Microbial activity and community structure of a soil after heavy metal contamination in a model forest ecosystem

Frey, Beat; Stemmer, Michael; Widmer, Franco; Luster, J.; Sperisen, Christoph

doi:10.1016/j.soilbio.2005.11.032

Cited by 111 publications

(69 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Changes in microbial community structure, as determined by changes in PLFA profiles, have been reported in Cu-, Ni-, Cr-, and Zn-contaminated soils [11,12]. Therefore, the PLFA profiles were also used to investigate the effect of Cu-based fungicides on soil microbial community structure in this study.…”

Section: ⅳ Discussionmentioning

confidence: 96%

Microbial communities of apple orchard soils receiving long-term copper fungicides

Wang¹,

Zhou²

2013

Proceedings of the 2013 International Conference on Remote Sensing,Environment and Transportation Engineering

View full text Add to dashboard Cite

Abstract-The aim of this study was to investigate the effect of long-term application of Cu-based fungicides on soil microbial communities using profiling of phospholipid fatty acids (PLFAs). The results showed that the total PLFA, Gram-positive bacteria, Gram-negative bacteria, fungal PLFA contents, and fungi/bacteria ratio varied among the orchard soils with different planting-time.Stepwise regression analysis between PC1 and PC2 scores for PLFA data and soil properties showed that the change of PC2 was mainly due to the available soil K (r = 0.84, p < 0.05), which suggested that soil fertilization could reduce the negative effects of Cu to the soil environment.

show abstract

Section: ⅳ Discussionmentioning

confidence: 96%

Microbial communities of apple orchard soils receiving long-term copper fungicides

Wang¹,

Zhou²

2013

Proceedings of the 2013 International Conference on Remote Sensing,Environment and Transportation Engineering

View full text Add to dashboard Cite

show abstract

“…A total of 14 environmental variables were adopted as the explanatory variables in RDA: three heavy metals (copper, zinc and lead) which were all exceeded their Effects-Range-Low (ERL) values, two organic pollutants (TBT and total PAHs), five physicochemical properties (pH, moisture, TOM, TKN and TKP), three microbial properties (THB, DHA and CAT) and one descriptive variable assigned to characterize the sampling time (Time). A forward selection followed by the Monte Carlo permutation test was performed to test which variable had a significant influence on the microbial EL-FAME composition (Frey et al, 2006;Andersen et al, 2010). A simpler model was built to select a subset of main important factors with a low extent of cross correlation, which would help to avoid the "arch effect" in the sample scatter plot (Lepš and Šmilauer, 2003).…”

Section: Discussionmentioning

confidence: 99%

Natural attenuation of contaminated marine sediments from an old floating dock Part II: Changes of sediment microbial community structure and its relationship with environmental variables

Wang

Tam

2012

Science of The Total Environment

View full text Add to dashboard Cite

“…Nine month old plants were taken from the pots and shaken carefully to remove the nonadhering soil particles. Then a brush was used to gently remove the adhering rhizosphere soil particles from plant roots, which were passed through a 1 mm sieve and stored in a DNA extraction buffer (Frey et al 2006) at -20°C until analysis. Soil DNA from the rhizosphere samples was prepared by a bead beating procedure (Frey et al 2008).…”

Section: Microbial Measurementsmentioning

confidence: 99%

“…Prior to the T-RFLP analysis, digests were desalted with Montage SEQ96 sequencing reaction clean-up kit (Millipore Corporation), according to the manufacturer's instructions. T-RFLP analyses were performed according to Frey et al (2006). Two microlitres of digested PCR products were analysed along with 0.2 ll of internal size standard ROX500 (Applied Biosystems, Foster City, CA, USA) and 12 ll HiDi formamide (Applied Biosystems) on an ABI Prism 310 Genetic Analyzer (Applied Biosystems) with 36 cm capillaries filled with POP-4 polymer.…”

Section: Terminal Restriction Enzyme Fragment Length Polymorphism Anamentioning

confidence: 99%

Species-specific effects of polyploidisation and plant traits of Centaurea maculosa and Senecio inaequidens on rhizosphere microorganisms

et al. 2010

Self Cite

View full text Add to dashboard Cite

Invasive plant species represent a threat to terrestrial ecosystems, but their effects on the soil biota and the mechanisms involved are not yet well understood. Many invasive species have undergone polyploidisation, leading to the coexistence of various cytotypes in the native range, whereas, in most cases, only one cytotype is present in the introduced range. Since genetic variation within a species can modify soil rhizosphere communities, we studied the effects of different cytotypes and ranges (native diploid, native tetraploid and introduced tetraploid) of Centaurea maculosa and Senecio inaequidens on microbial biomass carbon, rhizosphere total DNA content and bacterial communities of a standard soil in relation to plant functional traits. There was no overall significant difference in microbial biomass between cytotypes. The variation of rhizosphere total DNA content and bacterial community structure according to cytotype was species specific. The rhizosphere DNA content of S. inaequidens decreased with polyploidisation in the native range but did not vary for C. maculosa. In contrast, the bacterial community structure of C. maculosa was affected by polyploidisation and its diversity increased, whereas there was no significant change for S. inaequidens. Traits of S. inaequidens were correlated to the rhizosphere biota. Bacterial diversity and total DNA content were positively correlated with resource allocation to belowground growth and late flowering, whereas microbial biomass carbon was negatively correlated to investment in reproduction. There were no correlations between traits of the cytotypes of C. maculosa and corresponding rhizosphere soil biota. This study shows that polyploidisation may affect rhizosphere bacterial community composition, but that effects vary among plant species. Such changes may contribute to the success of invasive polyploid genotypes in the introduced range.

show abstract

Microbial activity and community structure of a soil after heavy metal contamination in a model forest ecosystem

Cited by 111 publications

References 59 publications

Microbial communities of apple orchard soils receiving long-term copper fungicides

Microbial communities of apple orchard soils receiving long-term copper fungicides

Natural attenuation of contaminated marine sediments from an old floating dock Part II: Changes of sediment microbial community structure and its relationship with environmental variables

Species-specific effects of polyploidisation and plant traits of Centaurea maculosa and Senecio inaequidens on rhizosphere microorganisms

Contact Info

Product

Resources

About