Investigating Methane, Carbon Dioxide, Ammonia, and Hydrogen Sulphide Content in Agricultural Waste during Biogas Production

Impacts of Anaerobic Co-Digestion on Different Influencing Parameters: A Critical Review

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