Microbial acetylation of M factor of virginiamycin.

Meester, Conrad De; Rondelet, J

doi:10.7164/antibiotics.29.1297

Cited by 25 publications

(12 citation statements)

References 4 publications

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“…An acetyltransferase that inactivates the A compounds was detected in the lysates of two pristinamycin-resistant staphylococcal isolates (10,22). The gene encoding this enzyme, vat, was recently isolated from a staphylococcal plasmid and sequenced (3).…”

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confidence: 99%

Analysis of pristinamycin-resistant Staphylococcus epidermidis isolates responsible for an outbreak in a Parisian hospital

Loncle

Casetta

Buu-Hoï

et al. 1993

Antimicrob Agents Chemother

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In 1990, over a 6-month period, an increase from 1 to 10%Y in the incidence of pristinamycin resistance among coagulase-negative staphylococci was observed in four intensive care units of a Parisian hospital.Twenty-three such isolates, as well as 25 pristinamycin-susceptible Staphylococcus epidermidis isolates, were collected and typed by analyzing various bacterial constituents. Two structurally related plasmids of 7.3 and 14.3 kb, carrying the gene vga encoding resistance to pristinamycin, were detected in the 23 pristinamycinresistant coagulase-negative staphylococci which were identified as S. epidermidis. Although related by numerous common characteristics, 20 of these 23 isolates could be divided into two types, A (17 isolates) and B (three isolates). These types were characterized on the basis of their plasmid contents and hybridization patterns obtained when the EcoRI-digested DNA was probed with plasmid pIP1551 containing an internal fragment of the insertion sequence IS256. These findings suggest that the dissemination of type A epidemic strains was, in large part, responsible for the outbreaL

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mentioning

confidence: 99%

Analysis of pristinamycin-resistant Staphylococcus epidermidis isolates responsible for an outbreak in a Parisian hospital

Loncle

Casetta

Buu-Hoï

et al. 1993

Antimicrob Agents Chemother

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“…The quinupristin-dalfopristin combination has proved useful in fighting infections produced by dangerous antibiotic-resistant strains of staphylococci or enterococci (14,16,22). Resistance to the class A streptogramins takes place either by active efflux mediated by the gene vga in staphylococci (3) or by inactivation via enzymatic acetylation of the antibiotic (12). This inactivation is catalyzed by streptogramin (virginiamycin) acetyltransferases, the products of sat or vat genes (1,5,23).…”

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Identification of a Streptogramin A Acetyltransferase Gene in the Chromosome of Yersinia enterocolitica

Seoane

Lobo

2000

Antimicrob Agents Chemother

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“…It functions by binding to the 50 s subunit of the ribosome and inhibiting protein synthesis. Resistance to virginiamycin is associated with enzymes encoded by the genes vat(D) and vat(E) that inactivate the A component of virginiamycin (factor M) by acetylation of the hydroxyl group [10,24,29]. We screened all Lactobacillus isolates for the presence of vat(D) and vat(E).…”

Section: Screening For Antimicrobial Resistance Genesmentioning

confidence: 99%

Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants

Bischoff

Skinner-Nemec

Leathers

2007

J Ind Microbiol Biotechnol

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Bacterial contamination of commercial fermentation cultures is a common and costly problem to the fuel ethanol industry. Antimicrobials such as virginiamycin (VIR) and penicillin (PEN) are frequently used to control contamination but there are little data available on the susceptibility of bacterial contaminants to these agents. A survey of bacterial contaminants from a wet-mill ethanol plant with no history of using antibiotics and a dry-grind facility that periodically doses with VIR found that the majority of contaminants were species of Lactobacillus. Thirty-seven isolates of Lactobacillus species from the wet-mill and 42 isolates from the dry-grind facility were tested for antimicrobial susceptibility using broth dilution and agar dilution methods. In general, the Lactobacillus isolates from the dry-grind plant had higher minimum inhibitory concentrations (MICs) for the tested agents than the isolates from the wet-mill facility. The MIC(90) for VIR was 4 microg/ml for the dry-grind isolates versus 0.25 microg/ml for the wet-mill isolates; and for PEN, the MIC(90)'s were >8 and 2 microg/ml for the dry-grind and wet-mill isolates, respectively. Sixteen Lactobacillus isolates from the dry-grind plant but none from the wet-mill possessed vatE, a gene that encodes a streptogramin acetyltransferase associated with resistance to virginiamycin. Despite decreased susceptibility to virginiamycin, most dry-grind isolates had MICs lower than the maximal recommended application rate of 6 ppm.

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Microbial acetylation of M factor of virginiamycin.

Cited by 25 publications

References 4 publications

Analysis of pristinamycin-resistant Staphylococcus epidermidis isolates responsible for an outbreak in a Parisian hospital

Analysis of pristinamycin-resistant Staphylococcus epidermidis isolates responsible for an outbreak in a Parisian hospital

Identification of a Streptogramin A Acetyltransferase Gene in the Chromosome of Yersinia enterocolitica

Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants

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